5lbx - Revision history

OCA at 08:55, 11 October 2023

OCA — Wed, 11 Oct 2023 08:55:46 GMT

←Older revision		Revision as of 08:55, 11 October 2023
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	<StructureSection load='5lbx' size='340' side='right'caption='[[5lbx]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='5lbx' size='340' side='right'caption='[[5lbx]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lbx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LBX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LBX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lbx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_tropicalis Candida tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LBX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COO:CROTONYL+COENZYME+A'>COO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5lbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lbx OCA], [~~http~~://pdbe.org/5lbx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lbx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lbx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lbx ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COO:CROTONYL+COENZYME+A'>COO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lbx OCA], [https://pdbe.org/5lbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lbx RCSB], [https://www.ebi.ac.uk/pdbsum/5lbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lbx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ETR1_CANTR ETR1_CANTR]] Required for respiration and the maintenance of the mitochondrial compartment. May have a role in the mitochondrial synthesis of fatty acids.<ref>PMID:11509667</ref>	+	[https://www.uniprot.org/uniprot/ETR1_CANTR ETR1_CANTR] Required for respiration and the maintenance of the mitochondrial compartment. May have a role in the mitochondrial synthesis of fatty acids.<ref>PMID:11509667</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Candida tropicalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Erb~~, T J~~]]	+	[[Category: Erb TJ]]
-	[[Category: Rosenthal~~, R G~~]]	+	[[Category: Rosenthal RG]]
-	[[Category: Shima, S]]	+	[[Category: Shima S]]
-	[[Category: Voegeli, B]]	+	[[Category: Voegeli B]]
-	[[Category: Wagner, T]]	+	[[Category: Wagner T]]
-	~~[[Category: Crotonyl-coa]]~~	+
-	~~[[Category: Enzyme]]~~	+
-	~~[[Category: Mitochondria]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Nadp]]~~	+
-	~~[[Category: Negative catalysis]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Reductase~~]]	+

OCA at 07:06, 5 August 2020

OCA — Wed, 05 Aug 2020 07:06:34 GMT

←Older revision		Revision as of 07:06, 5 August 2020
Line 1:		Line 1:

	==Structure of the T175V Etr1p mutant in the trigonal form P312 in complex with NADP and crotonyl-CoA==		==Structure of the T175V Etr1p mutant in the trigonal form P312 in complex with NADP and crotonyl-CoA==
-	<StructureSection load='5lbx' size='340' side='right' caption='[[5lbx]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='5lbx' size='340' side='right'caption='[[5lbx]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lbx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LBX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LBX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lbx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LBX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LBX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COO:CROTONYL+COENZYME+A'>COO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COO:CROTONYL+COENZYME+A'>COO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lbx OCA], [http://pdbe.org/5lbx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lbx RCSB], [http://www.ebi.ac.uk/pdbsum/5lbx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lbx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lbx OCA], [http://pdbe.org/5lbx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lbx RCSB], [http://www.ebi.ac.uk/pdbsum/5lbx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lbx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5lbx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lbx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Enoyl-Acyl-Carrier Protein Reductase 3D structures\|Enoyl-Acyl-Carrier Protein Reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Erb, T J]]		[[Category: Erb, T J]]
	[[Category: Rosenthal, R G]]		[[Category: Rosenthal, R G]]

OCA at 07:06, 24 May 2017

OCA — Wed, 24 May 2017 07:06:23 GMT

←Older revision		Revision as of 07:06, 24 May 2017
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ETR1_CANTR ETR1_CANTR]] Required for respiration and the maintenance of the mitochondrial compartment. May have a role in the mitochondrial synthesis of fatty acids.<ref>PMID:11509667</ref>		[[http://www.uniprot.org/uniprot/ETR1_CANTR ETR1_CANTR]] Required for respiration and the maintenance of the mitochondrial compartment. May have a role in the mitochondrial synthesis of fatty acids.<ref>PMID:11509667</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzymes are highly specific biocatalysts, yet they can promote unwanted side reactions. Here we investigated the factors that direct catalysis in the enoyl-thioester reductase Etr1p. We show that a single conserved threonine is essential to suppress the formation of a side product that would otherwise act as a high-affinity inhibitor of the enzyme. Substitution of this threonine with isosteric valine increases side-product formation by more than six orders of magnitude, while decreasing turnover frequency by only one order of magnitude. Our results show that the promotion of wanted reactions and the suppression of unwanted side reactions operate independently at the active site of Etr1p, and that the active suppression of side reactions is highly conserved in the family of medium-chain dehydrogenases/reductases (MDRs). Our discovery emphasizes the fact that the active destabilization of competing transition states is an important factor during catalysis that has implications for the understanding and the de novo design of enzymes.
		+
		+	A conserved threonine prevents self-intoxication of enoyl-thioester reductases.,Rosenthal RG, Vogeli B, Wagner T, Shima S, Erb TJ Nat Chem Biol. 2017 May 15. doi: 10.1038/nchembio.2375. PMID:28504678<ref>PMID:28504678</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5lbx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:58, 10 May 2017

OCA — Wed, 10 May 2017 12:58:06 GMT

←Older revision		Revision as of 12:58, 10 May 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5lbx ~~is ON HOLD until Paper Publication~~	+	==Structure of the T175V Etr1p mutant in the trigonal form P312 in complex with NADP and crotonyl-CoA==
-		+	<StructureSection load='5lbx' size='340' side='right' caption='[[5lbx]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~Authors~~: ~~Wagner, T~~., ~~Rosenthal~~, R.G., ~~Voegeli~~, B., ~~Shima~~, S., ~~Erb, T~~.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5lbx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LBX FirstGlance]. <br>
-	~~Description~~: ~~Structure~~ of the ~~T175V Etr1p mutant~~ in the ~~trigonal form P312 in complex with NADP and crotonyl-CoA~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COO:CROTONYL+COENZYME+A'>COO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lbx OCA], [http://pdbe.org/5lbx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lbx RCSB], [http://www.ebi.ac.uk/pdbsum/5lbx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lbx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ETR1_CANTR ETR1_CANTR]] Required for respiration and the maintenance of the mitochondrial compartment. May have a role in the mitochondrial synthesis of fatty acids.<ref>PMID:11509667</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Erb, T J]]
		+	[[Category: Rosenthal, R G]]
	[[Category: Shima, S]]		[[Category: Shima, S]]
		+	[[Category: Voegeli, B]]
	[[Category: Wagner, T]]		[[Category: Wagner, T]]
-	[[Category: ~~Erb, T.J~~]]	+	[[Category: Crotonyl-coa]]
-	[[Category: ~~Rosenthal, R.G~~]]	+	[[Category: Enzyme]]
-	[[Category: ~~Voegeli, B~~]]	+	[[Category: Mitochondria]]
		+	[[Category: Mutant]]
		+	[[Category: Nadp]]
		+	[[Category: Negative catalysis]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Reductase]]

OCA: Protected "5lbx" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2016 00:56:06 GMT

Protected "5lbx" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:56, 13 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lbx is ON HOLD until Paper Publication Authors: Wagner, T., Rosenthal, R.G., Voegeli, B., Shima, S., Erb, T.J. Description: Structure of the T175V...

OCA — Wed, 13 Jul 2016 00:56:04 GMT

New page: '''Unreleased structure''' The entry 5lbx is ON HOLD until Paper Publication Authors: Wagner, T., Rosenthal, R.G., Voegeli, B., Shima, S., Erb, T.J. Description: Structure of the T175V...

New page

'''Unreleased structure'''

The entry 5lbx is ON HOLD until Paper Publication

Authors: Wagner, T., Rosenthal, R.G., Voegeli, B., Shima, S., Erb, T.J.

Description: Structure of the T175V Etr1p mutant in the trigonal form P312 in complex with NADP and crotonyl-CoA
[[Category: Unreleased Structures]]
[[Category: Shima, S]]
[[Category: Wagner, T]]
[[Category: Erb, T.J]]
[[Category: Rosenthal, R.G]]
[[Category: Voegeli, B]]