5lc6 - Revision history

OCA at 08:55, 11 October 2023

OCA — Wed, 11 Oct 2023 08:55:56 GMT

←Older revision		Revision as of 08:55, 11 October 2023
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	<StructureSection load='5lc6' size='340' side='right'caption='[[5lc6]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5lc6' size='340' side='right'caption='[[5lc6]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lc6]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Diocu Diocu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LC6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LC6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lc6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LC6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lc6 OCA], [~~http~~://pdbe.org/5lc6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lc6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lc6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lc6 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lc6 OCA], [https://pdbe.org/5lc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lc6 RCSB], [https://www.ebi.ac.uk/pdbsum/5lc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lc6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU]] Taste-modifying protein; intensely sweet-tasting protein.	+	[https://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.[https://www.uniprot.org/uniprot/MONA_DIOCU MONA_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Diocu~~]]	+	[[Category: Dioscoreophyllum cumminsii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Merlino, A]]	+	[[Category: Merlino A]]
-	[[Category: Pica, A]]	+	[[Category: Pica A]]
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: Sweet protein~~]]	+

OCA at 06:27, 16 October 2019

OCA — Wed, 16 Oct 2019 06:27:40 GMT

←Older revision		Revision as of 06:27, 16 October 2019
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	==Crystal structure of a single chain monellin mutant: Q28K/C41S/Y65R-MNEI==		==Crystal structure of a single chain monellin mutant: Q28K/C41S/Y65R-MNEI==
-	<StructureSection load='5lc6' size='340' side='right' caption='[[5lc6]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5lc6' size='340' side='right'caption='[[5lc6]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lc6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LC6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LC6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lc6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LC6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LC6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lc6 OCA], [http://pdbe.org/5lc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lc6 RCSB], [http://www.ebi.ac.uk/pdbsum/5lc6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lc6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lc6 OCA], [http://pdbe.org/5lc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lc6 RCSB], [http://www.ebi.ac.uk/pdbsum/5lc6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lc6 ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Diocu]]
		+	[[Category: Large Structures]]
	[[Category: Merlino, A]]		[[Category: Merlino, A]]
	[[Category: Pica, A]]		[[Category: Pica, A]]
	[[Category: Plant protein]]		[[Category: Plant protein]]
	[[Category: Sweet protein]]		[[Category: Sweet protein]]

OCA at 21:36, 5 October 2016

OCA — Wed, 05 Oct 2016 21:36:36 GMT

←Older revision		Revision as of 21:36, 5 October 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5lc6 is ~~ON HOLD~~	+	==Crystal structure of a single chain monellin mutant: Q28K/C41S/Y65R-MNEI==
		+	<StructureSection load='5lc6' size='340' side='right' caption='[[5lc6]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lc6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LC6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LC6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lc6 OCA], [http://pdbe.org/5lc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lc6 RCSB], [http://www.ebi.ac.uk/pdbsum/5lc6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lc6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU]] Taste-modifying protein; intensely sweet-tasting protein.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sweet proteins are a family of proteins with no structure or sequence homology, able to elicit a sweet sensation in humans through their interaction with the dimeric T1R2-T1R3 sweet receptor. In particular, monellin and its single chain derivative (MNEI) are among the sweetest proteins known to men. Starting from a careful analysis of the surface electrostatic potentials, we have designed new mutants of MNEI with enhanced sweetness. Then, we have included in the most promising variant the stabilising mutation E23Q, obtaining a construct with enhanced performances, which combines extreme sweetness to high, pH-independent, thermal stability. The resulting mutant, with a sweetness threshold of only 0.28 mg/L (25 nM) is the strongest sweetener known to date. All the new proteins have been produced and purified and the structures of the most powerful mutants have been solved by X-ray crystallography. Docking studies have then confirmed the rationale of their interaction with the human sweet receptor, hinting at a previously unpredicted role of plasticity in said interaction.

-	~~Authors~~: ~~Pica~~, A., Merlino, A.	+	Sweeter and stronger: enhancing sweetness and stability of the single chain monellin MNEI through molecular design.,Leone S, Pica A, Merlino A, Sannino F, Temussi PA, Picone D Sci Rep. 2016 Sep 23;6:34045. doi: 10.1038/srep34045. PMID:27658853<ref>PMID:27658853</ref>

-	~~Description: Crystal structure of~~ a ~~single chain monellin mutant~~: ~~Q28K~~/~~C41S~~/~~Y65R-MNEI~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5lc6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Merlino, A]]		[[Category: Merlino, A]]
	[[Category: Pica, A]]		[[Category: Pica, A]]
		+	[[Category: Plant protein]]
		+	[[Category: Sweet protein]]

OCA: Protected "5lc6" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2016 00:56:47 GMT

Protected "5lc6" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:56, 13 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lc6 is ON HOLD Authors: Pica, A., Merlino, A. Description: Crystal structure of a single chain monellin mutant: Q28K/C41S/Y65R-MNEI [[Category: Un...

OCA — Wed, 13 Jul 2016 00:56:46 GMT

New page: '''Unreleased structure''' The entry 5lc6 is ON HOLD Authors: Pica, A., Merlino, A. Description: Crystal structure of a single chain monellin mutant: Q28K/C41S/Y65R-MNEI [[Category: Un...

New page

'''Unreleased structure'''

The entry 5lc6 is ON HOLD

Authors: Pica, A., Merlino, A.

Description: Crystal structure of a single chain monellin mutant: Q28K/C41S/Y65R-MNEI
[[Category: Unreleased Structures]]
[[Category: Merlino, A]]
[[Category: Pica, A]]