5lcn - Revision history

OCA at 08:56, 11 October 2023

OCA — Wed, 11 Oct 2023 08:56:18 GMT

←Older revision		Revision as of 08:56, 11 October 2023
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	<StructureSection load='5lcn' size='340' side='right'caption='[[5lcn]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='5lcn' size='340' side='right'caption='[[5lcn]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lcn]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pyrfu Pyrfu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LCN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LCN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lcn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LCN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[4g59\|4g59]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">PF2001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lcn OCA], [https://pdbe.org/5lcn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lcn RCSB], [https://www.ebi.ac.uk/pdbsum/5lcn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lcn ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lcn OCA], [~~http~~://pdbe.org/5lcn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lcn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lcn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lcn ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8TZJ1_PYRFU Q8TZJ1_PYRFU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pyrfu~~]]	+	[[Category: Pyrococcus furiosus DSM 3638]]
-	[[Category: Reverter, D]]	+	[[Category: Reverter D]]
-	[[Category: Varejao, N]]	+	[[Category: Varejao N]]
-	~~[[Category: Esterase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Themophilic~~]]	+

OCA at 06:01, 12 June 2019

OCA — Wed, 12 Jun 2019 06:01:19 GMT

←Older revision		Revision as of 06:01, 12 June 2019
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	==STRUCTURE OF THE PYROCOCCUS FURIOSUS ESTERASE PF2001 WITH SPACE GROUP P212121==		==STRUCTURE OF THE PYROCOCCUS FURIOSUS ESTERASE PF2001 WITH SPACE GROUP P212121==
-	<StructureSection load='5lcn' size='340' side='right' caption='[[5lcn]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='5lcn' size='340' side='right'caption='[[5lcn]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5lcn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LCN FirstGlance]. <br>		<table><tr><td colspan='2'>[[5lcn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LCN FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pyrfu]]		[[Category: Pyrfu]]
-	[[Category: ~~REVERTER~~, D]]	+	[[Category: Reverter, D]]
-	[[Category: ~~VAREJAO~~, N]]	+	[[Category: Varejao, N]]
	[[Category: Esterase]]		[[Category: Esterase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Themophilic]]		[[Category: Themophilic]]

OCA at 08:08, 17 January 2018

OCA — Wed, 17 Jan 2018 08:08:53 GMT

←Older revision		Revision as of 08:08, 17 January 2018
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	<StructureSection load='5lcn' size='340' side='right' caption='[[5lcn]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='5lcn' size='340' side='right' caption='[[5lcn]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lcn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LCN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lcn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LCN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g59\|4g59]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g59\|4g59]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PF2001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lcn OCA], [http://pdbe.org/5lcn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lcn RCSB], [http://www.ebi.ac.uk/pdbsum/5lcn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lcn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lcn OCA], [http://pdbe.org/5lcn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lcn RCSB], [http://www.ebi.ac.uk/pdbsum/5lcn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lcn ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lipases and esterases constitute a group of enzymes that catalyze the hydrolysis or synthesis of ester bonds. A major biotechnological interest corresponds to thermophilic esterases, due to their intrinsic stability at high temperatures. The Pf2001 esterase from Pyrococcus furiosus reaches its optimal activity between 70 degrees C and 80 degrees C. The crystal structure of the Pf2001 esterase shows two different conformations: monomer and dimer. The structures reveal important rearrangements in the "cap" subdomain between monomer and dimer, by the formation of an extensive intertwined helical interface. Moreover, the dimer interface is essential for the formation of the hydrophobic channel for substrate selectivity, as confirmed by mutagenesis and kinetic analysis. We also provide evidence for dimer formation at high temperatures, a process that correlates with its enzymatic activation. Thus, we propose a temperature-dependent activation mechanism of the Pf2001 esterase via dimerization that is necessary for the substrate channel formation in the active-site cleft.
		+
		+	Structural Mechanism for the Temperature-Dependent Activation of the Hyperthermophilic Pf2001 Esterase.,Varejao N, De-Andrade RA, Almeida RV, Anobom CD, Foguel D, Reverter D Structure. 2017 Dec 28. pii: S0969-2126(17)30403-3. doi:, 10.1016/j.str.2017.12.004. PMID:29307486<ref>PMID:29307486</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5lcn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pyrfu]]
	[[Category: REVERTER, D]]		[[Category: REVERTER, D]]
	[[Category: VAREJAO, N]]		[[Category: VAREJAO, N]]

OCA at 07:36, 6 October 2017

OCA — Fri, 06 Oct 2017 07:36:11 GMT

←Older revision		Revision as of 07:36, 6 October 2017
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-	'''Unreleased structure'''

-	~~The entry 5lcn is ON HOLD until Jun 22 2018~~	+	==STRUCTURE OF THE PYROCOCCUS FURIOSUS ESTERASE PF2001 WITH SPACE GROUP P212121==
-		+	<StructureSection load='5lcn' size='340' side='right' caption='[[5lcn]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~Authors: VAREJAO, N., REVERTER, D.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5lcn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LCN FirstGlance]. <br>
-	~~Description:~~ STRUCTURE OF THE PYROCOCCUS FURIOSUS ESTERASE PF2001 WITH SPACE GROUP P212121	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g59\|4g59]]</td></tr>
-	[[Category: ~~Reverter~~, D]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lcn OCA], [http://pdbe.org/5lcn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lcn RCSB], [http://www.ebi.ac.uk/pdbsum/5lcn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lcn ProSAT]</span></td></tr>
-	[[Category: ~~Varejao~~, N]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: REVERTER, D]]
		+	[[Category: VAREJAO, N]]
		+	[[Category: Esterase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Themophilic]]

OCA: Protected "5lcn" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2016 00:58:52 GMT

Protected "5lcn" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:58, 13 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lcn is ON HOLD until Jun 22 2018 Authors: VAREJAO, N., REVERTER, D. Description: STRUCTURE OF THE PYROCOCCUS FURIOSUS ESTERASE PF2001 WITH SPACE G...

OCA — Wed, 13 Jul 2016 00:58:50 GMT

New page: '''Unreleased structure''' The entry 5lcn is ON HOLD until Jun 22 2018 Authors: VAREJAO, N., REVERTER, D. Description: STRUCTURE OF THE PYROCOCCUS FURIOSUS ESTERASE PF2001 WITH SPACE G...

New page

'''Unreleased structure'''

The entry 5lcn is ON HOLD until Jun 22 2018

Authors: VAREJAO, N., REVERTER, D.

Description: STRUCTURE OF THE PYROCOCCUS FURIOSUS ESTERASE PF2001 WITH SPACE GROUP P212121
[[Category: Unreleased Structures]]
[[Category: Reverter, D]]
[[Category: Varejao, N]]