5le4 - Revision history

OCA at 08:57, 11 October 2023

OCA — Wed, 11 Oct 2023 08:57:25 GMT

←Older revision		Revision as of 08:57, 11 October 2023
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	<StructureSection load='5le4' size='340' side='right'caption='[[5le4]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='5le4' size='340' side='right'caption='[[5le4]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5le4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LE4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LE4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5le4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LE4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5le4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5le4 OCA], [~~http~~://pdbe.org/5le4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5le4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5le4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5le4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5le4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5le4 OCA], [https://pdbe.org/5le4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5le4 RCSB], [https://www.ebi.ac.uk/pdbsum/5le4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5le4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Batyuk, A]]	+	[[Category: Batyuk A]]
-	[[Category: Mittl~~, P R~~]]	+	[[Category: Mittl PR]]
-	[[Category: Plueckthun, A]]	+	[[Category: Plueckthun A]]
-	[[Category: Wu, Y]]	+	[[Category: Wu Y]]
-	~~[[Category: De novo protein]]~~	+
-	~~[[Category: Designed ankyrin repeat protein]]~~	+
-	~~[[Category: Protein design]]~~	+
-	~~[[Category: Protein engineering]]~~	+
-	~~[[Category: Rigid domain fusion]]~~	+
-	~~[[Category: X-ray crystallography~~]]	+

OCA at 08:11, 20 March 2019

OCA — Wed, 20 Mar 2019 08:11:58 GMT

←Older revision		Revision as of 08:11, 20 March 2019
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	==Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_11_D12==		==Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_11_D12==
-	<StructureSection load='5le4' size='340' side='right' caption='[[5le4]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='5le4' size='340' side='right'caption='[[5le4]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5le4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LE4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5le4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LE4 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5le4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5le4 OCA], [http://pdbe.org/5le4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5le4 RCSB], [http://www.ebi.ac.uk/pdbsum/5le4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5le4 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5le4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5le4 OCA], [http://pdbe.org/5le4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5le4 RCSB], [http://www.ebi.ac.uk/pdbsum/5le4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5le4 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multivalent binding proteins can gain biological activities beyond what is inherent in the individual binders, by bringing together different target molecules, restricting their conformational flexibility or changing their subcellular localization. In this study, we demonstrate a method to build up rigid multivalent and multispecific scaffolds by exploiting the modular nature of a repeat protein scaffold and avoiding flexible linkers. We use DARPins (Designed Ankyrin Repeat Proteins), synthetic binding proteins based on the Ankyrin-repeat protein scaffold, as binding units. Their ease of in vitro selection, high production yield and stability make them ideal specificity-conferring building blocks for the design of more complex constructs. C- and N-terminal DARPin capping repeats were re-designed to be joined by a shared helix in such a way that rigid connector modules are formed. This allows us to join two or more DARPins in predefined geometries without compromising their binding affinities and specificities. Nine connector modules with distinct geometries were designed; for eight of these we were able to confirm the structure by X-ray crystallography, while only one did not crystallize. The bispecific constructs were all able to bind both target proteins simultaneously.
		+
		+	Rigidly connected multispecific artificial binders with adjustable geometries.,Wu Y, Batyuk A, Honegger A, Brandl F, Mittl PRE, Pluckthun A Sci Rep. 2017 Sep 11;7(1):11217. doi: 10.1038/s41598-017-11472-x. PMID:28894181<ref>PMID:28894181</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5le4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Synthetic construct sequences]]		[[Category: Synthetic construct sequences]]
	[[Category: Batyuk, A]]		[[Category: Batyuk, A]]

OCA at 07:59, 13 November 2017

OCA — Mon, 13 Nov 2017 07:59:13 GMT

←Older revision		Revision as of 07:59, 13 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5le4 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_11_D12==
-		+	<StructureSection load='5le4' size='340' side='right' caption='[[5le4]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5le4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LE4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LE4 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5le4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5le4 OCA], [http://pdbe.org/5le4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5le4 RCSB], [http://www.ebi.ac.uk/pdbsum/5le4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5le4 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Synthetic construct sequences]]
		+	[[Category: Batyuk, A]]
		+	[[Category: Mittl, P R]]
		+	[[Category: Plueckthun, A]]
		+	[[Category: Wu, Y]]
		+	[[Category: De novo protein]]
		+	[[Category: Designed ankyrin repeat protein]]
		+	[[Category: Protein design]]
		+	[[Category: Protein engineering]]
		+	[[Category: Rigid domain fusion]]
		+	[[Category: X-ray crystallography]]

OCA: Protected "5le4" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2016 01:02:56 GMT

Protected "5le4" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:02, 13 July 2016

OCA: New page: '''Unreleased structure''' The entry 5le4 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 Jul 2016 01:02:55 GMT

New page: '''Unreleased structure''' The entry 5le4 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5le4 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]