5lgc - Revision history

OCA at 08:59, 11 October 2023

OCA — Wed, 11 Oct 2023 08:59:12 GMT

←Older revision		Revision as of 08:59, 11 October 2023
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	<StructureSection load='5lgc' size='340' side='right'caption='[[5lgc]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='5lgc' size='340' side='right'caption='[[5lgc]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lgc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Arthrobacter_sp.~~_aw19m34~~-1 Arthrobacter sp. ~~aw19m34~~-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lgc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arthrobacter_sp._AW19M34-1 Arthrobacter sp. AW19M34-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LGC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=CBS:DI(N~~-~~ACETYL-D-GLUCOSAMINE)'>CBS</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.09Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5lfz\|5lfz]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgc OCA], [~~http~~://pdbe.org/5lgc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lgc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lgc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgc OCA], [https://pdbe.org/5lgc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lgc RCSB], [https://www.ebi.ac.uk/pdbsum/5lgc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Arthrobacter sp. ~~aw19m34~~-1]]	+	[[Category: Arthrobacter sp. AW19M34-1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Rothweiler, U]]	+	[[Category: Rothweiler U]]
-	~~[[Category: Deacetylase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:28, 16 October 2019

OCA — Wed, 16 Oct 2019 06:28:38 GMT

←Older revision		Revision as of 06:28, 16 October 2019
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	==T48 deacetylase with substrate==		==T48 deacetylase with substrate==
-	<StructureSection load='5lgc' size='340' side='right' caption='[[5lgc]], [[Resolution\|resolution]] 2.09Å' scene=''>	+	<StructureSection load='5lgc' size='340' side='right'caption='[[5lgc]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5lgc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_sp._aw19m34-1 Arthrobacter sp. aw19m34-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGC FirstGlance]. <br>		<table><tr><td colspan='2'>[[5lgc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_sp._aw19m34-1 Arthrobacter sp. aw19m34-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGC FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Arthrobacter sp. aw19m34-1]]		[[Category: Arthrobacter sp. aw19m34-1]]
		+	[[Category: Large Structures]]
	[[Category: Rothweiler, U]]		[[Category: Rothweiler, U]]
	[[Category: Deacetylase]]		[[Category: Deacetylase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 10:06, 22 November 2017

OCA — Wed, 22 Nov 2017 10:06:12 GMT

←Older revision		Revision as of 10:06, 22 November 2017
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	<StructureSection load='5lgc' size='340' side='right' caption='[[5lgc]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='5lgc' size='340' side='right' caption='[[5lgc]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lgc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lgc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_sp._aw19m34-1 Arthrobacter sp. aw19m34-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBS:DI(N-ACETYL-D-GLUCOSAMINE)'>CBS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBS:DI(N-ACETYL-D-GLUCOSAMINE)'>CBS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5lfz\|5lfz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5lfz\|5lfz]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgc OCA], [http://pdbe.org/5lgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgc RCSB], [http://www.ebi.ac.uk/pdbsum/5lgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgc OCA], [http://pdbe.org/5lgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgc RCSB], [http://www.ebi.ac.uk/pdbsum/5lgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgc ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chitin, a polymer of beta(1-4)-linked N-acetylglucosamine found in e.g. arthropods, is a valuable resource that may be used to produce chitosan and chitooligosaccharides, two compounds with considerable industrial and biomedical potential. Deacetylating enzymes may be used to tailor the properties of chitin and its derived products. Here, we describe a novel CE4 enzyme originating from a marine Arthrobacter species (ArCE4A). Crystal structures of this novel deacetylase were determined, with and without bound chitobiose [(GlcNAc)2], and refined to 2.1 A and 1.6 A, respectively. In-depth biochemical characterization showed that ArCE4A has broad substrate specificity, with higher activity against longer oligosaccharides. Mass spectrometry-based sequencing of reaction products generated from a fully acetylated pentamer showed that internal sugars are more prone to deacetylation than the ends. These enzyme properties are discussed in the light of the structure of the enzyme-ligand complex, which adds valuable information to our still rather limited knowledge on enzyme-substrate interactions in the CE4 family.
		+
		+	Structure and function of a CE4 deacetylase isolated from a marine environment.,Tuveng TR, Rothweiler U, Udatha G, Vaaje-Kolstad G, Smalas A, Eijsink VGH PLoS One. 2017 Nov 6;12(11):e0187544. doi: 10.1371/journal.pone.0187544., eCollection 2017. PMID:29107991<ref>PMID:29107991</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5lgc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arthrobacter sp. aw19m34-1]]
	[[Category: Rothweiler, U]]		[[Category: Rothweiler, U]]
	[[Category: Deacetylase]]		[[Category: Deacetylase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 09:19, 1 November 2017

OCA — Wed, 01 Nov 2017 09:19:56 GMT

←Older revision		Revision as of 09:19, 1 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5lgc ~~is ON HOLD until Paper Publication~~	+	==T48 deacetylase with substrate==
-		+	<StructureSection load='5lgc' size='340' side='right' caption='[[5lgc]], [[Resolution\|resolution]] 2.09Å' scene=''>
-	~~Authors~~: ~~Rothweiler, U~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5lgc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGC FirstGlance]. <br>
-	~~Description~~: ~~T48 deacetylase with substrate~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBS:DI(N-ACETYL-D-GLUCOSAMINE)'>CBS</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5lfz\|5lfz]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lgc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgc OCA], [http://pdbe.org/5lgc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgc RCSB], [http://www.ebi.ac.uk/pdbsum/5lgc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgc ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Rothweiler, U]]		[[Category: Rothweiler, U]]
		+	[[Category: Deacetylase]]
		+	[[Category: Hydrolase]]

OCA: Protected "5lgc" [edit=sysop:move=sysop]

OCA — Thu, 14 Jul 2016 10:06:25 GMT

Protected "5lgc" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:06, 14 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lgc is ON HOLD until Paper Publication Authors: Rothweiler, U. Description: T48 deacetylase with substrate Category: Unreleased Structures [[C...

OCA — Thu, 14 Jul 2016 10:06:23 GMT

New page: '''Unreleased structure''' The entry 5lgc is ON HOLD until Paper Publication Authors: Rothweiler, U. Description: T48 deacetylase with substrate Category: Unreleased Structures [[C...

New page

'''Unreleased structure'''

The entry 5lgc is ON HOLD until Paper Publication

Authors: Rothweiler, U.

Description: T48 deacetylase with substrate
[[Category: Unreleased Structures]]
[[Category: Rothweiler, U]]