5lgf - Revision history

OCA at 08:59, 11 October 2023

OCA — Wed, 11 Oct 2023 08:59:16 GMT

←Older revision		Revision as of 08:59, 11 October 2023
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	==Solution structure of the N-terminal domain of Ogataea polymorpha telomerase reverse transcriptase==		==Solution structure of the N-terminal domain of Ogataea polymorpha telomerase reverse transcriptase==
-	<StructureSection load='5lgf' size='340' side='right'caption='[[5lgf~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='5lgf' size='340' side='right'caption='[[5lgf]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lgf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_34438 Atcc 34438~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LGF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lgf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ogataea_polymorpha Ogataea polymorpha]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LGF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgf OCA], [~~http~~://pdbe.org/5lgf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lgf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lgf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgf ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgf OCA], [https://pdbe.org/5lgf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lgf RCSB], [https://www.ebi.ac.uk/pdbsum/5lgf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgf ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/R4IT35_9ASCO R4IT35_9ASCO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 20:

	==See Also==		==See Also==
-	*[[Telomerase\|Telomerase]]	+	*[[Telomerase 3D structures\|Telomerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 34438]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Efimov, S V~~]]	+	[[Category: Ogataea polymorpha]]
-	[[Category: ~~Mantsyzov, A B~~]]	+	[[Category: Efimov SV]]
-	[[Category: ~~Polshakov, V I~~]]	+	[[Category: Mantsyzov AB]]
-	[[Category: ~~Telomerase]]~~	+	[[Category: Polshakov VI]]
-	~~[[Category: Ten domain]]~~	+
-	~~[[Category: Tert]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 13:07, 10 May 2019

OCA — Fri, 10 May 2019 13:07:02 GMT

←Older revision		Revision as of 13:07, 10 May 2019
Line 1:		Line 1:

	==Solution structure of the N-terminal domain of Ogataea polymorpha telomerase reverse transcriptase==		==Solution structure of the N-terminal domain of Ogataea polymorpha telomerase reverse transcriptase==
-	<StructureSection load='5lgf' size='340' side='right' caption='[[5lgf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='5lgf' size='340' side='right'caption='[[5lgf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5lgf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_34438 Atcc 34438]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGF FirstGlance]. <br>		<table><tr><td colspan='2'>[[5lgf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_34438 Atcc 34438]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGF FirstGlance]. <br>
Line 15:		Line 15:
	</div>		</div>
	<div class="pdbe-citations 5lgf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lgf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Telomerase\|Telomerase]]
	== References ==		== References ==
	<references/>		<references/>
Line 20:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 34438]]		[[Category: Atcc 34438]]
		+	[[Category: Large Structures]]
	[[Category: Efimov, S V]]		[[Category: Efimov, S V]]
	[[Category: Mantsyzov, A B]]		[[Category: Mantsyzov, A B]]

OCA at 08:09, 17 January 2018

OCA — Wed, 17 Jan 2018 08:09:24 GMT

←Older revision		Revision as of 08:09, 17 January 2018
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	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	~~Telomerase is a ribonucleoprotein enzyme that adds telomeric~~ DNA ~~fragments to~~ the ends of chromosomes~~. This enzyme~~ is ~~the focus~~ of ~~substantial attention~~, ~~both because~~ its ~~structure and mechanism of action are still poorly studied, and because of its pivotal roles in aging~~ and ~~cellular proliferation~~. ~~The use of telomerase as a potential target for~~ the ~~design~~ of ~~new anticancer drugs is also of great interest. The~~ catalytic ~~protein~~ subunit ~~of telomerase~~ (TERT) ~~contains an~~ N-terminal ~~domain~~ (TEN) ~~that is essential for activity and processivity. Elucidation~~ of the ~~structure~~ and ~~dynamics~~ of TEN in ~~solution is important for understanding~~ the ~~molecular mechanism of~~ telomerase ~~activity~~ and ~~for~~ the ~~design~~ of ~~new telomerase inhibitors. To approach this problem, in this study we report~~ the ~~(1)H, (13)C, and (15)N chemical shift assignments~~ of ~~TEN from Ogataea polymorpha~~. ~~Analysis of~~ the ~~assigned chemical shifts allowed us to identify secondary structures~~ and ~~protein regions potentially~~ involved in ~~interaction with other participants~~ of the ~~telomerase catalytic cycle~~.	+	The elongation of single-stranded DNA repeats at the 3'-ends of chromosomes by telomerase is a key process in maintaining genome integrity in eukaryotes. Abnormal activation of telomerase leads to uncontrolled cell division, whereas its down-regulation is attributed to ageing and several pathologies related to early cell death. Telomerase function is based on the dynamic interactions of its catalytic subunit (TERT) with nucleic acids-telomerase RNA, telomeric DNA and the DNA/RNA heteroduplex. Here, we present the crystallographic and NMR structures of the N-terminal (TEN) domain of TERT from the thermotolerant yeast Hansenula polymorpha and demonstrate the structural conservation of the core motif in evolutionarily divergent organisms. We identify the TEN residues that are involved in interactions with the telomerase RNA and in the recognition of the 'fork' at the distal end of the DNA product/RNA template heteroduplex. We propose that the TEN domain assists telomerase biological function and is involved in restricting the size of the heteroduplex during telomere repeat synthesis.

-	~~NMR assignments~~ of the N-terminal domain of ~~Ogataea polymorpha~~ telomerase reverse transcriptase.~~,Polshakov VI~~, Petrova OA, ~~Parfenova YY~~, Efimov SV, Klochkov VV, Zvereva MI, Dontsova OA ~~Biomol NMR Assign~~. ~~2016 Apr;10(1)~~:~~183-7~~. doi: 10.~~1007~~/~~s12104-015-9663-6. Epub, 2015 Dec 31~~. PMID:~~26721464~~<ref>PMID:~~26721464~~</ref>	+	Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase.,Petrova OA, Mantsyzov AB, Rodina EV, Efimov SV, Hackenberg C, Hakanpaa J, Klochkov VV, Lebedev AA, Chugunova AA, Malyavko AN, Zatsepin TS, Mishin AV, Zvereva MI, Lamzin VS, Dontsova OA, Polshakov VI Nucleic Acids Res. 2017 Dec 23. pii: 4774275. doi: 10.1093/nar/gkx1275. PMID:29294091<ref>PMID:29294091</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 08:50, 27 December 2017

OCA — Wed, 27 Dec 2017 08:50:20 GMT

←Older revision		Revision as of 08:50, 27 December 2017
Line 3:		Line 3:
	<StructureSection load='5lgf' size='340' side='right' caption='[[5lgf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='5lgf' size='340' side='right' caption='[[5lgf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lgf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lgf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_34438 Atcc 34438]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgf OCA], [http://pdbe.org/5lgf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgf RCSB], [http://www.ebi.ac.uk/pdbsum/5lgf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgf ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgf OCA], [http://pdbe.org/5lgf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgf RCSB], [http://www.ebi.ac.uk/pdbsum/5lgf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgf ProSAT]</span></td></tr>
	</table>		</table>
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 34438]]
	[[Category: Efimov, S V]]		[[Category: Efimov, S V]]
	[[Category: Mantsyzov, A B]]		[[Category: Mantsyzov, A B]]

OCA at 05:59, 16 November 2017

OCA — Thu, 16 Nov 2017 05:59:54 GMT

←Older revision		Revision as of 05:59, 16 November 2017
Line 15:		Line 15:
	</div>		</div>
	<div class="pdbe-citations 5lgf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lgf" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Reverse transcriptase\|Reverse transcriptase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:04, 20 October 2017

OCA — Fri, 20 Oct 2017 16:04:39 GMT

←Older revision		Revision as of 16:04, 20 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5lgf is ~~ON HOLD~~	+	==Solution structure of the N-terminal domain of Ogataea polymorpha telomerase reverse transcriptase==
		+	<StructureSection load='5lgf' size='340' side='right' caption='[[5lgf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lgf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGF FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgf OCA], [http://pdbe.org/5lgf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgf RCSB], [http://www.ebi.ac.uk/pdbsum/5lgf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgf ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Telomerase is a ribonucleoprotein enzyme that adds telomeric DNA fragments to the ends of chromosomes. This enzyme is the focus of substantial attention, both because its structure and mechanism of action are still poorly studied, and because of its pivotal roles in aging and cellular proliferation. The use of telomerase as a potential target for the design of new anticancer drugs is also of great interest. The catalytic protein subunit of telomerase (TERT) contains an N-terminal domain (TEN) that is essential for activity and processivity. Elucidation of the structure and dynamics of TEN in solution is important for understanding the molecular mechanism of telomerase activity and for the design of new telomerase inhibitors. To approach this problem, in this study we report the (1)H, (13)C, and (15)N chemical shift assignments of TEN from Ogataea polymorpha. Analysis of the assigned chemical shifts allowed us to identify secondary structures and protein regions potentially involved in interaction with other participants of the telomerase catalytic cycle.

-	~~Authors: Polshakov, V.I~~., ~~Mantsyzov~~, ~~A.B.~~, Efimov, S.V.	+	NMR assignments of the N-terminal domain of Ogataea polymorpha telomerase reverse transcriptase.,Polshakov VI, Petrova OA, Parfenova YY, Efimov SV, Klochkov VV, Zvereva MI, Dontsova OA Biomol NMR Assign. 2016 Apr;10(1):183-7. doi: 10.1007/s12104-015-9663-6. Epub, 2015 Dec 31. PMID:26721464<ref>PMID:26721464</ref>

-	~~Description: Solution structure~~ of the ~~N-terminal domain~~ of ~~Ogataea polymorpha telomerase reverse~~ transcriptase	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Mantsyzov, A.B]]	+	<div class="pdbe-citations 5lgf" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Efimov~~, S.V]]	+
-	[[Category: ~~Polshakov, V.I~~]]	+	==See Also==
		+	*[[Reverse transcriptase\|Reverse transcriptase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Efimov, S V]]
		+	[[Category: Mantsyzov, A B]]
		+	[[Category: Polshakov, V I]]
		+	[[Category: Telomerase]]
		+	[[Category: Ten domain]]
		+	[[Category: Tert]]
		+	[[Category: Transcription]]

OCA at 10:39, 9 March 2017

OCA — Thu, 09 Mar 2017 10:39:38 GMT

←Older revision		Revision as of 10:39, 9 March 2017
Line 7:		Line 7:
	Description: Solution structure of the N-terminal domain of Ogataea polymorpha telomerase reverse transcriptase		Description: Solution structure of the N-terminal domain of Ogataea polymorpha telomerase reverse transcriptase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Polshakov, V.I]]
	[[Category: Mantsyzov, A.B]]		[[Category: Mantsyzov, A.B]]
	[[Category: Efimov, S.V]]		[[Category: Efimov, S.V]]
		+	[[Category: Polshakov, V.I]]

OCA: Protected "5lgf" [edit=sysop:move=sysop]

OCA — Thu, 14 Jul 2016 10:06:41 GMT

Protected "5lgf" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:06, 14 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lgf is ON HOLD Authors: Polshakov, V.I., Mantsyzov, A.B., Efimov, S.V. Description: Solution structure of the N-terminal domain of Ogataea polymor...

OCA — Thu, 14 Jul 2016 10:06:40 GMT

New page: '''Unreleased structure''' The entry 5lgf is ON HOLD Authors: Polshakov, V.I., Mantsyzov, A.B., Efimov, S.V. Description: Solution structure of the N-terminal domain of Ogataea polymor...

New page

'''Unreleased structure'''

The entry 5lgf is ON HOLD

Authors: Polshakov, V.I., Mantsyzov, A.B., Efimov, S.V.

Description: Solution structure of the N-terminal domain of Ogataea polymorpha telomerase reverse transcriptase
[[Category: Unreleased Structures]]
[[Category: Polshakov, V.I]]
[[Category: Mantsyzov, A.B]]
[[Category: Efimov, S.V]]