5lgg - Revision history

OCA at 08:59, 11 October 2023

OCA — Wed, 11 Oct 2023 08:59:17 GMT

←Older revision		Revision as of 08:59, 11 October 2023
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	<StructureSection load='5lgg' size='340' side='right'caption='[[5lgg]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='5lgg' size='340' side='right'caption='[[5lgg]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lgg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LGG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lgg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LGG FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ANAPC1~~, ~~TSG24 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5lgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgg OCA], [~~http~~://pdbe.org/5lgg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lgg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lgg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgg OCA], [https://pdbe.org/5lgg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lgg RCSB], [https://www.ebi.ac.uk/pdbsum/5lgg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/APC1_HUMAN APC1_HUMAN]] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.<ref>PMID:18485873</ref>	+	[https://www.uniprot.org/uniprot/APC1_HUMAN APC1_HUMAN] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.<ref>PMID:18485873</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aibara, S]]	+	[[Category: Aibara S]]
-	[[Category: Barford, D]]	+	[[Category: Barford D]]
-	[[Category: Li, Q]]	+	[[Category: Li Q]]
-	~~[[Category: Apc/c]]~~	+
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Wd40~~]]	+

OCA at 10:36, 12 August 2020

OCA — Wed, 12 Aug 2020 10:36:07 GMT

←Older revision		Revision as of 10:36, 12 August 2020
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	==The N-terminal WD40 domain of Apc1 (Anaphase promoting complex subunit 1)==		==The N-terminal WD40 domain of Apc1 (Anaphase promoting complex subunit 1)==
-	<StructureSection load='5lgg' size='340' side='right' caption='[[5lgg]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='5lgg' size='340' side='right'caption='[[5lgg]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lgg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGG OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LGG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lgg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LGG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgg OCA], [http://pdbe.org/5lgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgg RCSB], [http://www.ebi.ac.uk/pdbsum/5lgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgg ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ANAPC1, TSG24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgg OCA], [http://pdbe.org/5lgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgg RCSB], [http://www.ebi.ac.uk/pdbsum/5lgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Aibara, S]]		[[Category: Aibara, S]]
	[[Category: Barford, D]]		[[Category: Barford, D]]

OCA at 11:02, 26 October 2016

OCA — Wed, 26 Oct 2016 11:02:52 GMT

←Older revision		Revision as of 11:02, 26 October 2016
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgg OCA], [http://pdbe.org/5lgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgg RCSB], [http://www.ebi.ac.uk/pdbsum/5lgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgg ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgg OCA], [http://pdbe.org/5lgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgg RCSB], [http://www.ebi.ac.uk/pdbsum/5lgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/APC1_HUMAN APC1_HUMAN]] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.<ref>PMID:18485873</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 21:37, 5 October 2016

OCA — Wed, 05 Oct 2016 21:37:30 GMT

←Older revision		Revision as of 21:37, 5 October 2016
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-	'''Unreleased structure'''

-	The ~~entry~~ 5lgg is ~~ON HOLD~~	+	==The N-terminal WD40 domain of Apc1 (Anaphase promoting complex subunit 1)==
		+	<StructureSection load='5lgg' size='340' side='right' caption='[[5lgg]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lgg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LGG FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lgg OCA], [http://pdbe.org/5lgg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lgg RCSB], [http://www.ebi.ac.uk/pdbsum/5lgg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lgg ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The anaphase-promoting complex/cyclosome (APC/C) is a large multimeric cullin-RING E3 ubiquitin ligase that orchestrates cell-cycle progression by targeting cell-cycle regulatory proteins for destruction via the ubiquitin proteasome system. The APC/C assembly comprises two scaffolding subcomplexes: the platform and the TPR lobe that together coordinate the juxtaposition of the catalytic and substrate-recognition modules. The platform comprises APC/C subunits Apc1, Apc4, Apc5, and Apc15. Although the role of Apc1 as an APC/C scaffolding subunit has been characterized, its specific functions in contributing toward APC/C catalytic activity are not fully understood. Here, we report the crystal structure of the N-terminal domain of human Apc1 (Apc1N) determined at 2.2-A resolution and provide an atomic-resolution description of the architecture of its WD40 (WD40 repeat) domain (Apc1(WD40)). To understand how Apc1(WD40) contributes to APC/C activity, a mutant form of the APC/C with Apc1(WD40) deleted was generated and evaluated biochemically and structurally. We found that the deletion of Apc1(WD40) abolished the UbcH10-dependent ubiquitination of APC/C substrates without impairing the Ube2S-dependent ubiquitin chain elongation activity. A cryo-EM structure of an APC/C-Cdh1 complex with Apc1(WD40) deleted showed that the mutant APC/C is locked into an inactive conformation in which the UbcH10-binding site of the catalytic module is inaccessible. Additionally, an EM density for Apc15 is not visible. Our data show that Apc1(WD40) is required to mediate the coactivator-induced conformational change of the APC/C that is responsible for stimulating APC/C catalytic activity by promoting UbcH10 binding. In contrast, Ube2S activity toward APC/C substrates is not dependent on the initiation-competent conformation of the APC/C.

-	~~Authors: Li~~, Q., ~~Aibara~~, S., ~~Barford~~, D.	+	WD40 domain of Apc1 is critical for the coactivator-induced allosteric transition that stimulates APC/C catalytic activity.,Li Q, Chang L, Aibara S, Yang J, Zhang Z, Barford D Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10547-52. doi:, 10.1073/pnas.1607147113. Epub 2016 Sep 6. PMID:27601667<ref>PMID:27601667</ref>

-	~~Description: The N-terminal WD40 domain~~ of ~~Apc1 (Anaphase promoting complex subunit 1)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5lgg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Aibara, S]]		[[Category: Aibara, S]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Li, Q]]		[[Category: Li, Q]]
		+	[[Category: Apc/c]]
		+	[[Category: Cell cycle]]
		+	[[Category: Wd40]]

OCA: Protected "5lgg" [edit=sysop:move=sysop]

OCA — Thu, 14 Jul 2016 10:06:47 GMT

Protected "5lgg" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:06, 14 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lgg is ON HOLD Authors: Li, Q., Aibara, S., Barford, D. Description: The N-terminal WD40 domain of Apc1 (Anaphase promoting complex subunit 1) [[C...

OCA — Thu, 14 Jul 2016 10:06:46 GMT

New page: '''Unreleased structure''' The entry 5lgg is ON HOLD Authors: Li, Q., Aibara, S., Barford, D. Description: The N-terminal WD40 domain of Apc1 (Anaphase promoting complex subunit 1) [[C...

New page

'''Unreleased structure'''

The entry 5lgg is ON HOLD

Authors: Li, Q., Aibara, S., Barford, D.

Description: The N-terminal WD40 domain of Apc1 (Anaphase promoting complex subunit 1)
[[Category: Unreleased Structures]]
[[Category: Aibara, S]]
[[Category: Barford, D]]
[[Category: Li, Q]]