5lhk - Revision history

OCA at 18:26, 18 October 2023

OCA — Wed, 18 Oct 2023 18:26:01 GMT

←Older revision		Revision as of 18:26, 18 October 2023
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	<StructureSection load='5lhk' size='340' side='right'caption='[[5lhk]], [[Resolution\|resolution]] 2.32Å' scene=''>		<StructureSection load='5lhk' size='340' side='right'caption='[[5lhk]], [[Resolution\|resolution]] 2.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lhk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_sp.~~_bc16019~~ Streptomyces sp. ~~bc16019~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LHK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lhk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._BC16019 Streptomyces sp. BC16019]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LHK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>~~, ~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.32Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~botP ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=1109705 Streptomyces sp. BC16019]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5lhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lhk OCA], [~~http~~://pdbe.org/5lhk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lhk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lhk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lhk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lhk OCA], [https://pdbe.org/5lhk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lhk RCSB], [https://www.ebi.ac.uk/pdbsum/5lhk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lhk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/K4MHW2_9ACTN K4MHW2_9ACTN]] Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.[SAAS:SAAS00610869]	+	[https://www.uniprot.org/uniprot/K4MHW2_9ACTN K4MHW2_9ACTN] Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.[SAAS:SAAS00610869]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Streptomyces sp. ~~bc16019~~]]	+	[[Category: Streptomyces sp. BC16019]]
-	[[Category: Adam, S]]	+	[[Category: Adam S]]
-	[[Category: Koehnke, J]]	+	[[Category: Koehnke J]]
-	~~[[Category: Botp]]~~	+
-	~~[[Category: Bottromycin]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Peptidase]]~~	+
-	~~[[Category: Ripp~~]]	+

OCA at 10:37, 12 August 2020

OCA — Wed, 12 Aug 2020 10:37:34 GMT

←Older revision		Revision as of 10:37, 12 August 2020
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	==Bottromycin maturation enzyme BotP in complex with Mn==		==Bottromycin maturation enzyme BotP in complex with Mn==
-	<StructureSection load='5lhk' size='340' side='right' caption='[[5lhk]], [[Resolution\|resolution]] 2.32Å' scene=''>	+	<StructureSection load='5lhk' size='340' side='right'caption='[[5lhk]], [[Resolution\|resolution]] 2.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lhk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LHK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lhk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._bc16019 Streptomyces sp. bc16019]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LHK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LHK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lhk OCA], [http://pdbe.org/5lhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lhk RCSB], [http://www.ebi.ac.uk/pdbsum/5lhk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lhk ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1109705 Streptomyces sp. BC16019])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lhk OCA], [http://pdbe.org/5lhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lhk RCSB], [http://www.ebi.ac.uk/pdbsum/5lhk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lhk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5lhk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lhk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aminopeptidase 3D structures\|Aminopeptidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Streptomyces sp. bc16019]]
	[[Category: Adam, S]]		[[Category: Adam, S]]
	[[Category: Koehnke, J]]		[[Category: Koehnke, J]]

OCA at 21:37, 5 October 2016

OCA — Wed, 05 Oct 2016 21:37:37 GMT

←Older revision		Revision as of 21:37, 5 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5lhk is ~~ON HOLD~~	+	==Bottromycin maturation enzyme BotP in complex with Mn==
		+	<StructureSection load='5lhk' size='340' side='right' caption='[[5lhk]], [[Resolution\|resolution]] 2.32Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lhk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LHK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lhk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lhk OCA], [http://pdbe.org/5lhk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lhk RCSB], [http://www.ebi.ac.uk/pdbsum/5lhk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lhk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/K4MHW2_9ACTN K4MHW2_9ACTN]] Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.[SAAS:SAAS00610869]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bottromycins are a family of highly modified peptide natural products displaying potent antimicrobial activity against Gram-positive bacteria including methicillin-resistant Staphyloccoccus aureus. Bottromycins have recently been shown to be ribosomally synthesized and post-translationally modified peptides (RiPPs). Uniquely amongst RiPPs the precursor peptide BotA contains a C-terminal follower, rather than the canonical N- terminal leader sequence. We report the structural and biochemical characterization of BotP, a leucyl-aminopeptidase like enzyme from the bottromycin pathway. We demonstrate that BotP is responsible for the removal of the N-terminal methionine from the precursor peptide. The crystal structures of apo BotP and of BotP in complex with Mn2+ allowed us to model a BotP/substrate complex and to rationalize substrate recognition. Our data represent the first step towards targeted compound modification to unlock the full antibiotic potential of bottromycin.

-	~~Authors~~:	+	Structure and substrate recognition of the Bottromycin maturation enzyme BotP.,Mann G, Huo L, Adam S, Nardone B, Vendome J, Westwood NJ, Muller R, Koehnke J Chembiochem. 2016 Sep 21. doi: 10.1002/cbic.201600406. PMID:27653442<ref>PMID:27653442</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5lhk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Adam, S]]
		+	[[Category: Koehnke, J]]
		+	[[Category: Botp]]
		+	[[Category: Bottromycin]]
		+	[[Category: Hydrolase]]
		+	[[Category: Peptidase]]
		+	[[Category: Ripp]]

OCA: Protected "5lhk" [edit=sysop:move=sysop]

OCA — Tue, 26 Jul 2016 13:31:56 GMT

Protected "5lhk" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:31, 26 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lhk is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Tue, 26 Jul 2016 13:31:55 GMT

New page: '''Unreleased structure''' The entry 5lhk is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5lhk is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]