5lhy - Revision history

OCA at 18:26, 18 October 2023

OCA — Wed, 18 Oct 2023 18:26:36 GMT

←Older revision		Revision as of 18:26, 18 October 2023
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	<StructureSection load='5lhy' size='340' side='right'caption='[[5lhy]], [[Resolution\|resolution]] 3.31Å' scene=''>		<StructureSection load='5lhy' size='340' side='right'caption='[[5lhy]], [[Resolution\|resolution]] 3.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lhy]] is a 60 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LHY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lhy]] is a 60 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LHY FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>PLK4, SAK, STK18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=9606 HUMAN])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.31Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>~~[~~http://en.wikipedia.org/wiki/Polo_kinase Polo kinase~~]~~, with EC number [http://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lhy OCA], [https://pdbe.org/5lhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lhy RCSB], [https://www.ebi.ac.uk/pdbsum/5lhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lhy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5lhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lhy OCA], [~~http~~://pdbe.org/5lhy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lhy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lhy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lhy ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PLK4_HUMAN PLK4_HUMAN]] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2.<ref>PMID:16326102</ref> <ref>PMID:16244668</ref> <ref>PMID:17681131</ref> <ref>PMID:18239451</ref> <ref>PMID:19164942</ref> <ref>PMID:21725316</ref>	+	[https://www.uniprot.org/uniprot/PLK4_HUMAN PLK4_HUMAN] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2.<ref>PMID:16326102</ref> <ref>PMID:16244668</ref> <ref>PMID:17681131</ref> <ref>PMID:18239451</ref> <ref>PMID:19164942</ref> <ref>PMID:21725316</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Polo kinase]]~~	+	[[Category: Cottee MA]]
-	[[Category: Cottee~~, M A~~]]	+	[[Category: Johnson S]]
-	[[Category: Johnson, S]]	+	[[Category: Lea SM]]
-	[[Category: Lea~~, S M]]~~	+
-	~~[[Category: Centriole]]~~	+
-	~~[[Category: Polo box domain]]~~	+
-	~~[[Category: Structural protein]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:38, 12 August 2020

OCA — Wed, 12 Aug 2020 10:38:01 GMT

←Older revision		Revision as of 10:38, 12 August 2020
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==PB3 Domain of Human PLK4 (apo)==		==PB3 Domain of Human PLK4 (apo)==
-	<StructureSection load='5lhy' size='340' side='right' caption='[[5lhy]], [[Resolution\|resolution]] 3.31Å' scene=''>	+	<StructureSection load='5lhy' size='340' side='right'caption='[[5lhy]], [[Resolution\|resolution]] 3.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lhy]] is a 60 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LHY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lhy]] is a 60 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LHY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LHY FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PLK4, SAK, STK18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lhy OCA], [http://pdbe.org/5lhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lhy RCSB], [http://www.ebi.ac.uk/pdbsum/5lhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lhy ProSAT]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lhy OCA], [http://pdbe.org/5lhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lhy RCSB], [http://www.ebi.ac.uk/pdbsum/5lhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lhy ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PLK4_HUMAN PLK4_HUMAN]] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2.<ref>PMID:16326102</ref> <ref>PMID:16244668</ref> <ref>PMID:17681131</ref> <ref>PMID:18239451</ref> <ref>PMID:19164942</ref> <ref>PMID:21725316</ref>		[[http://www.uniprot.org/uniprot/PLK4_HUMAN PLK4_HUMAN]] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2.<ref>PMID:16326102</ref> <ref>PMID:16244668</ref> <ref>PMID:17681131</ref> <ref>PMID:18239451</ref> <ref>PMID:19164942</ref> <ref>PMID:21725316</ref>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5lhy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lhy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Polo kinase]]		[[Category: Polo kinase]]
	[[Category: Cottee, M A]]		[[Category: Cottee, M A]]

OCA at 08:03, 9 March 2017

OCA — Thu, 09 Mar 2017 08:03:07 GMT

←Older revision		Revision as of 08:03, 9 March 2017
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	{{Large structure}}
		+	==PB3 Domain of Human PLK4 (apo)==
		+	<StructureSection load='5lhy' size='340' side='right' caption='[[5lhy]], [[Resolution\|resolution]] 3.31Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lhy]] is a 60 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LHY FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lhy OCA], [http://pdbe.org/5lhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lhy RCSB], [http://www.ebi.ac.uk/pdbsum/5lhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lhy ProSAT]</span></td></tr>
		+	</table>
		+	{{Large structure}}
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PLK4_HUMAN PLK4_HUMAN]] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2.<ref>PMID:16326102</ref> <ref>PMID:16244668</ref> <ref>PMID:17681131</ref> <ref>PMID:18239451</ref> <ref>PMID:19164942</ref> <ref>PMID:21725316</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A small number of proteins form a conserved pathway of centriole duplication. In humans and flies, the binding of Plk4/Sak to STIL/Ana2 initiates daughter centriole assembly. In humans, this interaction is mediated by an interaction between the Polo-Box-3 (PB3) domain of Plk4 and the coiled-coil domain of STIL (HsCCD). We showed previously that the Drosophila Ana2 coiled-coil domain (DmCCD) is essential for centriole assembly, but it forms a tight parallel tetramer in vitro that likely precludes an interaction with PB3. Here we show that the isolated HsCCD and HsPB3 domains form a mixture of homo-multimers in vitro, but these readily dissociate when mixed to form the previously described 1:1 HsCCD:HsPB3 complex. In contrast, although Drosophila PB3 (DmPB3) adopts a canonical polo-box fold, it does not detectably interact with DmCCD in vitro Thus, surprisingly, a key centriole assembly interaction interface appears to differ between humans and flies.

-	~~The entry 5lhy is ON HOLD until Paper Publication~~	+	A key centriole assembly interaction interface between human Plk4 and STIL appears to not be conserved in flies.,Cottee MA, Johnson S, Raff JW, Lea SM Biol Open. 2017 Feb 15. pii: bio.024661. doi: 10.1242/bio.024661. PMID:28202467<ref>PMID:28202467</ref>

-	~~Authors: Cottee~~, ~~M.A~~.~~, Johnson,~~ S.~~, Lea, S.M~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~PB3 Domain of Human PLK4 (apo)~~	+	<div class="pdbe-citations 5lhy" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Polo kinase]]
		+	[[Category: Cottee, M A]]
	[[Category: Johnson, S]]		[[Category: Johnson, S]]
-	[[Category: Lea, S.M]]	+	[[Category: Lea, S M]]
-	[[Category: ~~Cottee, M.A~~]]	+	[[Category: Centriole]]
		+	[[Category: Polo box domain]]
		+	[[Category: Structural protein]]
		+	[[Category: Transferase]]

OCA: Protected "5lhy" [edit=sysop:move=sysop]

OCA — Tue, 26 Jul 2016 13:33:06 GMT

Protected "5lhy" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:33, 26 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lhy is ON HOLD until Paper Publication Authors: Cottee, M.A., Johnson, S., Lea, S.M. Description: PB3 Domain of Human PLK4 (apo) [[Category: Unrel...

OCA — Tue, 26 Jul 2016 13:33:05 GMT

New page: '''Unreleased structure''' The entry 5lhy is ON HOLD until Paper Publication Authors: Cottee, M.A., Johnson, S., Lea, S.M. Description: PB3 Domain of Human PLK4 (apo) [[Category: Unrel...

New page

'''Unreleased structure'''

The entry 5lhy is ON HOLD until Paper Publication

Authors: Cottee, M.A., Johnson, S., Lea, S.M.

Description: PB3 Domain of Human PLK4 (apo)
[[Category: Unreleased Structures]]
[[Category: Johnson, S]]
[[Category: Lea, S.M]]
[[Category: Cottee, M.A]]