5lj6 - Revision history

OCA at 07:25, 1 May 2024

OCA — Wed, 01 May 2024 07:25:22 GMT

←Older revision		Revision as of 07:25, 1 May 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/MACB_AGGAC MACB_AGGAC] Part of the tripartite efflux system MacAB-TdeA. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. Confers resistance against macrolides.<ref>PMID:19432486</ref>		[https://www.uniprot.org/uniprot/MACB_AGGAC MACB_AGGAC] Part of the tripartite efflux system MacAB-TdeA. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. Confers resistance against macrolides.<ref>PMID:19432486</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	MacB is an ABC transporter that collaborates with the MacA adaptor protein and TolC exit duct to drive efflux of antibiotics and enterotoxin STII out of the bacterial cell. Here we present the structure of ATP-bound MacB and reveal precise molecular details of its mechanism. The MacB transmembrane domain lacks a central cavity through which substrates could be passed, but instead conveys conformational changes from one side of the membrane to the other, a process we term mechanotransmission. Comparison of ATP-bound and nucleotide-free states reveals how reversible dimerization of the nucleotide binding domains drives opening and closing of the MacB periplasmic domains via concerted movements of the second transmembrane segment and major coupling helix. We propose that the assembled tripartite pump acts as a molecular bellows to propel substrates through the TolC exit duct, driven by MacB mechanotransmission. Homologs of MacB that do not form tripartite pumps, but share structural features underpinning mechanotransmission, include the LolCDE lipoprotein trafficking complex and FtsEX cell division signaling protein. The MacB architecture serves as the blueprint for understanding the structure and mechanism of an entire ABC transporter superfamily and the many diverse functions it supports.
-
-	Structure and mechanotransmission mechanism of the MacB ABC transporter superfamily.,Crow A, Greene NP, Kaplan E, Koronakis V Proc Natl Acad Sci U S A. 2017 Nov 6. pii: 201712153. doi:, 10.1073/pnas.1712153114. PMID:29109272<ref>PMID:29109272</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5lj6" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:28, 18 October 2023

OCA — Wed, 18 Oct 2023 18:28:53 GMT

←Older revision		Revision as of 18:28, 18 October 2023
Line 1:		Line 1:

	==Structure of Aggregatibacter actinomycetemcomitans MacB bound to ATPyS (P6522)==		==Structure of Aggregatibacter actinomycetemcomitans MacB bound to ATPyS (P6522)==
-	<StructureSection load='5lj6' size='340' side='right' caption='[[5lj6]], [[Resolution\|resolution]] 3.90Å' scene=''>	+	<StructureSection load='5lj6' size='340' side='right'caption='[[5lj6]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lj6]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacterium_acetinomycetum_comitans"_(sic)_(klinger_1912)_colebrook_1920 "bacterium acetinomycetum comitans" (sic) (klinger 1912) colebrook 1920~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJ6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LJ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lj6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aggregatibacter_actinomycetemcomitans Aggregatibacter actinomycetemcomitans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LJ6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=ATP:ADENOSINE~~-~~5-TRIPHOSPHATE'>ATP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~macB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id~~=~~714 "Bacterium acetinomycetum comitans" (sic) (Klinger 1912) Colebrook 1920])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lj6 OCA], [~~http~~://pdbe.org/5lj6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lj6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lj6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lj6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lj6 OCA], [https://pdbe.org/5lj6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lj6 RCSB], [https://www.ebi.ac.uk/pdbsum/5lj6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lj6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MACB_AGGAC MACB_AGGAC]] Part of the tripartite efflux system MacAB-TdeA. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. Confers resistance against macrolides.<ref>PMID:19432486</ref>	+	[https://www.uniprot.org/uniprot/MACB_AGGAC MACB_AGGAC] Part of the tripartite efflux system MacAB-TdeA. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. Confers resistance against macrolides.<ref>PMID:19432486</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Crow, A~~]]	+	[[Category: Aggregatibacter actinomycetemcomitans]]
-	[[Category: ~~Membrane protein abc transporter~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Transport protein~~]]	+	[[Category: Crow A]]

OCA at 07:26, 29 November 2017

OCA — Wed, 29 Nov 2017 07:26:24 GMT

←Older revision		Revision as of 07:26, 29 November 2017
Line 3:		Line 3:
	<StructureSection load='5lj6' size='340' side='right' caption='[[5lj6]], [[Resolution\|resolution]] 3.90Å' scene=''>		<StructureSection load='5lj6' size='340' side='right' caption='[[5lj6]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lj6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LJ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lj6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_acetinomycetum_comitans"_(sic)_(klinger_1912)_colebrook_1920 "bacterium acetinomycetum comitans" (sic) (klinger 1912) colebrook 1920]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LJ6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">macB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=714 "Bacterium acetinomycetum comitans" (sic) (Klinger 1912) Colebrook 1920])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lj6 OCA], [http://pdbe.org/5lj6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lj6 RCSB], [http://www.ebi.ac.uk/pdbsum/5lj6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lj6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lj6 OCA], [http://pdbe.org/5lj6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lj6 RCSB], [http://www.ebi.ac.uk/pdbsum/5lj6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lj6 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:24, 15 November 2017

OCA — Wed, 15 Nov 2017 07:24:14 GMT

←Older revision		Revision as of 07:24, 15 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5lj6 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structure of Aggregatibacter actinomycetemcomitans MacB bound to ATPyS (P6522)==
		+	<StructureSection load='5lj6' size='340' side='right' caption='[[5lj6]], [[Resolution\|resolution]] 3.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lj6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LJ6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lj6 OCA], [http://pdbe.org/5lj6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lj6 RCSB], [http://www.ebi.ac.uk/pdbsum/5lj6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lj6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MACB_AGGAC MACB_AGGAC]] Part of the tripartite efflux system MacAB-TdeA. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. Confers resistance against macrolides.<ref>PMID:19432486</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MacB is an ABC transporter that collaborates with the MacA adaptor protein and TolC exit duct to drive efflux of antibiotics and enterotoxin STII out of the bacterial cell. Here we present the structure of ATP-bound MacB and reveal precise molecular details of its mechanism. The MacB transmembrane domain lacks a central cavity through which substrates could be passed, but instead conveys conformational changes from one side of the membrane to the other, a process we term mechanotransmission. Comparison of ATP-bound and nucleotide-free states reveals how reversible dimerization of the nucleotide binding domains drives opening and closing of the MacB periplasmic domains via concerted movements of the second transmembrane segment and major coupling helix. We propose that the assembled tripartite pump acts as a molecular bellows to propel substrates through the TolC exit duct, driven by MacB mechanotransmission. Homologs of MacB that do not form tripartite pumps, but share structural features underpinning mechanotransmission, include the LolCDE lipoprotein trafficking complex and FtsEX cell division signaling protein. The MacB architecture serves as the blueprint for understanding the structure and mechanism of an entire ABC transporter superfamily and the many diverse functions it supports.

-	~~Authors~~:	+	Structure and mechanotransmission mechanism of the MacB ABC transporter superfamily.,Crow A, Greene NP, Kaplan E, Koronakis V Proc Natl Acad Sci U S A. 2017 Nov 6. pii: 201712153. doi:, 10.1073/pnas.1712153114. PMID:29109272<ref>PMID:29109272</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5lj6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Crow, A]]
		+	[[Category: Membrane protein abc transporter]]
		+	[[Category: Transport protein]]

OCA: Protected "5lj6" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2016 14:00:30 GMT

Protected "5lj6" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:00, 27 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lj6 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Jul 2016 14:00:29 GMT

New page: '''Unreleased structure''' The entry 5lj6 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5lj6 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]