5lj9 - Revision history

OCA at 18:29, 18 October 2023

OCA — Wed, 18 Oct 2023 18:29:03 GMT

←Older revision		Revision as of 18:29, 18 October 2023
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	==Structure of the E. coli MacB ABC domain (C2221)==		==Structure of the E. coli MacB ABC domain (C2221)==
-	<StructureSection load='5lj9' size='340' side='right' caption='[[5lj9]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5lj9' size='340' side='right'caption='[[5lj9]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lj9]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJ9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LJ9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lj9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LJ9 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~macB~~, ~~ybjZ, b0879, JW0863 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=83333 ECOLI])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lj9 OCA], [~~http~~://pdbe.org/5lj9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lj9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lj9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lj9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lj9 OCA], [https://pdbe.org/5lj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lj9 RCSB], [https://www.ebi.ac.uk/pdbsum/5lj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lj9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MACB_ECOLI MACB_ECOLI]] Part of the tripartite efflux system MacAB-TolC. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, the system could also transport R-LPS or a similar glycolipid.<ref>PMID:11544226</ref> <ref>PMID:17214741</ref> <ref>PMID:18955484</ref> <ref>PMID:23974027</ref>	+	[https://www.uniprot.org/uniprot/MACB_ECOLI MACB_ECOLI] Part of the tripartite efflux system MacAB-TolC. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, the system could also transport R-LPS or a similar glycolipid.<ref>PMID:11544226</ref> <ref>PMID:17214741</ref> <ref>PMID:18955484</ref> <ref>PMID:23974027</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
-	[[Category: ~~Crow, A~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Abc transporter]]~~	+	[[Category: Crow A]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 07:26, 29 November 2017

OCA — Wed, 29 Nov 2017 07:26:31 GMT

←Older revision		Revision as of 07:26, 29 November 2017
Line 3:		Line 3:
	<StructureSection load='5lj9' size='340' side='right' caption='[[5lj9]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5lj9' size='340' side='right' caption='[[5lj9]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lj9]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LJ9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lj9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LJ9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lj9 OCA], [http://pdbe.org/5lj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5lj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lj9 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">macB, ybjZ, b0879, JW0863 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lj9 OCA], [http://pdbe.org/5lj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5lj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lj9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Crow, A]]		[[Category: Crow, A]]
	[[Category: Abc transporter]]		[[Category: Abc transporter]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 07:24, 15 November 2017

OCA — Wed, 15 Nov 2017 07:24:27 GMT

←Older revision		Revision as of 07:24, 15 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5lj9 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structure of the E. coli MacB ABC domain (C2221)==
		+	<StructureSection load='5lj9' size='340' side='right' caption='[[5lj9]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lj9]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LJ9 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lj9 OCA], [http://pdbe.org/5lj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5lj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lj9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MACB_ECOLI MACB_ECOLI]] Part of the tripartite efflux system MacAB-TolC. MacB is a non-canonical ABC transporter that contains transmembrane domains (TMD), which form a pore in the inner membrane, and an ATP-binding domain (NBD), which is responsible for energy generation. When overexpressed, the system confers resistance against macrolides composed of 14- and 15-membered lactones but no or weak resistance against 16-membered ones. In addition, the system could also transport R-LPS or a similar glycolipid.<ref>PMID:11544226</ref> <ref>PMID:17214741</ref> <ref>PMID:18955484</ref> <ref>PMID:23974027</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MacB is an ABC transporter that collaborates with the MacA adaptor protein and TolC exit duct to drive efflux of antibiotics and enterotoxin STII out of the bacterial cell. Here we present the structure of ATP-bound MacB and reveal precise molecular details of its mechanism. The MacB transmembrane domain lacks a central cavity through which substrates could be passed, but instead conveys conformational changes from one side of the membrane to the other, a process we term mechanotransmission. Comparison of ATP-bound and nucleotide-free states reveals how reversible dimerization of the nucleotide binding domains drives opening and closing of the MacB periplasmic domains via concerted movements of the second transmembrane segment and major coupling helix. We propose that the assembled tripartite pump acts as a molecular bellows to propel substrates through the TolC exit duct, driven by MacB mechanotransmission. Homologs of MacB that do not form tripartite pumps, but share structural features underpinning mechanotransmission, include the LolCDE lipoprotein trafficking complex and FtsEX cell division signaling protein. The MacB architecture serves as the blueprint for understanding the structure and mechanism of an entire ABC transporter superfamily and the many diverse functions it supports.

-	~~Authors~~:	+	Structure and mechanotransmission mechanism of the MacB ABC transporter superfamily.,Crow A, Greene NP, Kaplan E, Koronakis V Proc Natl Acad Sci U S A. 2017 Nov 6. pii: 201712153. doi:, 10.1073/pnas.1712153114. PMID:29109272<ref>PMID:29109272</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5lj9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Crow, A]]
		+	[[Category: Abc transporter]]
		+	[[Category: Membrane protein]]
		+	[[Category: Transport protein]]

OCA: Protected "5lj9" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2016 14:00:39 GMT

Protected "5lj9" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:00, 27 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lj9 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Jul 2016 14:00:38 GMT

New page: '''Unreleased structure''' The entry 5lj9 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5lj9 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]