5ljp - Revision history

OCA at 18:30, 18 October 2023

OCA — Wed, 18 Oct 2023 18:30:08 GMT

←Older revision		Revision as of 18:30, 18 October 2023
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	<StructureSection load='5ljp' size='340' side='right'caption='[[5ljp]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='5ljp' size='340' side='right'caption='[[5ljp]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ljp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LJP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ljp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7119 Nostoc sp. PCC 7119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LJP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ljp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljp OCA], [~~http~~://pdbe.org/5ljp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ljp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ljp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljp ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ljp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljp OCA], [https://pdbe.org/5ljp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ljp RCSB], [https://www.ebi.ac.uk/pdbsum/5ljp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FLAV_NOSSO FLAV_NOSSO]] Low-potential electron donor to a number of redox enzymes.	+	[https://www.uniprot.org/uniprot/FLAV_NOSSO FLAV_NOSSO] Low-potential electron donor to a number of redox enzymes.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Lamazares, E]]	+	[[Category: Nostoc sp. PCC 7119]]
-	[[Category: Martinez-Julvez, M]]	+	[[Category: Lamazares E]]
-	[[Category: Sancho, J]]	+	[[Category: Martinez-Julvez M]]
-	~~[[Category: Electron transfer]]~~	+	[[Category: Sancho J]]
-	~~[[Category: Electron transport]]~~	+
-	~~[[Category: Flavoprotein~~]]	+

OCA at 10:39, 12 August 2020

OCA — Wed, 12 Aug 2020 10:39:50 GMT

←Older revision		Revision as of 10:39, 12 August 2020
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	==E20K/I59A/E72K/I92A/D126K/A142V FLAVODOXIN FROM ANABAENA==		==E20K/I59A/E72K/I92A/D126K/A142V FLAVODOXIN FROM ANABAENA==
-	<StructureSection load='5ljp' size='340' side='right' caption='[[5ljp]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='5ljp' size='340' side='right'caption='[[5ljp]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ljp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJP OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LJP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ljp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LJP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ljp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljp OCA], [http://pdbe.org/5ljp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ljp RCSB], [http://www.ebi.ac.uk/pdbsum/5ljp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ljp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljp OCA], [http://pdbe.org/5ljp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ljp RCSB], [http://www.ebi.ac.uk/pdbsum/5ljp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5ljp" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5ljp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Flavodoxin 3D structures\|Flavodoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lamazares, E]]		[[Category: Lamazares, E]]
	[[Category: Martinez-Julvez, M]]		[[Category: Martinez-Julvez, M]]

OCA at 03:59, 4 August 2017

OCA — Fri, 04 Aug 2017 03:59:47 GMT

←Older revision		Revision as of 03:59, 4 August 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5ljp is ~~ON HOLD~~	+	==E20K/I59A/E72K/I92A/D126K/A142V FLAVODOXIN FROM ANABAENA==
		+	<StructureSection load='5ljp' size='340' side='right' caption='[[5ljp]], [[Resolution\|resolution]] 1.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ljp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LJP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ljp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljp OCA], [http://pdbe.org/5ljp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ljp RCSB], [http://www.ebi.ac.uk/pdbsum/5ljp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljp ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FLAV_NOSSO FLAV_NOSSO]] Low-potential electron donor to a number of redox enzymes.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Near the minimum free energy basin of proteins where the native ensemble resides, partly unfolded conformations of slightly higher energy can be significantly populated under native conditions. It has been speculated that they play roles in molecular recognition and catalysis, but they might represent contemporary features of the evolutionary process without functional relevance. Obtaining conclusive evidence on these alternatives is difficult because it requires comparing the performance of a given protein when populating and when not populating one such intermediate, in otherwise identical conditions. Wild type apoflavodoxin populates under native conditions a partly unfolded conformation (10% of molecules) whose unstructured region includes the binding sites for the FMN cofactor and for redox partner proteins. We recently engineered a thermostable variant where the intermediate is no longer detectable. Using the wild type and variant, we assess the relevance of the intermediate comparing folding kinetics, cofactor binding kinetics, cofactor affinity, X-ray structure, intrinsic dynamics, redox potential of the apoflavodoxin-cofactor complex (Fld), its affinity for partner protein FNR, and electron transfer rate within the Fld/FNR physiological complex. Our data strongly suggest the intermediate state, conserved in long-chain apoflavodoxins, is not required for the correct assembly of flavodoxin nor does it contribute to shape its electron transfer properties. This analysis can be applied to evaluate other native basin intermediates.

-	~~Authors:~~ Martinez-Julvez, M., ~~Sancho~~, J.~~, Lamazares, E~~.	+	Direct examination of the relevance for folding, binding and electron transfer of a conserved protein folding intermediate.,Lamazares E, Vega S, Ferreira P, Medina M, Galano-Frutos JJ, Martinez-Julvez M, Velazquez-Campoy A, Sancho J Phys Chem Chem Phys. 2017 Jul 26;19(29):19021-19031. doi: 10.1039/c7cp02606d. PMID:28702545<ref>PMID:28702545</ref>

-	~~Description: E20K~~/~~I59A/E72K~~/~~I92A/D126K/A142V FLAVODOXIN FROM ANABAENA~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Sancho, J]]~~	+	<div class="pdbe-citations 5ljp" style="background-color:#fffaf0;"></div>
-	~~[[Category: Martinez-Julvez, M]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Lamazares, E]]		[[Category: Lamazares, E]]
		+	[[Category: Martinez-Julvez, M]]
		+	[[Category: Sancho, J]]
		+	[[Category: Electron transfer]]
		+	[[Category: Electron transport]]
		+	[[Category: Flavoprotein]]

OCA at 10:41, 9 March 2017

OCA — Thu, 09 Mar 2017 10:41:55 GMT

←Older revision		Revision as of 10:41, 9 March 2017
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Sancho, J]]		[[Category: Sancho, J]]
-	[[Category: Lamazares, E]]
	[[Category: Martinez-Julvez, M]]		[[Category: Martinez-Julvez, M]]
		+	[[Category: Lamazares, E]]

OCA: Protected "5ljp" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2016 14:01:41 GMT

Protected "5ljp" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:01, 27 July 2016

OCA: New page: '''Unreleased structure''' The entry 5ljp is ON HOLD Authors: Martinez-Julvez, M., Sancho, J., Lamazares, E. Description: E20K/I59A/E72K/I92A/D126K/A142V FLAVODOXIN FROM ANABAENA [[Cat...

OCA — Wed, 27 Jul 2016 14:01:40 GMT

New page: '''Unreleased structure''' The entry 5ljp is ON HOLD Authors: Martinez-Julvez, M., Sancho, J., Lamazares, E. Description: E20K/I59A/E72K/I92A/D126K/A142V FLAVODOXIN FROM ANABAENA [[Cat...

New page

'''Unreleased structure'''

The entry 5ljp is ON HOLD

Authors: Martinez-Julvez, M., Sancho, J., Lamazares, E.

Description: E20K/I59A/E72K/I92A/D126K/A142V FLAVODOXIN FROM ANABAENA
[[Category: Unreleased Structures]]
[[Category: Sancho, J]]
[[Category: Lamazares, E]]
[[Category: Martinez-Julvez, M]]