5ljx - Revision history

OCA at 18:30, 18 October 2023

OCA — Wed, 18 Oct 2023 18:30:30 GMT

←Older revision		Revision as of 18:30, 18 October 2023
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	<StructureSection load='5ljx' size='340' side='right'caption='[[5ljx]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='5ljx' size='340' side='right'caption='[[5ljx]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ljx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ljx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hantaan_orthohantavirus Hantaan orthohantavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LJX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:~~POTASSIUM+ION~~'>K</scene>, <scene name='pdbligand=NA:~~SODIUM~~+ION'>NA</scene>, <scene name='pdbligand=~~FUL~~:~~BETA-L-FUCOSE~~'>~~FUL~~</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ljx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljx OCA], [~~http~~://pdbe.org/5ljx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ljx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ljx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljx ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ljx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljx OCA], [https://pdbe.org/5ljx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ljx RCSB], [https://www.ebi.ac.uk/pdbsum/5ljx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GP_HANTV GP_HANTV]] Glycoprotein N and Glycoprotein C interact with each. other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promote fusion of viral membrane with host endosomal membrane after endocytosis of the virion. Glycoprotein N contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis (By similarity).<ref>PMID:11886265</ref>	+	[https://www.uniprot.org/uniprot/GP_HANTV GP_HANTV] Glycoprotein N and Glycoprotein C interact with each. other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promote fusion of viral membrane with host endosomal membrane after endocytosis of the virion. Glycoprotein N contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis (By similarity).<ref>PMID:11886265</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hantaan orthohantavirus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Guardado-Calvo, P]]	+	[[Category: Guardado-Calvo P]]
-	[[Category: Rey~~, F A]]~~	+	[[Category: Rey FA]]
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Hantavirus]]~~	+
-	~~[[Category: Viral fusion]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 10:40, 12 August 2020

OCA — Wed, 12 Aug 2020 10:40:05 GMT

←Older revision		Revision as of 10:40, 12 August 2020
Line 1:		Line 1:

	==Structure of hantavirus envelope glycoprotein Gc in postfusion conformation in presence of 600 mM KCL==		==Structure of hantavirus envelope glycoprotein Gc in postfusion conformation in presence of 600 mM KCL==
-	<StructureSection load='5ljx' size='340' side='right' caption='[[5ljx]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='5ljx' size='340' side='right'caption='[[5ljx]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ljx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ljx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LJX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~FUL~~:~~BETA-L-FUCOSE~~'>~~FUL~~</scene>, <scene name='pdbligand=K:~~POTASSIUM~~+ION'>K</scene>, <scene name='pdbligand=NA:~~SODIUM+ION~~'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ljx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljx OCA], [http://pdbe.org/5ljx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ljx RCSB], [http://www.ebi.ac.uk/pdbsum/5ljx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ljx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljx OCA], [http://pdbe.org/5ljx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ljx RCSB], [http://www.ebi.ac.uk/pdbsum/5ljx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Guardado-Calvo, P]]		[[Category: Guardado-Calvo, P]]
	[[Category: Rey, F A]]		[[Category: Rey, F A]]

OCA at 20:08, 9 December 2016

OCA — Fri, 09 Dec 2016 20:08:49 GMT

←Older revision		Revision as of 20:08, 9 December 2016
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/GP_HANTV GP_HANTV]] Glycoprotein N and Glycoprotein C interact with each. other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promote fusion of viral membrane with host endosomal membrane after endocytosis of the virion. Glycoprotein N contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis (By similarity).<ref>PMID:11886265</ref>		[[http://www.uniprot.org/uniprot/GP_HANTV GP_HANTV]] Glycoprotein N and Glycoprotein C interact with each. other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promote fusion of viral membrane with host endosomal membrane after endocytosis of the virion. Glycoprotein N contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis (By similarity).<ref>PMID:11886265</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hantaviruses are zoonotic viruses transmitted to humans by persistently infected rodents, giving rise to serious outbreaks of hemorrhagic fever with renal syndrome (HFRS) or of hantavirus pulmonary syndrome (HPS), depending on the virus, which are associated with high case fatality rates. There is only limited knowledge about the organization of the viral particles and in particular, about the hantavirus membrane fusion glycoprotein Gc, the function of which is essential for virus entry. We describe here the X-ray structures of Gc from Hantaan virus, the type species hantavirus and responsible for HFRS, both in its neutral pH, monomeric pre-fusion conformation, and in its acidic pH, trimeric post-fusion form. The structures confirm the prediction that Gc is a class II fusion protein, containing the characteristic beta-sheet rich domains termed I, II and III as initially identified in the fusion proteins of arboviruses such as alpha- and flaviviruses. The structures also show a number of features of Gc that are distinct from arbovirus class II proteins. In particular, hantavirus Gc inserts residues from three different loops into the target membrane to drive fusion, as confirmed functionally by structure-guided mutagenesis on the HPS-inducing Andes virus, instead of having a single "fusion loop". We further show that the membrane interacting region of Gc becomes structured only at acidic pH via a set of polar and electrostatic interactions. Furthermore, the structure reveals that hantavirus Gc has an additional N-terminal "tail" that is crucial in stabilizing the post-fusion trimer, accompanying the swapping of domain III in the quaternary arrangement of the trimer as compared to the standard class II fusion proteins. The mechanistic understandings derived from these data are likely to provide a unique handle for devising treatments against these human pathogens.
		+
		+	Mechanistic Insight into Bunyavirus-Induced Membrane Fusion from Structure-Function Analyses of the Hantavirus Envelope Glycoprotein Gc.,Guardado-Calvo P, Bignon EA, Stettner E, Jeffers SA, Perez-Vargas J, Pehau-Arnaudet G, Tortorici MA, Jestin JL, England P, Tischler ND, Rey FA PLoS Pathog. 2016 Oct 26;12(10):e1005813. doi: 10.1371/journal.ppat.1005813., eCollection 2016 Oct. PMID:27783711<ref>PMID:27783711</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ljx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:36, 14 September 2016

OCA — Wed, 14 Sep 2016 15:36:36 GMT

←Older revision		Revision as of 15:36, 14 September 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5ljx is ON HOLD until Jul 20 2018~~	+	==Structure of hantavirus envelope glycoprotein Gc in postfusion conformation in presence of 600 mM KCL==
-		+	<StructureSection load='5ljx' size='340' side='right' caption='[[5ljx]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~Authors: Guardado-Calvo, P., Rey, F.A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ljx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LJX FirstGlance]. <br>
-	~~Description:~~ Structure of hantavirus envelope glycoprotein Gc in postfusion conformation in presence of 600 mM KCL	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ljx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ljx OCA], [http://pdbe.org/5ljx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ljx RCSB], [http://www.ebi.ac.uk/pdbsum/5ljx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ljx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GP_HANTV GP_HANTV]] Glycoprotein N and Glycoprotein C interact with each. other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promote fusion of viral membrane with host endosomal membrane after endocytosis of the virion. Glycoprotein N contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis (By similarity).<ref>PMID:11886265</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Guardado-Calvo, P]]		[[Category: Guardado-Calvo, P]]
-	[[Category: Rey, F.A]]	+	[[Category: Rey, F A]]
		+	[[Category: Glycoprotein]]
		+	[[Category: Hantavirus]]
		+	[[Category: Viral fusion]]
		+	[[Category: Viral protein]]

OCA: Protected "5ljx" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2016 14:02:26 GMT

Protected "5ljx" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:02, 27 July 2016

OCA: New page: '''Unreleased structure''' The entry 5ljx is ON HOLD until Jul 20 2018 Authors: Guardado-Calvo, P., Rey, F.A. Description: Structure of hantavirus envelope glycoprotein Gc in postfusio...

OCA — Wed, 27 Jul 2016 14:02:24 GMT

New page: '''Unreleased structure''' The entry 5ljx is ON HOLD until Jul 20 2018 Authors: Guardado-Calvo, P., Rey, F.A. Description: Structure of hantavirus envelope glycoprotein Gc in postfusio...

New page

'''Unreleased structure'''

The entry 5ljx is ON HOLD until Jul 20 2018

Authors: Guardado-Calvo, P., Rey, F.A.

Description: Structure of hantavirus envelope glycoprotein Gc in postfusion conformation in presence of 600 mM KCL
[[Category: Unreleased Structures]]
[[Category: Guardado-Calvo, P]]
[[Category: Rey, F.A]]