5lkb - Revision history

OCA at 18:31, 18 October 2023

OCA — Wed, 18 Oct 2023 18:31:35 GMT

←Older revision		Revision as of 18:31, 18 October 2023
Line 3:		Line 3:
	<StructureSection load='5lkb' size='340' side='right'caption='[[5lkb]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='5lkb' size='340' side='right'caption='[[5lkb]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lkb]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LKB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lkb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LKB FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ECM4, GTO2, YKR076W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=559292 Baker~~'~~s yeast])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5lkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkb OCA], [~~http~~://pdbe.org/5lkb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lkb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lkb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkb OCA], [https://pdbe.org/5lkb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lkb RCSB], [https://www.ebi.ac.uk/pdbsum/5lkb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GTO2_YEAST GTO2_YEAST]] Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB). May be involved in cell wall organization and biogenesis.<ref>PMID:16709151</ref>	+	[https://www.uniprot.org/uniprot/GTO2_YEAST GTO2_YEAST] Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB). May be involved in cell wall organization and biogenesis.<ref>PMID:16709151</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5lkb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lkb" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Didierjean, C]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Favier, F]]	+	[[Category: Didierjean C]]
-	[[Category: Gelhaye, E]]	+	[[Category: Favier F]]
-	[[Category: Girardet~~, J M~~]]	+	[[Category: Gelhaye E]]
-	[[Category: Hecker, A]]	+	[[Category: Girardet JM]]
-	[[Category: Morel-Rouhier, M]]	+	[[Category: Hecker A]]
-	[[Category: Schwartz, M]]	+	[[Category: Morel-Rouhier M]]
-	~~[[Category: Ecm4]]~~	+	[[Category: Schwartz M]]
-	~~[[Category: Glutathione]]~~	+
-	~~[[Category: Glutathione transferase]]~~	+
-	~~[[Category: Glutathionyl-hydroquinone reductase]]~~	+
-	~~[[Category: Quinone]]~~	+
-	~~[[Category: Saccharomyces cerevisiae]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Ykr076w~~]]	+

OCA at 10:40, 12 August 2020

OCA — Wed, 12 Aug 2020 10:40:35 GMT

←Older revision		Revision as of 10:40, 12 August 2020
Line 1:		Line 1:

	==Crystal structure of the Xi glutathione transferase ECM4 from Saccharomyces cerevisiae==		==Crystal structure of the Xi glutathione transferase ECM4 from Saccharomyces cerevisiae==
-	<StructureSection load='5lkb' size='340' side='right' caption='[[5lkb]], [[Resolution\|resolution]] 1.45Å' scene=''>	+	<StructureSection load='5lkb' size='340' side='right'caption='[[5lkb]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lkb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKB OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LKB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lkb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LKB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkb OCA], [http://pdbe.org/5lkb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lkb RCSB], [http://www.ebi.ac.uk/pdbsum/5lkb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkb ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECM4, GTO2, YKR076W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkb OCA], [http://pdbe.org/5lkb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lkb RCSB], [http://www.ebi.ac.uk/pdbsum/5lkb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Didierjean, C]]		[[Category: Didierjean, C]]
	[[Category: Favier, F]]		[[Category: Favier, F]]

OCA at 18:37, 26 October 2016

OCA — Wed, 26 Oct 2016 18:37:49 GMT

←Older revision		Revision as of 18:37, 26 October 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5lkb is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of the Xi glutathione transferase ECM4 from Saccharomyces cerevisiae==
		+	<StructureSection load='5lkb' size='340' side='right' caption='[[5lkb]], [[Resolution\|resolution]] 1.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lkb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LKB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkb OCA], [http://pdbe.org/5lkb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lkb RCSB], [http://www.ebi.ac.uk/pdbsum/5lkb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GTO2_YEAST GTO2_YEAST]] Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB). May be involved in cell wall organization and biogenesis.<ref>PMID:16709151</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glutathionyl-hydroquinone reductases (GHRs) belong to the recently characterized Xi-class of glutathione transferases (GSTXs) according to unique structural properties and are present in all but animal kingdoms. The GHR ScECM4 from the yeast Saccharomyces cerevisiae has been studied since 1997 when it was found to be potentially involved in cell-wall biosynthesis. Up to now and in spite of biological studies made on this enzyme, its physiological role remains challenging. The work here reports its crystallographic study. In addition to exhibiting the general GSTX structural features, ScECM4 shows extensions including a huge loop which contributes to the quaternary assembly. These structural extensions are probably specific to Saccharomycetaceae. Soaking of ScECM4 crystals with GS-menadione results in a structure where glutathione forms a mixed disulfide bond with the cysteine 46. Solution studies confirm that ScECM4 has reductase activity for GS-menadione in presence of glutathione. Moreover, the high resolution structures allowed us to propose new roles of conserved residues of the active site to assist the cysteine 46 during the catalytic act.

-	~~Authors: Schwartz~~, M., ~~Didierjean~~, C., Hecker, A., Girardet~~, J.M.~~, Morel-Rouhier, M., Gelhaye, E., Favier, F.	+	Crystal Structure of Saccharomyces cerevisiae ECM4, a Xi-Class Glutathione Transferase that Reacts with Glutathionyl-(hydro)quinones.,Schwartz M, Didierjean C, Hecker A, Girardet JM, Morel-Rouhier M, Gelhaye E, Favier F PLoS One. 2016 Oct 13;11(10):e0164678. doi: 10.1371/journal.pone.0164678., eCollection 2016. PMID:27736955<ref>PMID:27736955</ref>

-	~~Description: Crystal structure~~ of the ~~Xi glutathione transferase ECM4 from Saccharomyces cerevisiae~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Girardet, J.M]]~~	+	<div class="pdbe-citations 5lkb" style="background-color:#fffaf0;"></div>
-	~~[[Category: Morel-Rouhier, M]]~~	+	== References ==
-	~~[[Category: Schwartz, M]]~~	+	<references/>
-	~~[[Category: Hecker, A]]~~	+	__TOC__
-	~~[[Category: Favier, F]]~~	+	</StructureSection>
	[[Category: Didierjean, C]]		[[Category: Didierjean, C]]
		+	[[Category: Favier, F]]
	[[Category: Gelhaye, E]]		[[Category: Gelhaye, E]]
		+	[[Category: Girardet, J M]]
		+	[[Category: Hecker, A]]
		+	[[Category: Morel-Rouhier, M]]
		+	[[Category: Schwartz, M]]
		+	[[Category: Ecm4]]
		+	[[Category: Glutathione]]
		+	[[Category: Glutathione transferase]]
		+	[[Category: Glutathionyl-hydroquinone reductase]]
		+	[[Category: Quinone]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Transferase]]
		+	[[Category: Ykr076w]]

OCA at 02:54, 4 August 2016

OCA — Thu, 04 Aug 2016 02:54:13 GMT

←Older revision		Revision as of 02:54, 4 August 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5lkb is ON HOLD	+	The entry 5lkb is ON HOLD until Paper Publication

	Authors: Schwartz, M., Didierjean, C., Hecker, A., Girardet, J.M., Morel-Rouhier, M., Gelhaye, E., Favier, F.		Authors: Schwartz, M., Didierjean, C., Hecker, A., Girardet, J.M., Morel-Rouhier, M., Gelhaye, E., Favier, F.

OCA: Protected "5lkb" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2016 14:03:22 GMT

Protected "5lkb" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:03, 27 July 2016

OCA: New page: '''Unreleased structure''' The entry 5lkb is ON HOLD Authors: Schwartz, M., Didierjean, C., Hecker, A., Girardet, J.M., Morel-Rouhier, M., Gelhaye, E., Favier, F. Description: Crystal ...

OCA — Wed, 27 Jul 2016 14:03:21 GMT

New page: '''Unreleased structure''' The entry 5lkb is ON HOLD Authors: Schwartz, M., Didierjean, C., Hecker, A., Girardet, J.M., Morel-Rouhier, M., Gelhaye, E., Favier, F. Description: Crystal ...

New page

'''Unreleased structure'''

The entry 5lkb is ON HOLD

Authors: Schwartz, M., Didierjean, C., Hecker, A., Girardet, J.M., Morel-Rouhier, M., Gelhaye, E., Favier, F.

Description: Crystal structure of the Xi glutathione transferase ECM4 from Saccharomyces cerevisiae
[[Category: Unreleased Structures]]
[[Category: Girardet, J.M]]
[[Category: Morel-Rouhier, M]]
[[Category: Schwartz, M]]
[[Category: Hecker, A]]
[[Category: Favier, F]]
[[Category: Didierjean, C]]
[[Category: Gelhaye, E]]