5lkw - Revision history

OCA at 18:32, 18 October 2023

OCA — Wed, 18 Oct 2023 18:32:17 GMT

←Older revision		Revision as of 18:32, 18 October 2023
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	<StructureSection load='5lkw' size='340' side='right'caption='[[5lkw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5lkw' size='340' side='right'caption='[[5lkw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lkw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Burcj Burcj~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LKW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lkw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_cenocepacia_J2315 Burkholderia cenocepacia J2315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LKW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=API:~~2~~,6-DIAMINOPIMELIC+ACID'>API</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~BCAL3204 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=216591 BURCJ])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkw OCA], [~~http~~://pdbe.org/5lkw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lkw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lkw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkw OCA], [https://pdbe.org/5lkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lkw RCSB], [https://www.ebi.ac.uk/pdbsum/5lkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B4EDC1_BURCJ B4EDC1_BURCJ]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5lkw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lkw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Pal\|Pal]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Burcj~~]]	+	[[Category: Burkholderia cenocepacia J2315]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Berisio, R]]	+	[[Category: Berisio R]]
-	[[Category: Romano, M]]	+	[[Category: Romano M]]
-	[[Category: Ruggiero, A]]	+	[[Category: Ruggiero A]]
-	~~[[Category: Dap]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Peptidoglycan-associated lipoprotein~~]]	+

OCA at 06:29, 16 October 2019

OCA — Wed, 16 Oct 2019 06:29:44 GMT

←Older revision		Revision as of 06:29, 16 October 2019
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	==Crystal structure of the peptidoglycan-associated lipoprotein (Pal) from Burkholderia cepacia in complex with DAP==		==Crystal structure of the peptidoglycan-associated lipoprotein (Pal) from Burkholderia cepacia in complex with DAP==
-	<StructureSection load='5lkw' size='340' side='right' caption='[[5lkw]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5lkw' size='340' side='right'caption='[[5lkw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5lkw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burcj Burcj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LKW FirstGlance]. <br>		<table><tr><td colspan='2'>[[5lkw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burcj Burcj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LKW FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Burcj]]		[[Category: Burcj]]
		+	[[Category: Large Structures]]
	[[Category: Berisio, R]]		[[Category: Berisio, R]]
	[[Category: Romano, M]]		[[Category: Romano, M]]

OCA at 07:39, 6 December 2017

OCA — Wed, 06 Dec 2017 07:39:39 GMT

←Older revision		Revision as of 07:39, 6 December 2017
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkw OCA], [http://pdbe.org/5lkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lkw RCSB], [http://www.ebi.ac.uk/pdbsum/5lkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkw OCA], [http://pdbe.org/5lkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lkw RCSB], [http://www.ebi.ac.uk/pdbsum/5lkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkw ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Burkholderia cepacia complex (Bcc) is a group of Gram-negative opportunistic pathogens causing infections in people with cystic fibrosis (CF). Bcc is highly antibiotic resistant, making conventional antibiotic treatment problematic. The identification of novel targets for anti-virulence therapies should improve therapeutic options for infected CF patients. We previously identified that the peptidoglycan-associated lipoprotein (Pal) was immunogenic in Bcc infected CF patients; however, its role in Bcc pathogenesis is unknown. The virulence of a pal deletion mutant (Deltapal) in Galleria mellonella was 88-fold reduced (p < .001) compared to wild type. The lipopolysaccharide profiles of wild type and Deltapal were identical, indicating no involvement of Pal in O-antigen transport. However, Deltapal was more susceptible to polymyxin B. Structural elucidation by X-ray crystallography and calorimetry demonstrated that Pal binds peptidoglycan fragments. Deltapal showed a 1.5-fold reduced stimulation of IL-8 in CF epithelial cells relative to wild type (p < .001), demonstrating that Pal is a significant driver of inflammation. The Deltapal mutant had reduced binding to CFBE41o(-) cells, but adhesion of Pal-expressing recombinant E. coli to CFBE41o(-) cells was enhanced compared to wild-type E. coli (p < .0001), confirming that Pal plays a direct role in host cell attachment. Overall, Bcc Pal mediates host cell attachment and stimulation of cytokine secretion, contributing to Bcc pathogenesis.
		+
		+	The Burkholderia cenocepacia peptidoglycan-associated lipoprotein is involved in epithelial cell attachment and elicitation of inflammation.,Dennehy R, Romano M, Ruggiero A, Mohamed YF, Dignam SL, Mujica Troncoso C, Callaghan M, Valvano MA, Berisio R, McClean S Cell Microbiol. 2017 May;19(5). doi: 10.1111/cmi.12691. Epub 2016 Nov 25. PMID:27886433<ref>PMID:27886433</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5lkw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 23:49, 15 November 2017

OCA — Wed, 15 Nov 2017 23:49:49 GMT

←Older revision		Revision as of 23:49, 15 November 2017
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-	'''Unreleased structure'''

-	~~The entry 5lkw is ON HOLD~~	+	==Crystal structure of the peptidoglycan-associated lipoprotein (Pal) from Burkholderia cepacia in complex with DAP==
-		+	<StructureSection load='5lkw' size='340' side='right' caption='[[5lkw]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors: Romano, M., Ruggiero, A., Berisio, R.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5lkw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burcj Burcj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LKW FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the peptidoglycan-associated lipoprotein (Pal) from Burkholderia cepacia in complex with DAP	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=API:2,6-DIAMINOPIMELIC+ACID'>API</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCAL3204 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216591 BURCJ])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lkw OCA], [http://pdbe.org/5lkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lkw RCSB], [http://www.ebi.ac.uk/pdbsum/5lkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lkw ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Burcj]]
		+	[[Category: Berisio, R]]
		+	[[Category: Romano, M]]
	[[Category: Ruggiero, A]]		[[Category: Ruggiero, A]]
-	[[Category: ~~Romano, M~~]]	+	[[Category: Dap]]
-	[[Category: ~~Berisio, R~~]]	+	[[Category: Membrane protein]]
		+	[[Category: Peptidoglycan-associated lipoprotein]]

OCA: Protected "5lkw" [edit=sysop:move=sysop]

OCA — Thu, 04 Aug 2016 02:55:55 GMT

Protected "5lkw" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:55, 4 August 2016

OCA: New page: '''Unreleased structure''' The entry 5lkw is ON HOLD Authors: Romano, M., Ruggiero, A., Berisio, R. Description: Crystal structure of the peptidoglycan-associated lipoprotein (Pal) fro...

OCA — Thu, 04 Aug 2016 02:55:54 GMT

New page: '''Unreleased structure''' The entry 5lkw is ON HOLD Authors: Romano, M., Ruggiero, A., Berisio, R. Description: Crystal structure of the peptidoglycan-associated lipoprotein (Pal) fro...

New page

'''Unreleased structure'''

The entry 5lkw is ON HOLD

Authors: Romano, M., Ruggiero, A., Berisio, R.

Description: Crystal structure of the peptidoglycan-associated lipoprotein (Pal) from Burkholderia cepacia in complex with DAP
[[Category: Unreleased Structures]]
[[Category: Ruggiero, A]]
[[Category: Romano, M]]
[[Category: Berisio, R]]