5ll3 - Revision history

OCA at 18:32, 18 October 2023

OCA — Wed, 18 Oct 2023 18:32:48 GMT

←Older revision		Revision as of 18:32, 18 October 2023
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	<StructureSection load='5ll3' size='340' side='right'caption='[[5ll3]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='5ll3' size='340' side='right'caption='[[5ll3]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ll3]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_buchneri"_(sic)_henneberg_1903 "bacillus~~ buchneri~~" (sic) henneberg 1903~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LL3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LL3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ll3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentilactobacillus_buchneri Lentilactobacillus buchneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LL3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LL3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PLP:PYRIDOXAL~~-~~5-PHOSPHATE'>PLP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Isoleucine_2~~-~~epimerase Isoleucine 2~~-~~epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.21 5.1.1.21]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ll3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ll3 OCA], [~~http~~://pdbe.org/5ll3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ll3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ll3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ll3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ll3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ll3 OCA], [https://pdbe.org/5ll3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ll3 RCSB], [https://www.ebi.ac.uk/pdbsum/5ll3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ll3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ILE2E_LENBU ILE2E_LENBU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Isoleucine 2-epimerase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Awad, R]]	+	[[Category: Lentilactobacillus buchneri]]
-	[[Category: Gans, P]]	+	[[Category: Awad R]]
-	[[Category: Reiser, J B]]	+	[[Category: Gans P]]
-	~~[[Category: Epimerase]]~~	+	[[Category: Reiser J-B]]
-	~~[[Category: Isoleucine]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Lactobacillus buchneri]]~~	+
-	~~[[Category: Plp]]~~	+
-	~~[[Category: Racemase~~]]	+

OCA at 10:41, 12 August 2020

OCA — Wed, 12 Aug 2020 10:41:14 GMT

←Older revision		Revision as of 10:41, 12 August 2020
Line 1:		Line 1:

	==Structure of the Isoleucine 2-epimerase from Lactobacillus buchneri (PLP complex form)==		==Structure of the Isoleucine 2-epimerase from Lactobacillus buchneri (PLP complex form)==
-	<StructureSection load='5ll3' size='340' side='right' caption='[[5ll3]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='5ll3' size='340' side='right'caption='[[5ll3]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ll3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_buchneri"_(sic)_henneberg_1903 "bacillus buchneri" (sic) henneberg 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LL3 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LL3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ll3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_buchneri"_(sic)_henneberg_1903 "bacillus buchneri" (sic) henneberg 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LL3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LL3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isoleucine_2-epimerase Isoleucine 2-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.21 5.1.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isoleucine_2-epimerase Isoleucine 2-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.21 5.1.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ll3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ll3 OCA], [http://pdbe.org/5ll3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ll3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ll3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ll3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ll3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ll3 OCA], [http://pdbe.org/5ll3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ll3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ll3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ll3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Isoleucine 2-epimerase]]		[[Category: Isoleucine 2-epimerase]]
		+	[[Category: Large Structures]]
	[[Category: Awad, R]]		[[Category: Awad, R]]
	[[Category: Gans, P]]		[[Category: Gans, P]]

OCA at 01:20, 16 November 2017

OCA — Thu, 16 Nov 2017 01:20:17 GMT

←Older revision		Revision as of 01:20, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='5ll3' size='340' side='right' caption='[[5ll3]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='5ll3' size='340' side='right' caption='[[5ll3]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ll3]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LL3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LL3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ll3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_buchneri"_(sic)_henneberg_1903 "bacillus buchneri" (sic) henneberg 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LL3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LL3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isoleucine_2-epimerase Isoleucine 2-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.21 5.1.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isoleucine_2-epimerase Isoleucine 2-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.21 5.1.1.21] </span></td></tr>

OCA at 11:40, 12 April 2017

OCA — Wed, 12 Apr 2017 11:40:38 GMT

←Older revision		Revision as of 11:40, 12 April 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ll3 is ~~ON HOLD~~	+	==Structure of the Isoleucine 2-epimerase from Lactobacillus buchneri (PLP complex form)==
		+	<StructureSection load='5ll3' size='340' side='right' caption='[[5ll3]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ll3]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LL3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LL3 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isoleucine_2-epimerase Isoleucine 2-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.21 5.1.1.21] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ll3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ll3 OCA], [http://pdbe.org/5ll3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ll3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ll3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ll3 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The isoleucine 2-epimerase from Lactobacillus buchneri has been previously identified and characterized to catalyze the pyridoxal 5'-phosphate (PLP)-dependent racemization and epimerization of a broad spectrum of nonpolar amino acids from L- to D-form and vice versa, in particular isoleucine. In this study, crystal structures of both native and PLP-complex forms of this racemase are presented at 2.6 and 2.15 A resolution, respectively. Both structures show that the protein belongs to the fold-type I subgroup of PLP-dependent enzymes and is very close to aminobutyrate aminotransferases family, as it has been suspected because of their sequence homology. The extensive structural comparison with fold-type I enzymes with known amino acid racemization activities, including the alpha-amino-epsilon-caprolactam racemase from Achromobacter obae and the cystathionine beta-lyase from Escherichia coli, allows us to identify the active site residues responsible for its nonpolar amino acid recognition and reactivity specificity. Our observations also suggest that the racemization reaction by the fold-type I racemases may generally occur thanks to a revised two-base mechanism. Lastly, both structures reveal details on the conformational changes provoked by PLP binding that suggest an induced fit of the active site "entrance door", necessary to accommodate PLP and substrate molecules.

-	~~Authors: Reiser, J.~~-B., Awad, R., Gans, P.	+	Structural insights into the substrate recognition and reaction specificity of the PLP-dependent fold-type I isoleucine 2-epimerase from Lactobacillus buchneri.,Awad R, Gans P, Reiser JB Biochimie. 2017 Mar 23. pii: S0300-9084(16)30277-2. doi:, 10.1016/j.biochi.2017.03.015. PMID:28344038<ref>PMID:28344038</ref>

-	~~Description: Structure~~ of the ~~Isoleucine 2-epimerase from Lactobacillus buchneri (PLP complex form)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Reiser, J.~~-B]]	+	<div class="pdbe-citations 5ll3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Isoleucine 2-epimerase]]
	[[Category: Awad, R]]		[[Category: Awad, R]]
	[[Category: Gans, P]]		[[Category: Gans, P]]
		+	[[Category: Reiser, J B]]
		+	[[Category: Epimerase]]
		+	[[Category: Isoleucine]]
		+	[[Category: Isomerase]]
		+	[[Category: Lactobacillus buchneri]]
		+	[[Category: Plp]]
		+	[[Category: Racemase]]

OCA at 10:43, 9 March 2017

OCA — Thu, 09 Mar 2017 10:43:07 GMT

←Older revision		Revision as of 10:43, 9 March 2017
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Reiser, J.-B]]		[[Category: Reiser, J.-B]]
-	[[Category: Gans, P]]
	[[Category: Awad, R]]		[[Category: Awad, R]]
		+	[[Category: Gans, P]]

OCA: Protected "5ll3" [edit=sysop:move=sysop]

OCA — Thu, 04 Aug 2016 02:56:27 GMT

Protected "5ll3" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:56, 4 August 2016

OCA: New page: '''Unreleased structure''' The entry 5ll3 is ON HOLD Authors: Reiser, J.-B., Awad, R., Gans, P. Description: Structure of the Isoleucine 2-epimerase from Lactobacillus buchneri (PLP co...

OCA — Thu, 04 Aug 2016 02:56:25 GMT

New page: '''Unreleased structure''' The entry 5ll3 is ON HOLD Authors: Reiser, J.-B., Awad, R., Gans, P. Description: Structure of the Isoleucine 2-epimerase from Lactobacillus buchneri (PLP co...

New page

'''Unreleased structure'''

The entry 5ll3 is ON HOLD

Authors: Reiser, J.-B., Awad, R., Gans, P.

Description: Structure of the Isoleucine 2-epimerase from Lactobacillus buchneri (PLP complex form)
[[Category: Unreleased Structures]]
[[Category: Reiser, J.-B]]
[[Category: Gans, P]]
[[Category: Awad, R]]