5lme - Revision history

OCA at 10:15, 14 June 2023

OCA — Wed, 14 Jun 2023 10:15:15 GMT

←Older revision		Revision as of 10:15, 14 June 2023
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	==Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase==		==Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase==
-	<StructureSection load='5lme' size='340' side='right'caption='[[5lme~~]], [[NMR_Ensembles_of_Models \| 24 NMR models~~]]' scene=''>	+	<StructureSection load='5lme' size='340' side='right'caption='[[5lme]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lme]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LME OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LME FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lme]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoplusia_ni Trichoplusia ni]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LME FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lme OCA], [~~http~~://pdbe.org/5lme PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lme RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lme PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lme ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lme OCA], [https://pdbe.org/5lme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lme RCSB], [https://www.ebi.ac.uk/pdbsum/5lme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lme ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q27026_TRINI Q27026_TRINI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5lme" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lme" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transposase 3D structures\|Transposase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bardiaux, B]]	+	[[Category: Trichoplusia ni]]
-	[[Category: Betermier, M]]	+	[[Category: Bardiaux B]]
-	[[Category: Bischerour, J]]	+	[[Category: Betermier M]]
-	[[Category: Craig~~, N L~~]]	+	[[Category: Bischerour J]]
-	[[Category: Dyda, F]]	+	[[Category: Craig NL]]
-	[[Category: Guittet, E]]	+	[[Category: Dyda F]]
-	[[Category: Hickman~~, A B~~]]	+	[[Category: Guittet E]]
-	[[Category: Lescop, E]]	+	[[Category: Hickman AB]]
-	[[Category: Li, X]]	+	[[Category: Lescop E]]
-	[[Category: Mathy, N]]	+	[[Category: Li X]]
-	[[Category: Morellet, N]]	+	[[Category: Mathy N]]
-	[[Category: Moriau, S]]	+	[[Category: Morellet N]]
-	[[Category: Nilges, M]]	+	[[Category: Moriau S]]
-	[[Category: Taylor~~, J A~~]]	+	[[Category: Nilges M]]
-	[[Category: Wieninger, S]]	+	[[Category: Taylor JA]]
-	~~[[Category: Transcription]]~~	+	[[Category: Wieninger S]]
-	~~[[Category: Transposase piggybac dna-binding zinc-finger~~]]	+

OCA at 13:07, 10 May 2019

OCA — Fri, 10 May 2019 13:07:18 GMT

←Older revision		Revision as of 13:07, 10 May 2019
Line 1:		Line 1:

	==Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase==		==Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase==
-	<StructureSection load='5lme' size='340' side='right' caption='[[5lme]], [[NMR_Ensembles_of_Models \| 24 NMR models]]' scene=''>	+	<StructureSection load='5lme' size='340' side='right'caption='[[5lme]], [[NMR_Ensembles_of_Models \| 24 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5lme]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LME FirstGlance]. <br>		<table><tr><td colspan='2'>[[5lme]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LME FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Bardiaux, B]]		[[Category: Bardiaux, B]]
	[[Category: Betermier, M]]		[[Category: Betermier, M]]

OCA at 06:55, 15 February 2018

OCA — Thu, 15 Feb 2018 06:55:46 GMT

←Older revision		Revision as of 06:55, 15 February 2018
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lme OCA], [http://pdbe.org/5lme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lme RCSB], [http://www.ebi.ac.uk/pdbsum/5lme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lme ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lme OCA], [http://pdbe.org/5lme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lme RCSB], [http://www.ebi.ac.uk/pdbsum/5lme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lme ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The piggyBac transposase (PB) is distinguished by its activity and utility in genome engineering, especially in humans where it has highly promising therapeutic potential. Little is known, however, about the structure-function relationships of the different domains of PB. Here, we demonstrate in vitro and in vivo that its C-terminal Cysteine-Rich Domain (CRD) is essential for DNA breakage, joining and transposition and that it binds to specific DNA sequences in the left and right transposon ends, and to an additional unexpectedly internal site at the left end. Using NMR, we show that the CRD adopts the specific fold of the cross-brace zinc finger protein family. We determine the interaction interfaces between the CRD and its target, the 5'-TGCGT-3'/3'-ACGCA-5' motifs found in the left, left internal and right transposon ends, and use NMR results to propose docking models for the complex, which are consistent with our site-directed mutagenesis data. Our results provide support for a model of the PB/DNA interactions in the context of the transpososome, which will be useful for the rational design of PB mutants with increased activity.
		+
		+	Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase.,Morellet N, Li X, Wieninger SA, Taylor JL, Bischerour J, Moriau S, Lescop E, Bardiaux B, Mathy N, Assrir N, Betermier M, Nilges M, Hickman AB, Dyda F, Craig NL, Guittet E Nucleic Acids Res. 2018 Jan 27. pii: 4827089. doi: 10.1093/nar/gky044. PMID:29385532<ref>PMID:29385532</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5lme" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:03, 20 December 2017

OCA — Wed, 20 Dec 2017 06:03:27 GMT

←Older revision		Revision as of 06:03, 20 December 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5lme is ~~ON HOLD until Paper Publication~~	+	==Specific-DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase==
-		+	<StructureSection load='5lme' size='340' side='right' caption='[[5lme]], [[NMR_Ensembles_of_Models \| 24 NMR models]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5lme]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LME FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lme OCA], [http://pdbe.org/5lme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lme RCSB], [http://www.ebi.ac.uk/pdbsum/5lme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lme ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bardiaux, B]]
		+	[[Category: Betermier, M]]
		+	[[Category: Bischerour, J]]
		+	[[Category: Craig, N L]]
		+	[[Category: Dyda, F]]
		+	[[Category: Guittet, E]]
		+	[[Category: Hickman, A B]]
		+	[[Category: Lescop, E]]
		+	[[Category: Li, X]]
		+	[[Category: Mathy, N]]
		+	[[Category: Morellet, N]]
		+	[[Category: Moriau, S]]
		+	[[Category: Nilges, M]]
		+	[[Category: Taylor, J A]]
		+	[[Category: Wieninger, S]]
		+	[[Category: Transcription]]
		+	[[Category: Transposase piggybac dna-binding zinc-finger]]

OCA at 10:51, 27 September 2017

OCA — Wed, 27 Sep 2017 10:51:07 GMT

←Older revision		Revision as of 10:51, 27 September 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5lme is ON HOLD	+	The entry 5lme is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5lme" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2016 14:38:45 GMT

Protected "5lme" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:38, 10 August 2016

OCA: New page: '''Unreleased structure''' The entry 5lme is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 10 Aug 2016 14:38:44 GMT

New page: '''Unreleased structure''' The entry 5lme is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5lme is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]