5lt0 - Revision history

OCA at 18:47, 18 October 2023

OCA — Wed, 18 Oct 2023 18:47:33 GMT

←Older revision		Revision as of 18:47, 18 October 2023
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	<StructureSection load='5lt0' size='340' side='right'caption='[[5lt0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5lt0' size='340' side='right'caption='[[5lt0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lt0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LT0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lt0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LT0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5lt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lt0 OCA], [~~http~~://pdbe.org/5lt0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lt0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lt0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lt0 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lt0 OCA], [https://pdbe.org/5lt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lt0 RCSB], [https://www.ebi.ac.uk/pdbsum/5lt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lt0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/KINH_HUMAN KINH_HUMAN] Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cao, L]]	+	[[Category: Cao L]]
-	[[Category: Gigant, B]]	+	[[Category: Gigant B]]
-	~~[[Category: Adp dissociation]]~~	+
-	~~[[Category: Kinesin motor domain]]~~	+
-	~~[[Category: Motor protein]]~~	+
-	~~[[Category: Nucleotide-free~~]]	+

OCA at 10:49, 12 August 2020

OCA — Wed, 12 Aug 2020 10:49:18 GMT

←Older revision		Revision as of 10:49, 12 August 2020
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	==nucleotide-free kinesin-1 motor domain, P212121 crystal form==		==nucleotide-free kinesin-1 motor domain, P212121 crystal form==
-	<StructureSection load='5lt0' size='340' side='right' caption='[[5lt0]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5lt0' size='340' side='right'caption='[[5lt0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lt0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LT0 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lt0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LT0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LT0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lt0 OCA], [http://pdbe.org/5lt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lt0 RCSB], [http://www.ebi.ac.uk/pdbsum/5lt0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lt0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lt0 OCA], [http://pdbe.org/5lt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lt0 RCSB], [http://www.ebi.ac.uk/pdbsum/5lt0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lt0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5lt0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lt0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Kinesin 3D Structures\|Kinesin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Cao, L]]		[[Category: Cao, L]]
	[[Category: Gigant, B]]		[[Category: Gigant, B]]

OCA at 09:37, 11 March 2017

OCA — Sat, 11 Mar 2017 09:37:10 GMT

←Older revision		Revision as of 09:37, 11 March 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5lt0 is ~~ON HOLD until Paper~~ Publication	+	==nucleotide-free kinesin-1 motor domain, P212121 crystal form==
		+	<StructureSection load='5lt0' size='340' side='right' caption='[[5lt0]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lt0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LT0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lt0 OCA], [http://pdbe.org/5lt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lt0 RCSB], [http://www.ebi.ac.uk/pdbsum/5lt0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lt0 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kinesin-1 is an ATP-dependent motor protein that moves towards microtubules (+)-ends. Whereas structures of isolated ADP-kinesin and of complexes with tubulin of apo-kinesin and of ATP-like-kinesin are available, structural data on apo-kinesin-1 in the absence of tubulin are still missing, leaving the role of nucleotide release in the structural cycle unsettled. Here, we identified mutations in the kinesin nucleotide-binding P-loop motif that interfere with ADP binding. These mutations destabilize the P-loop (T87A mutant) or magnesium binding (T92V), highlighting a dual mechanism for nucleotide release. The structures of these mutants in their apo form are either isomorphous to ADP-kinesin-1 or to tubulin-bound apo-kinesin-1. Remarkably, both structures are also obtained from the nucleotide-depleted wild-type protein. Our results lead to a model in which, when detached from microtubules, apo-kinesin possibly occupies the two conformations we characterized, whereas, upon microtubule binding, ADP-kinesin converts to the tubulin-bound apo-kinesin conformation and releases ADP. This conformation is primed to bind ATP and, therefore, to run through the natural nucleotide cycle of kinesin-1.

-	~~Authors~~:	+	The structural switch of nucleotide-free kinesin.,Cao L, Cantos-Fernandes S, Gigant B Sci Rep. 2017 Feb 14;7:42558. doi: 10.1038/srep42558. PMID:28195215<ref>PMID:28195215</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5lt0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cao, L]]
		+	[[Category: Gigant, B]]
		+	[[Category: Adp dissociation]]
		+	[[Category: Kinesin motor domain]]
		+	[[Category: Motor protein]]
		+	[[Category: Nucleotide-free]]

OCA at 14:46, 19 October 2016

OCA — Wed, 19 Oct 2016 14:46:11 GMT

←Older revision		Revision as of 14:46, 19 October 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5lt0 is ON HOLD	+	The entry 5lt0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5lt0" [edit=sysop:move=sysop]

OCA — Wed, 14 Sep 2016 13:39:35 GMT

Protected "5lt0" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:39, 14 September 2016

OCA: New page: '''Unreleased structure''' The entry 5lt0 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 14 Sep 2016 13:39:34 GMT

New page: '''Unreleased structure''' The entry 5lt0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5lt0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]