5lul - Revision history

OCA at 18:50, 18 October 2023

OCA — Wed, 18 Oct 2023 18:50:19 GMT

←Older revision		Revision as of 18:50, 18 October 2023
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	<StructureSection load='5lul' size='340' side='right'caption='[[5lul]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5lul' size='340' side='right'caption='[[5lul]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lul]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LUL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LUL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lul]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_cellulosilytica Thermobifida cellulosilytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LUL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5lul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lul OCA], [~~http~~://pdbe.org/5lul PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lul RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lul PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lul ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lul OCA], [https://pdbe.org/5lul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lul RCSB], [https://www.ebi.ac.uk/pdbsum/5lul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lul ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PETH2_THECS PETH2_THECS] Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle (Ref.1). Shows esterase activity towards p-nitrophenol-linked aliphatic esters (pNP-aliphatic esters) (Ref.1, PubMed:23592055). Capable of degrading the plastic poly(ethylene terephthalate) (PET), the most abundant polyester plastic in the world (Ref.1, PubMed:23592055). Capable of degrading the bioplastic poly(lactic acid) (PLLA) (PubMed:28671263).<ref>PMID:23592055</ref> <ref>PMID:28671263</ref> [UniProtKB:A0A0K8P6T7]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Gruber, K~~]]	+	[[Category: Thermobifida cellulosilytica]]
-	[[Category: ~~Hromic, A~~]]	+	[[Category: Gruber K]]
-	[[Category: ~~Lyskowski,~~ A]]	+	[[Category: Hromic A]]
-	[[Category: ~~Alpha/beta hydrolase]]~~	+	[[Category: Lyskowski A]]
-	~~[[Category: Cutinase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 10:50, 12 August 2020

OCA — Wed, 12 Aug 2020 10:50:50 GMT

←Older revision		Revision as of 10:50, 12 August 2020
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	==Structure of a triple variant of cutinase 2 from Thermobifida cellulosilytica==		==Structure of a triple variant of cutinase 2 from Thermobifida cellulosilytica==
-	<StructureSection load='5lul' size='340' side='right' caption='[[5lul]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5lul' size='340' side='right'caption='[[5lul]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lul]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LUL OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LUL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lul]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LUL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LUL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lul OCA], [http://pdbe.org/5lul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lul RCSB], [http://www.ebi.ac.uk/pdbsum/5lul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lul ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lul OCA], [http://pdbe.org/5lul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lul RCSB], [http://www.ebi.ac.uk/pdbsum/5lul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lul ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5lul" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lul" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cutinase 3D structures\|Cutinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gruber, K]]		[[Category: Gruber, K]]
	[[Category: Hromic, A]]		[[Category: Hromic, A]]

OCA at 09:15, 9 August 2017

OCA — Wed, 09 Aug 2017 09:15:10 GMT

←Older revision		Revision as of 09:15, 9 August 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5lul is ~~ON HOLD until Paper~~ Publication	+	==Structure of a triple variant of cutinase 2 from Thermobifida cellulosilytica==
		+	<StructureSection load='5lul' size='340' side='right' caption='[[5lul]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lul]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LUL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lul OCA], [http://pdbe.org/5lul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lul RCSB], [http://www.ebi.ac.uk/pdbsum/5lul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lul ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have investigated the structures of two native cutinases from Thermobifida cellulosilytica, namely Thc_Cut1 and Thc_Cut2 as well as of two variants, Thc_Cut2_DM (Thc_Cut2_ Arg29Asn_Ala30Val) and Thc_Cut2_TM (Thc_Cut2_Arg19Ser_Arg29Asn_Ala30Val). The four enzymes showed different activities towards the aliphatic polyester poly(lactic acid) (PLLA). The crystal structures of the four enzymes were successfully solved and in combination with Small Angle X-Ray Scattering (SAXS) the structural features responsible for the selectivity difference were elucidated. Analysis of the crystal structures did not indicate significant conformational differences among the different cutinases. However, the distinctive SAXS scattering data collected from the enzymes in solution indicated a remarkable surface charge difference. The difference in the electrostatic and hydrophobic surface properties could explain potential alternative binding modes of the four cutinases on PLLA explaining their distinct activities. This article is protected by copyright. All rights reserved.

-	~~Authors~~: Hromic, A., ~~Lyskowski~~, A., ~~Gruber~~, K.	+	Small cause, large effect: Structural characterization of cutinases from Thermobifida cellulosilytica.,Ribitsch D, Hromic A, Zitzenbacher S, Zartl B, Gamerith C, Pellis A, Jungbauer A, Lyskowski A, Steinkellner G, Gruber K, Tscheliessnig R, Acero EH, Guebitz GM Biotechnol Bioeng. 2017 Jul 3. doi: 10.1002/bit.26372. PMID:28671263<ref>PMID:28671263</ref>

-	~~Description: Structure of~~ a ~~triple variant~~ of ~~cutinase 2 from Thermobifida cellulosilytica~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Hromic, A]]~~	+	<div class="pdbe-citations 5lul" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Gruber, K]]		[[Category: Gruber, K]]
		+	[[Category: Hromic, A]]
	[[Category: Lyskowski, A]]		[[Category: Lyskowski, A]]
		+	[[Category: Alpha/beta hydrolase]]
		+	[[Category: Cutinase]]
		+	[[Category: Hydrolase]]

OCA at 10:53, 9 March 2017

OCA — Thu, 09 Mar 2017 10:53:18 GMT

←Older revision		Revision as of 10:53, 9 March 2017
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	Description: Structure of a triple variant of cutinase 2 from Thermobifida cellulosilytica		Description: Structure of a triple variant of cutinase 2 from Thermobifida cellulosilytica
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Lyskowski, A]]
	[[Category: Hromic, A]]		[[Category: Hromic, A]]
	[[Category: Gruber, K]]		[[Category: Gruber, K]]
		+	[[Category: Lyskowski, A]]

OCA at 14:43, 3 October 2016

OCA — Mon, 03 Oct 2016 14:43:35 GMT

←Older revision		Revision as of 14:43, 3 October 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5lul is ON HOLD	+	The entry 5lul is ON HOLD until Paper Publication

	Authors: Hromic, A., Lyskowski, A., Gruber, K.		Authors: Hromic, A., Lyskowski, A., Gruber, K.

-	Description: ~~Structural characterization~~ of triple ~~mutant~~ of ~~cutinase2~~ from Thermobifida cellulosilytica	+	Description: Structure of a triple variant of cutinase 2 from Thermobifida cellulosilytica
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Lyskowski, A]]		[[Category: Lyskowski, A]]
	[[Category: Hromic, A]]		[[Category: Hromic, A]]
	[[Category: Gruber, K]]		[[Category: Gruber, K]]

OCA: Protected "5lul" [edit=sysop:move=sysop]

OCA — Wed, 14 Sep 2016 13:43:24 GMT

Protected "5lul" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:43, 14 September 2016

OCA: New page: '''Unreleased structure''' The entry 5lul is ON HOLD Authors: Hromic, A., Lyskowski, A., Gruber, K. Description: Structural characterization of triple mutant of cutinase2 from Thermobi...

OCA — Wed, 14 Sep 2016 13:43:23 GMT

New page: '''Unreleased structure''' The entry 5lul is ON HOLD Authors: Hromic, A., Lyskowski, A., Gruber, K. Description: Structural characterization of triple mutant of cutinase2 from Thermobi...

New page

'''Unreleased structure'''

The entry 5lul is ON HOLD

Authors: Hromic, A., Lyskowski, A., Gruber, K.

Description: Structural characterization of triple mutant of cutinase2 from Thermobifida cellulosilytica
[[Category: Unreleased Structures]]
[[Category: Lyskowski, A]]
[[Category: Hromic, A]]
[[Category: Gruber, K]]