5lxa - Revision history

OCA at 18:55, 18 October 2023

OCA — Wed, 18 Oct 2023 18:55:16 GMT

←Older revision		Revision as of 18:55, 18 October 2023
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	<StructureSection load='5lxa' size='340' side='right'caption='[[5lxa]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='5lxa' size='340' side='right'caption='[[5lxa]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lxa]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LXA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lxa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LXA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ADIPOR2, PAQR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxa OCA], [~~http~~://pdbe.org/5lxa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lxa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lxa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxa ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxa OCA], [https://pdbe.org/5lxa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lxa RCSB], [https://www.ebi.ac.uk/pdbsum/5lxa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~ADR2_HUMAN ADR2_HUMAN]~~] Receptor for ~~globular and full-length adiponectin (APM1)~~, an essential hormone secreted by adipocytes that ~~acts as an antidiabetic. Probably involved in metabolic pathways that regulate~~ lipid metabolism ~~such as fatty acid oxidation~~. ~~Mediates~~ increased ~~AMPK,~~ PPARA ~~ligand~~ activity, fatty acid oxidation and glucose uptake ~~by adiponectin~~. Has ~~some~~ intermediate-affinity ~~receptor activity~~ for ~~both~~ globular and full-length adiponectin.<ref>PMID:12802337</ref>	+	[https://www.uniprot.org/uniprot/PAQR2_HUMAN PAQR2_HUMAN] Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (PubMed:12802337, PubMed:25855295). Required for normal body fat and glucose homeostasis. ADIPOQ-binding activates a signaling cascade that leads to increased PPARA activity, and ultimately to increased fatty acid oxidation and glucose uptake. Has intermediate affinity for globular and full-length adiponectin. Required for normal revascularization after chronic ischemia caused by severing of blood vessels (By similarity).[UniProtKB:Q8BQS5]<ref>PMID:12802337</ref> <ref>PMID:25855295</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Granier, S]]	+	[[Category: Granier S]]
-	[[Category: Hoh, F]]	+	[[Category: Hoh F]]
-	[[Category: Leyrat, C]]	+	[[Category: Leyrat C]]
-	[[Category: Vasiliauskaite-Brooks, I]]	+	[[Category: Vasiliauskaite-Brooks I]]
-	~~[[Category: Ceramidase]]~~	+
-	~~[[Category: Integral membrane protein]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Progestin and adipoq receptor family~~]]	+

OCA at 06:32, 16 October 2019

OCA — Wed, 16 Oct 2019 06:32:19 GMT

←Older revision		Revision as of 06:32, 16 October 2019
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	==CRYSTAL STRUCTURE OF HUMAN ADIPONECTIN RECEPTOR 2 IN COMPLEX WITH A C18 FREE FATTY ACID AT 3.0 ANGSTROM RESOLUTION==		==CRYSTAL STRUCTURE OF HUMAN ADIPONECTIN RECEPTOR 2 IN COMPLEX WITH A C18 FREE FATTY ACID AT 3.0 ANGSTROM RESOLUTION==
-	<StructureSection load='5lxa' size='340' side='right' caption='[[5lxa]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='5lxa' size='340' side='right'caption='[[5lxa]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lxa]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LXA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lxa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LXA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ADIPOR2, PAQR2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxa OCA], [http://pdbe.org/5lxa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lxa RCSB], [http://www.ebi.ac.uk/pdbsum/5lxa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxa OCA], [http://pdbe.org/5lxa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lxa RCSB], [http://www.ebi.ac.uk/pdbsum/5lxa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxa ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 5lxa" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lxa" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Granier, S]]		[[Category: Granier, S]]
	[[Category: Hoh, F]]		[[Category: Hoh, F]]

OCA at 06:56, 5 April 2017

OCA — Wed, 05 Apr 2017 06:56:05 GMT

←Older revision		Revision as of 06:56, 5 April 2017
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ADR2_HUMAN ADR2_HUMAN]] Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.<ref>PMID:12802337</ref>		[[http://www.uniprot.org/uniprot/ADR2_HUMAN ADR2_HUMAN]] Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.<ref>PMID:12802337</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Adiponectin receptors (ADIPORs) are integral membrane proteins that control glucose and lipid metabolism by mediating, at least in part, a cellular ceramidase activity that catalyses the hydrolysis of ceramide to produce sphingosine and a free fatty acid (FFA). The crystal structures of the two receptor subtypes, ADIPOR1 and ADIPOR2, show a similar overall seven-transmembrane-domain architecture with large unoccupied cavities and a zinc binding site within the seven transmembrane domain. However, the molecular mechanisms by which ADIPORs function are not known. Here we describe the crystal structure of ADIPOR2 bound to a FFA molecule and show that ADIPOR2 possesses intrinsic basal ceramidase activity that is enhanced by adiponectin. We also identify a ceramide binding pose and propose a possible mechanism for the hydrolytic activity of ADIPOR2 using computational approaches. In molecular dynamics simulations, the side chains of residues coordinating the zinc rearrange quickly to promote the nucleophilic attack of a zinc-bound hydroxide ion onto the ceramide amide carbonyl. Furthermore, we present a revised ADIPOR1 crystal structure exhibiting a seven-transmembrane-domain architecture that is clearly distinct from that of ADIPOR2. In this structure, no FFA is observed and the ceramide binding pocket and putative zinc catalytic site are exposed to the inner membrane leaflet. ADIPOR1 also possesses intrinsic ceramidase activity, so we suspect that the two distinct structures may represent key steps in the enzymatic activity of ADIPORs. The ceramidase activity is low, however, and further studies will be required to characterize fully the enzymatic parameters and substrate specificity of ADIPORs. These insights into ADIPOR function will enable the structure-based design of potent modulators of these clinically relevant enzymes.
		+
		+	Structural insights into adiponectin receptors suggest ceramidase activity.,Vasiliauskaite-Brooks I, Sounier R, Rochaix P, Bellot G, Fortier M, Hoh F, De Colibus L, Bechara C, Saied EM, Arenz C, Leyrat C, Granier S Nature. 2017 Mar 22. doi: 10.1038/nature21714. PMID:28329765<ref>PMID:28329765</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5lxa" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:23, 22 March 2017

OCA — Wed, 22 Mar 2017 16:23:34 GMT

←Older revision		Revision as of 16:23, 22 March 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5lxa is ~~ON HOLD~~ ~~until Paper Publication~~	+	==CRYSTAL STRUCTURE OF HUMAN ADIPONECTIN RECEPTOR 2 IN COMPLEX WITH A C18 FREE FATTY ACID AT 3.0 ANGSTROM RESOLUTION==
-		+	<StructureSection load='5lxa' size='340' side='right' caption='[[5lxa]], [[Resolution\|resolution]] 3.00Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5lxa]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LXA FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxa OCA], [http://pdbe.org/5lxa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lxa RCSB], [http://www.ebi.ac.uk/pdbsum/5lxa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxa ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ADR2_HUMAN ADR2_HUMAN]] Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.<ref>PMID:12802337</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Granier, S]]
		+	[[Category: Hoh, F]]
		+	[[Category: Leyrat, C]]
		+	[[Category: Vasiliauskaite-Brooks, I]]
		+	[[Category: Ceramidase]]
		+	[[Category: Integral membrane protein]]
		+	[[Category: Membrane protein]]
		+	[[Category: Progestin and adipoq receptor family]]

OCA at 19:18, 5 October 2016

OCA — Wed, 05 Oct 2016 19:18:53 GMT

←Older revision		Revision as of 19:18, 5 October 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5lxa is ON HOLD	+	The entry 5lxa is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5lxa" [edit=sysop:move=sysop]

OCA — Mon, 03 Oct 2016 14:50:16 GMT

Protected "5lxa" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:50, 3 October 2016

OCA: New page: '''Unreleased structure''' The entry 5lxa is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Mon, 03 Oct 2016 14:50:15 GMT

New page: '''Unreleased structure''' The entry 5lxa is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5lxa is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]