5m3k - Revision history

OCA at 18:23, 1 November 2023

OCA — Wed, 01 Nov 2023 18:23:43 GMT

←Older revision		Revision as of 18:23, 1 November 2023
Line 1:		Line 1:

	==A multi-component acyltransferase PhlABC from Pseudomonas protegens==		==A multi-component acyltransferase PhlABC from Pseudomonas protegens==
-	<StructureSection load='5m3k' size='340' side='right' caption='[[5m3k]], [[Resolution\|resolution]] 2.83Å' scene=''>	+	<StructureSection load='5m3k' size='340' side='right'caption='[[5m3k]], [[Resolution\|resolution]] 2.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5m3k]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus~~ fluorescens ~~liquefaciens" flugge 1886~~] and [~~http~~://en.wikipedia.org/wiki/~~Psef5 Psef5~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M3K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5M3K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5m3k]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens] and [https://en.wikipedia.org/wiki/Pseudomonas_protegens_Pf-5 Pseudomonas protegens Pf-5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M3K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M3K FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.83Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCY:S-ACETYL-CYSTEINE'>SCY</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCY:S-ACETYL-CYSTEINE'>SCY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><td class="sblockDat">phlA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 "Bacillus fluorescens liquefaciens" Flugge 1886]), phlB, PFL_5956 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=220664 PSEF5]), phlC, PFL_5955 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=220664 PSEF5])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m3k OCA], [https://pdbe.org/5m3k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m3k RCSB], [https://www.ebi.ac.uk/pdbsum/5m3k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m3k ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5m3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m3k OCA], [~~http~~://pdbe.org/5m3k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5m3k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5m3k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5m3k ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4K420_PSEF5 Q4K420_PSEF5]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ fluorescens ~~liquefaciens flugge 1886~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Psef5~~]]	+	[[Category: Pseudomonas fluorescens]]
-	[[Category: Gruber, K]]	+	[[Category: Pseudomonas protegens Pf-5]]
-	[[Category: Kroutil, W]]	+	[[Category: Gruber K]]
-	[[Category: Pavkov-Keller, T]]	+	[[Category: Kroutil W]]
-	[[Category: Schmidt~~, N G]]~~	+	[[Category: Pavkov-Keller T]]
-	~~[[Category: Acyltransferase]]~~	+	[[Category: Schmidt NG]]
-	~~[[Category: Multi-component]]~~	+
-	~~[[Category: Phlabc]]~~	+
-	~~[[Category: Pseudomonas protegen]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:06, 31 October 2018

OCA — Wed, 31 Oct 2018 07:06:17 GMT

←Older revision		Revision as of 07:06, 31 October 2018
Line 3:		Line 3:
	<StructureSection load='5m3k' size='340' side='right' caption='[[5m3k]], [[Resolution\|resolution]] 2.83Å' scene=''>		<StructureSection load='5m3k' size='340' side='right' caption='[[5m3k]], [[Resolution\|resolution]] 2.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5m3k]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M3K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5m3k]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus fluorescens liquefaciens" flugge 1886] and [http://en.wikipedia.org/wiki/Psef5 Psef5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M3K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCY:S-ACETYL-CYSTEINE'>SCY</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCY:S-ACETYL-CYSTEINE'>SCY</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phlA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 "Bacillus fluorescens liquefaciens" Flugge 1886]), phlB, PFL_5956 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=220664 PSEF5]), phlC, PFL_5955 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=220664 PSEF5])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m3k OCA], [http://pdbe.org/5m3k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m3k RCSB], [http://www.ebi.ac.uk/pdbsum/5m3k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m3k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m3k OCA], [http://pdbe.org/5m3k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m3k RCSB], [http://www.ebi.ac.uk/pdbsum/5m3k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m3k ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	C-C bond forming reactions are key transformations to set up the carbon framework of organic molecules. In this context, the Friedel-Crafts acylation is commonly used for the synthesis of aryl ketones, which are common motifs in many fine chemicals and natural products. A bacterial multi-component acyltransferase from Pseudomonas protegens (PpATase) catalyzes such a Friedel-Crafts C-acylation of phenolic substrates in aqueous solution reaching up to >99% conversion without the need of CoA-activated reagents. We determined x-ray crystal structures of the native and ligand-bound complex. This multimeric enzyme consists of three subunits - PhlA, PhlB and PhlC which are arranged in a Phl(A2C2)2B4 composition. The structure of a reaction intermediate obtained from crystals soaked with the natural substrate monoacetylphloroglucinol together with site-directed mutagenesis studies revealed that only residues from the PhlC subunits are involved in the acyl transfer reaction, with Cys88 very likely playing a significant role during catalysis. These structural and mechanistic insights form the basis of further enzyme engineering efforts towards enhancing the substrate scope of this enzyme.
		+
		+	Structure and catalytic mechanism of a bacterial Friedel-Crafts acylase.,Pavkov-Keller T, Schmidt NG, Zadlo-Dobrowolska A, Kroutil W, Gruber K Chembiochem. 2018 Oct 14. doi: 10.1002/cbic.201800462. PMID:30318713<ref>PMID:30318713</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5m3k" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus fluorescens liquefaciens flugge 1886]]
		+	[[Category: Psef5]]
	[[Category: Gruber, K]]		[[Category: Gruber, K]]
	[[Category: Kroutil, W]]		[[Category: Kroutil, W]]

OCA at 06:04, 20 December 2017

OCA — Wed, 20 Dec 2017 06:04:22 GMT

←Older revision		Revision as of 06:04, 20 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5m3k is ~~ON HOLD until Paper Publication~~	+	==A multi-component acyltransferase PhlABC from Pseudomonas protegens==
-		+	<StructureSection load='5m3k' size='340' side='right' caption='[[5m3k]], [[Resolution\|resolution]] 2.83Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5m3k]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M3K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M3K FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCY:S-ACETYL-CYSTEINE'>SCY</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m3k OCA], [http://pdbe.org/5m3k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m3k RCSB], [http://www.ebi.ac.uk/pdbsum/5m3k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m3k ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gruber, K]]
		+	[[Category: Kroutil, W]]
		+	[[Category: Pavkov-Keller, T]]
		+	[[Category: Schmidt, N G]]
		+	[[Category: Acyltransferase]]
		+	[[Category: Multi-component]]
		+	[[Category: Phlabc]]
		+	[[Category: Pseudomonas protegen]]
		+	[[Category: Transferase]]

OCA at 05:29, 25 October 2017

OCA — Wed, 25 Oct 2017 05:29:06 GMT

←Older revision		Revision as of 05:29, 25 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5m3k is ON HOLD	+	The entry 5m3k is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5m3k" [edit=sysop:move=sysop]

OCA — Wed, 26 Oct 2016 16:21:11 GMT

Protected "5m3k" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:21, 26 October 2016

OCA: New page: '''Unreleased structure''' The entry 5m3k is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Oct 2016 16:21:10 GMT

New page: '''Unreleased structure''' The entry 5m3k is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5m3k is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]