5m52 - Revision history

OCA at 18:26, 1 November 2023

OCA — Wed, 01 Nov 2023 18:26:12 GMT

←Older revision		Revision as of 18:26, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='5m52' size='340' side='right'caption='[[5m52]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='5m52' size='340' side='right'caption='[[5m52]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5m52]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M52 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5M52 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5m52]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M52 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>BRR2, RSS1, SNU246, YER172C, SYGP-ORF66 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=4932 ATCC 18824]), PRP8, DBF3, DNA39, RNA8, SLT21, USA2, YHR165C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.4Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>~~[~~http://en.wikipedia.org/wiki/RNA_helicase RNA helicase~~]~~, with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~3.6.4~~.13 3.6.4.13] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m52 OCA], [https://pdbe.org/5m52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m52 RCSB], [https://www.ebi.ac.uk/pdbsum/5m52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m52 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5m52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m52 OCA], [~~http~~://pdbe.org/5m52 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5m52 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5m52 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5m52 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BRR2_YEAST BRR2_YEAST]] RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.<ref>PMID:19098916</ref> <ref>PMID:23124065</ref> <ref>PMID:19716790</ref> <ref>PMID:19525970</ref> [[http://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>	+	[https://www.uniprot.org/uniprot/BRR2_YEAST BRR2_YEAST] RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.<ref>PMID:19098916</ref> <ref>PMID:23124065</ref> <ref>PMID:19716790</ref> <ref>PMID:19525970</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~RNA helicase~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Absmeier, E]]	+	[[Category: Absmeier E]]
-	[[Category: Becke, C]]	+	[[Category: Becke C]]
-	[[Category: Santos~~, K F~~]]	+	[[Category: Santos KF]]
-	[[Category: Wahl~~, M C~~]]	+	[[Category: Wahl MC]]
-	[[Category: Wollenhaupt, J]]	+	[[Category: Wollenhaupt J]]
-	~~[[Category: Helicase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein complex~~]]	+

OCA at 11:01, 12 August 2020

OCA — Wed, 12 Aug 2020 11:01:45 GMT

←Older revision		Revision as of 11:01, 12 August 2020
Line 1:		Line 1:

	==Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain==		==Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain==
-	<StructureSection load='5m52' size='340' side='right' caption='[[5m52]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<StructureSection load='5m52' size='340' side='right'caption='[[5m52]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5m52]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M52 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5M52 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5m52]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M52 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5M52 FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BRR2, RSS1, SNU246, YER172C, SYGP-ORF66 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PRP8, DBF3, DNA39, RNA8, SLT21, USA2, YHR165C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5m52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m52 OCA], [http://pdbe.org/5m52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m52 RCSB], [http://www.ebi.ac.uk/pdbsum/5m52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m52 ProSAT]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5m52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m52 OCA], [http://pdbe.org/5m52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m52 RCSB], [http://www.ebi.ac.uk/pdbsum/5m52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m52 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5m52" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5m52" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Helicase 3D structures\|Helicase 3D structures]]
		+	*[[Pre-mRNA splicing factors 3D structures\|Pre-mRNA splicing factors 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: RNA helicase]]		[[Category: RNA helicase]]
	[[Category: Absmeier, E]]		[[Category: Absmeier, E]]

OCA at 19:38, 9 December 2016

OCA — Fri, 09 Dec 2016 19:38:48 GMT

←Older revision		Revision as of 19:38, 9 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5m52 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain==
		+	<StructureSection load='5m52' size='340' side='right' caption='[[5m52]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5m52]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M52 FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m52 OCA], [http://pdbe.org/5m52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m52 RCSB], [http://www.ebi.ac.uk/pdbsum/5m52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m52 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BRR2_YEAST BRR2_YEAST]] RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.<ref>PMID:19098916</ref> <ref>PMID:23124065</ref> <ref>PMID:19716790</ref> <ref>PMID:19525970</ref> [[http://www.uniprot.org/uniprot/PRP8_YEAST PRP8_YEAST]] Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region. Also has a role in cell cycle.<ref>PMID:2835658</ref> <ref>PMID:9150140</ref> <ref>PMID:12773561</ref> <ref>PMID:18779563</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RNA helicase Brr2 is implicated in multiple phases of pre-mRNA splicing and thus requires tight regulation. Brr2 can be auto-inhibited via a large N-terminal region folding back onto its helicase core and auto-activated by a catalytically inactive C-terminal helicase cassette. Furthermore, it can be regulated in trans by the Jab1 domain of the Prp8 protein, which can inhibit Brr2 by intermittently inserting a C-terminal tail in the enzyme's RNA-binding tunnel or activate the helicase after removal of this tail. Presently it is unclear, whether these regulatory mechanisms functionally interact and to which extent they are evolutionarily conserved. Here, we report crystal structures of Saccharomyces cerevisiae and Chaetomium thermophilum Brr2-Jab1 complexes, demonstrating that Jab1-based inhibition of Brr2 presumably takes effect in all eukaryotes but is implemented via organism-specific molecular contacts. Moreover, the structures show that Brr2 auto-inhibition can act in concert with Jab1-mediated inhibition, and suggest that the N-terminal region influences how the Jab1 C-terminal tail interacts at the RNA-binding tunnel. Systematic RNA binding and unwinding studies revealed that the N-terminal region and the Jab1 C-terminal tail specifically interfere with accommodation of double-stranded and single-stranded regions of an RNA substrate, respectively, mutually reinforcing each other. Additionally, such analyses show that regulation based on the N-terminal region requires the presence of the inactive C-terminal helicase cassette. Together, our results outline an intricate system of regulatory mechanisms, which control Brr2 activities during snRNP assembly and splicing.

-	~~Authors~~:	+	Interplay of cis- and trans-regulatory mechanisms in the spliceosomal RNA helicase Brr2.,Absmeier E, Becke C, Wollenhaupt J, Santos KF, Wahl MC Cell Cycle. 2016 Nov 23:0. PMID:27880071<ref>PMID:27880071</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5m52" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: RNA helicase]]
		+	[[Category: Absmeier, E]]
		+	[[Category: Becke, C]]
		+	[[Category: Santos, K F]]
		+	[[Category: Wahl, M C]]
		+	[[Category: Wollenhaupt, J]]
		+	[[Category: Helicase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protein complex]]

OCA: Protected "5m52" [edit=sysop:move=sysop]

OCA — Wed, 26 Oct 2016 16:25:38 GMT

Protected "5m52" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:25, 26 October 2016

OCA: New page: '''Unreleased structure''' The entry 5m52 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Oct 2016 16:25:37 GMT

New page: '''Unreleased structure''' The entry 5m52 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5m52 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]