5m9x - Revision history

OCA at 18:34, 1 November 2023

OCA — Wed, 01 Nov 2023 18:34:15 GMT

←Older revision		Revision as of 18:34, 1 November 2023
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	<StructureSection load='5m9x' size='340' side='right'caption='[[5m9x]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='5m9x' size='340' side='right'caption='[[5m9x]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5m9x]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_1.2190 As 1.2190~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M9X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5M9X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5m9x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium_adolescentis Bifidobacterium adolescentis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M9X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7KP:(2~{R},3~{S},4~{S},5~{R},6~{R})-2-(hydroxymethyl)-6-[3-[(~{E})-2-(4-hydroxyphenyl)ethenyl]-5-oxidanyl-phenoxy]oxane-3,4,5-triol'>7KP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.349Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5c8b\|5c8b]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7KP:(2~{R},3~{S},4~{S},5~{R},6~{R})-2-(hydroxymethyl)-6-[3-[(~{E})-2-(4-hydroxyphenyl)ethenyl]-5-oxidanyl-phenoxy]oxane-3,4+,5-triol'>7KP</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sucP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1680 AS 1.2190])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m9x OCA], [https://pdbe.org/5m9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m9x RCSB], [https://www.ebi.ac.uk/pdbsum/5m9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m9x ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.7 2.4.1.7] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5m9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m9x OCA], [~~http~~://pdbe.org/5m9x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5m9x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5m9x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5m9x ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SUCP_BIFAA SUCP_BIFAA] Catalyzes the reversible phosphorolysis of sucrose into alpha-D-glucose 1-phosphate (Glc1P) and D-fructose (PubMed:14740189, PubMed:20691225). Is involved in sucrose degradation. Also displays transglucosylation activity in vitro, by transferring the glucosyl moiety of Glc1P to a broad range of monomeric sugars, such as D- and L-arabinose, D- and L-arabitol, and xylitol (PubMed:14740189).<ref>PMID:14740189</ref> <ref>PMID:20691225</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~As 1 2190~~]]	+	[[Category: Bifidobacterium adolescentis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Transferase]]~~	+	[[Category: Grimm C]]
-	[[Category: Grimm, C]]	+	[[Category: Kraus M]]
-	[[Category: Kraus, M]]	+
-	~~[[Category: Enzyme design]]~~	+
-	~~[[Category: Resveratrol]]~~	+
-	~~[[Category: Sucrose phosphorylase~~]]	+

OCA at 06:35, 16 October 2019

OCA — Wed, 16 Oct 2019 06:35:21 GMT

←Older revision		Revision as of 06:35, 16 October 2019
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	==Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to glycosylated resveratrol==		==Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to glycosylated resveratrol==
-	<StructureSection load='5m9x' size='340' side='right' caption='[[5m9x]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='5m9x' size='340' side='right'caption='[[5m9x]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5m9x]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M9X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5m9x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_1.2190 As 1.2190]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M9X FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7KP:(2~{R},3~{S},4~{S},5~{R},6~{R})-2-(hydroxymethyl)-6-[3-[(~{E})-2-(4-hydroxyphenyl)ethenyl]-5-oxidanyl-phenoxy]oxane-3,4,5-triol'>7KP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7KP:(2~{R},3~{S},4~{S},5~{R},6~{R})-2-(hydroxymethyl)-6-[3-[(~{E})-2-(4-hydroxyphenyl)ethenyl]-5-oxidanyl-phenoxy]oxane-3,4,5-triol'>7KP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5c8b\|5c8b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5c8b\|5c8b]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sucP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1680 AS 1.2190])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.7 2.4.1.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.7 2.4.1.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m9x OCA], [http://pdbe.org/5m9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m9x RCSB], [http://www.ebi.ac.uk/pdbsum/5m9x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m9x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m9x OCA], [http://pdbe.org/5m9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m9x RCSB], [http://www.ebi.ac.uk/pdbsum/5m9x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m9x ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 1 2190]]
		+	[[Category: Large Structures]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Grimm, C]]		[[Category: Grimm, C]]

OCA at 06:05, 20 December 2017

OCA — Wed, 20 Dec 2017 06:05:36 GMT

←Older revision		Revision as of 06:05, 20 December 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5m9x is ~~ON HOLD~~	+	==Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to glycosylated resveratrol==
		+	<StructureSection load='5m9x' size='340' side='right' caption='[[5m9x]], [[Resolution\|resolution]] 2.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5m9x]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M9X FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7KP:(2~{R},3~{S},4~{S},5~{R},6~{R})-2-(hydroxymethyl)-6-[3-[(~{E})-2-(4-hydroxyphenyl)ethenyl]-5-oxidanyl-phenoxy]oxane-3,4,5-triol'>7KP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5c8b\|5c8b]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.7 2.4.1.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m9x OCA], [http://pdbe.org/5m9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m9x RCSB], [http://www.ebi.ac.uk/pdbsum/5m9x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m9x ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Here we present a point mutation-triggered domain shift which switches the acceptor preference of a sucrose phosphorylase from phosphate to a variety of large polyphenolic compounds including resveratrol and quercetin, enabling their efficient glucosylation. The variant possesses a high affinity for aromatic substrates due to newly introduced pi-pi- and hydrophobic interactions in the altered active site. The domain shift brings about a substantially enlarged and multifunctional active site for polyphenol glucosylation and rare disaccharide production. The crystal structure of the variant with its product resveratrol-3-alpha-d-glucoside allows the prediction of the substrate scope and regioselectivity of the aromatic compounds' glucosylation sites.

-	~~Authors: Grimm, C~~., Kraus, M.	+	Switching enzyme specificity from phosphate to resveratrol glucosylation.,Kraus M, Grimm C, Seibel J Chem Commun (Camb). 2017 Nov 9;53(90):12181-12184. doi: 10.1039/c7cc05993k. PMID:29057405<ref>PMID:29057405</ref>

-	~~Description: Structure~~ of ~~sucrose phosphorylase from Bifidobacterium adolescentis bound to glycosylated resveratrol~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Kraus, M~~]]	+	<div class="pdbe-citations 5m9x" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Transferase]]
	[[Category: Grimm, C]]		[[Category: Grimm, C]]
		+	[[Category: Kraus, M]]
		+	[[Category: Enzyme design]]
		+	[[Category: Resveratrol]]
		+	[[Category: Sucrose phosphorylase]]

OCA: Protected "5m9x" [edit=sysop:move=sysop]

OCA — Fri, 09 Dec 2016 16:32:35 GMT

Protected "5m9x" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:32, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5m9x is ON HOLD Authors: Grimm, C., Kraus, M. Description: Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to glycosylat...

OCA — Fri, 09 Dec 2016 16:32:33 GMT

New page: '''Unreleased structure''' The entry 5m9x is ON HOLD Authors: Grimm, C., Kraus, M. Description: Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to glycosylat...

New page

'''Unreleased structure'''

The entry 5m9x is ON HOLD

Authors: Grimm, C., Kraus, M.

Description: Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to glycosylated resveratrol
[[Category: Unreleased Structures]]
[[Category: Kraus, M]]
[[Category: Grimm, C]]