5mbg - Revision history

OCA at 18:37, 1 November 2023

OCA — Wed, 01 Nov 2023 18:37:09 GMT

←Older revision		Revision as of 18:37, 1 November 2023
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	<StructureSection load='5mbg' size='340' side='right'caption='[[5mbg]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5mbg' size='340' side='right'caption='[[5mbg]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mbg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Beggiatoa_sp.~~_ps~~ Beggiatoa sp. ps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MBG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5MBG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Beggiatoa_sp._PS Beggiatoa sp. PS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MBG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~BGP_1043 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=422289 Beggiatoa sp. PS])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5mbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mbg OCA], [~~http~~://pdbe.org/5mbg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mbg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mbg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mbg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mbg OCA], [https://pdbe.org/5mbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mbg RCSB], [https://www.ebi.ac.uk/pdbsum/5mbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mbg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A7BT71_9GAMM A7BT71_9GAMM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beggiatoa sp. ps]]	+	[[Category: Beggiatoa sp. PS]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Frey, D]]	+	[[Category: Frey D]]
-	[[Category: Hartmann, E]]	+	[[Category: Hartmann E]]
-	[[Category: Lindner, R]]	+	[[Category: Lindner R]]
-	[[Category: Meinhart, A]]	+	[[Category: Meinhart A]]
-	[[Category: Reinstein, J]]	+	[[Category: Reinstein J]]
-	[[Category: Schlichting, I]]	+	[[Category: Schlichting I]]
-	[[Category: Tarnawski, M]]	+	[[Category: Tarnawski M]]
-	[[Category: Winkler, A]]	+	[[Category: Winkler A]]
-	~~[[Category: Adenylyl cyclase]]~~	+
-	~~[[Category: Bluf]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Optogenetic]]~~	+
-	~~[[Category: Photoreceptor~~]]	+

OCA at 07:15, 19 August 2020

OCA — Wed, 19 Aug 2020 07:15:05 GMT

←Older revision		Revision as of 07:15, 19 August 2020
Line 1:		Line 1:

	==Structure of a bacterial light-regulated adenylyl cyclase==		==Structure of a bacterial light-regulated adenylyl cyclase==
-	<StructureSection load='5mbg' size='340' side='right' caption='[[5mbg]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5mbg' size='340' side='right'caption='[[5mbg]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mbg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MBG OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MBG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Beggiatoa_sp._ps Beggiatoa sp. ps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MBG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MBG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mbg OCA], [http://pdbe.org/5mbg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mbg RCSB], [http://www.ebi.ac.uk/pdbsum/5mbg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mbg ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BGP_1043 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=422289 Beggiatoa sp. PS])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mbg OCA], [http://pdbe.org/5mbg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mbg RCSB], [http://www.ebi.ac.uk/pdbsum/5mbg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mbg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5mbg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5mbg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[3D Adenylyl cyclase 3D structures\|3D Adenylyl cyclase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Beggiatoa sp. ps]]
		+	[[Category: Large Structures]]
	[[Category: Frey, D]]		[[Category: Frey, D]]
	[[Category: Hartmann, E]]		[[Category: Hartmann, E]]

OCA at 13:18, 5 April 2017

OCA — Wed, 05 Apr 2017 13:18:13 GMT

←Older revision		Revision as of 13:18, 5 April 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mbg is ~~ON HOLD~~	+	==Structure of a bacterial light-regulated adenylyl cyclase==
		+	<StructureSection load='5mbg' size='340' side='right' caption='[[5mbg]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mbg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MBG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mbg OCA], [http://pdbe.org/5mbg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mbg RCSB], [http://www.ebi.ac.uk/pdbsum/5mbg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mbg ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Light-regulated enzymes enable organisms to quickly respond to changing light conditions. We characterize a photoactivatable adenylyl cyclase from Beggiatoa sp. (bPAC) that translates a blue light signal into production of the second messenger cyclic AMP. bPAC contains a BLUF photoreceptor domain that senses blue light using a flavin chromophore, linked to an adenylyl cyclase (AC) domain. We present a dark state crystal structure of bPAC that closely resembles the recently published structure of the homologous OaPAC from Oscillatoria acuminata. To elucidate the structural mechanism of light-dependent AC activation by the BLUF domain, we determined crystal structures of illuminated bPAC and of a pseudo-lit state variant. We use hydrogen-deuterium exchange measurements of secondary structure dynamics and hypothesis-driven point mutations to trace the activation pathway from the chromophore in the BLUF domain to the active site of the cyclase. The structural changes are relayed from the residues interacting with the excited chromophore through a conserved kink of the BLUF beta-sheet to a tongue-like extrusion of the AC domain that regulates active site opening and repositions catalytic residues. Our findings not only show the specific molecular pathway of photoactivation in BLUF-regulated ACs, but they also have implications for the general understanding of signaling in BLUF domains and of the activation of adenylyl cyclases.

-	~~Authors:~~ Lindner, R., Hartmann, E., Tarnawski, M., Winkler, A., Frey, D., Reinstein, J., Meinhart, A., Schlichting, I.	+	Photoactivation mechanism of a bacterial light-regulated adenylyl cyclase.,Lindner R, Hartmann E, Tarnawski M, Winkler A, Frey D, Reinstein J, Meinhart A, Schlichting I J Mol Biol. 2017 Mar 20. pii: S0022-2836(17)30124-9. doi:, 10.1016/j.jmb.2017.03.020. PMID:28336405<ref>PMID:28336405</ref>

-	~~Description: Structure of~~ a ~~bacterial light-regulated adenylyl cyclase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Lindner, R]]~~	+	<div class="pdbe-citations 5mbg" style="background-color:#fffaf0;"></div>
-	~~[[Category: Tarnawski, M]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Frey, D]]		[[Category: Frey, D]]
		+	[[Category: Hartmann, E]]
		+	[[Category: Lindner, R]]
	[[Category: Meinhart, A]]		[[Category: Meinhart, A]]
-	[[Category: Schlichting, I]]
	[[Category: Reinstein, J]]		[[Category: Reinstein, J]]
		+	[[Category: Schlichting, I]]
		+	[[Category: Tarnawski, M]]
	[[Category: Winkler, A]]		[[Category: Winkler, A]]
-	[[Category: ~~Hartmann, E~~]]	+	[[Category: Adenylyl cyclase]]
		+	[[Category: Bluf]]
		+	[[Category: Lyase]]
		+	[[Category: Optogenetic]]
		+	[[Category: Photoreceptor]]

OCA at 11:11, 9 March 2017

OCA — Thu, 09 Mar 2017 11:11:07 GMT

←Older revision		Revision as of 11:11, 9 March 2017
Line 7:		Line 7:
	Description: Structure of a bacterial light-regulated adenylyl cyclase		Description: Structure of a bacterial light-regulated adenylyl cyclase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Frey, D]]
-	[[Category: Reinstein, J]]
-	[[Category: Winkler, A]]
	[[Category: Lindner, R]]		[[Category: Lindner, R]]
	[[Category: Tarnawski, M]]		[[Category: Tarnawski, M]]
		+	[[Category: Frey, D]]
	[[Category: Meinhart, A]]		[[Category: Meinhart, A]]
	[[Category: Schlichting, I]]		[[Category: Schlichting, I]]
		+	[[Category: Reinstein, J]]
		+	[[Category: Winkler, A]]
	[[Category: Hartmann, E]]		[[Category: Hartmann, E]]

OCA: Protected "5mbg" [edit=sysop:move=sysop]

OCA — Fri, 09 Dec 2016 16:36:12 GMT

Protected "5mbg" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:36, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5mbg is ON HOLD Authors: Lindner, R., Hartmann, E., Tarnawski, M., Winkler, A., Frey, D., Reinstein, J., Meinhart, A., Schlichting, I. Description:...

OCA — Fri, 09 Dec 2016 16:36:10 GMT

New page: '''Unreleased structure''' The entry 5mbg is ON HOLD Authors: Lindner, R., Hartmann, E., Tarnawski, M., Winkler, A., Frey, D., Reinstein, J., Meinhart, A., Schlichting, I. Description:...

New page

'''Unreleased structure'''

The entry 5mbg is ON HOLD

Authors: Lindner, R., Hartmann, E., Tarnawski, M., Winkler, A., Frey, D., Reinstein, J., Meinhart, A., Schlichting, I.

Description: Structure of a bacterial light-regulated adenylyl cyclase
[[Category: Unreleased Structures]]
[[Category: Frey, D]]
[[Category: Reinstein, J]]
[[Category: Winkler, A]]
[[Category: Lindner, R]]
[[Category: Tarnawski, M]]
[[Category: Meinhart, A]]
[[Category: Schlichting, I]]
[[Category: Hartmann, E]]