5mhr - Revision history

OCA at 18:43, 1 November 2023

OCA — Wed, 01 Nov 2023 18:43:56 GMT

←Older revision		Revision as of 18:43, 1 November 2023
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	<StructureSection load='5mhr' size='340' side='right'caption='[[5mhr]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='5mhr' size='340' side='right'caption='[[5mhr]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mhr]] is a 18 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [~~http~~://en.wikipedia.org/wiki/Reovirus ~~Reovirus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MHR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5MHR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mhr]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Reovirus_sp. Reovirus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MHR FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~S1 (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10891 Reovirus])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5mhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mhr OCA], [~~http~~://pdbe.org/5mhr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mhr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mhr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mhr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mhr OCA], [https://pdbe.org/5mhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mhr RCSB], [https://www.ebi.ac.uk/pdbsum/5mhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mhr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q86335_9REOV Q86335_9REOV]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5mhr" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5mhr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
		+	*[[Virus coat proteins 3D structures\|Virus coat proteins 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Reovirus]]	+	[[Category: Reovirus sp]]
-	[[Category: Dietrich~~, M H~~]]	+	[[Category: Dietrich MH]]
-	[[Category: Stehle, T]]	+	[[Category: Stehle T]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Neutralization]]~~	+
-	~~[[Category: Reovirus sigma1]]~~	+
-	~~[[Category: Viral protein]]~~	+
-	~~[[Category: Virus antibody complex~~]]	+

OCA at 07:20, 19 August 2020

OCA — Wed, 19 Aug 2020 07:20:04 GMT

←Older revision		Revision as of 07:20, 19 August 2020
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	==T3D reovirus sigma1 complexed with 9BG5 Fab fragments==		==T3D reovirus sigma1 complexed with 9BG5 Fab fragments==
-	<StructureSection load='5mhr' size='340' side='right' caption='[[5mhr]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='5mhr' size='340' side='right'caption='[[5mhr]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mhr]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MHR OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MHR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mhr]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Reovirus Reovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MHR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MHR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mhr OCA], [http://pdbe.org/5mhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mhr RCSB], [http://www.ebi.ac.uk/pdbsum/5mhr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mhr ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10891 Reovirus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mhr OCA], [http://pdbe.org/5mhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mhr RCSB], [http://www.ebi.ac.uk/pdbsum/5mhr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mhr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
		+	[[Category: Reovirus]]
	[[Category: Dietrich, M H]]		[[Category: Dietrich, M H]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]

OCA at 17:42, 10 March 2017

OCA — Fri, 10 Mar 2017 17:42:30 GMT

←Older revision		Revision as of 17:42, 10 March 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5mhr is ~~ON HOLD~~	+	==T3D reovirus sigma1 complexed with 9BG5 Fab fragments==
		+	<StructureSection load='5mhr' size='340' side='right' caption='[[5mhr]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mhr]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MHR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MHR FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mhr OCA], [http://pdbe.org/5mhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mhr RCSB], [http://www.ebi.ac.uk/pdbsum/5mhr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mhr ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Reovirus attachment protein sigma1 engages glycan receptors and junctional adhesion molecule-A (JAM-A) and is thought to undergo a conformational change during the proteolytic disassembly of virions to infectious subvirion particles (ISVPs) that accompanies cell entry. The sigma1 protein is also the primary target of neutralizing antibodies. Here we present a structural and functional characterization of two neutralizing antibodies that target sigma1 of serotypes 1 (T1) and 3 (T3) reoviruses. Crystal structures reveal that each antibody engages its cognate sigma1 protein within the head domain via epitopes distinct from the JAM-A-binding site. Surface-plasmon resonance and cell-binding assays indicate that both antibodies likely interfere with JAM-A engagement by steric hindrance. To define the interplay between carbohydrate receptor and antibody binding, we conducted hemagglutination inhibition assays using virions and ISVPs. The glycan-binding site of T1 sigma1 is located in the head domain and is partly occluded by the bound Fab in the crystal structure. The T1-specific antibody inhibited hemagglutination by virions and ISVPs, probably via direct interference with glycan engagement. In contrast to T1 sigma1, the carbohydrate-binding site of T3 sigma1 is located in the tail domain, distal to the antibody epitope. The T3-specific antibody inhibited hemagglutination by T3 virions but not ISVPs, indicating that the antibody- and glycan-binding sites in sigma1 are in closer spatial proximity on virions than on ISVPs. Our results provide direct evidence for a structural rearrangement of sigma1 during virion-to-ISVP conversion and contribute new information about mechanisms of antibody-mediated neutralization of reovirus. IMPORTANCE: Virus attachment proteins mediate binding to host cell receptors, serve critical functions in cell and tissue tropism, and are often targeted by the neutralizing antibody response. The structural investigation of antibody-antigen complexes can provide valuable information for understanding the molecular basis of virus neutralization. Studies with enveloped viruses such as HIV and influenza virus have helped to define sites of vulnerability and guide vaccination strategies. By comparison, less is known about antibody binding to nonenveloped viruses. Here, we structurally investigated two neutralizing antibodies that bind the attachment protein sigma1 of reovirus. We have furthermore characterized the neutralization efficiency, the binding affinity for sigma1, and the effect of the antibodies on reovirus receptor engagement. Our analysis defines reovirus interactions with two neutralizing antibodies, allows us to propose a mechanism by which they block virus infection, and provides evidence for a conformational change in the sigma1 protein during viral cell entry.

-	~~Authors~~:	+	Structural Insights into Reovirus sigma1 Interactions with Two Neutralizing Antibodies.,Dietrich MH, Ogden KM, Katen SP, Reiss K, Sutherland DM, Carnahan RH, Goff M, Cooper T, Dermody TS, Stehle T J Virol. 2016 Dec 7. pii: JVI.01621-16. PMID:27928010<ref>PMID:27928010</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5mhr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Mus musculus]]
		+	[[Category: Dietrich, M H]]
		+	[[Category: Stehle, T]]
		+	[[Category: Immune system]]
		+	[[Category: Neutralization]]
		+	[[Category: Reovirus sigma1]]
		+	[[Category: Viral protein]]
		+	[[Category: Virus antibody complex]]

OCA: Protected "5mhr" [edit=sysop:move=sysop]

OCA — Fri, 09 Dec 2016 16:52:53 GMT

Protected "5mhr" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:52, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5mhr is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Fri, 09 Dec 2016 16:52:52 GMT

New page: '''Unreleased structure''' The entry 5mhr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5mhr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]