5mk9 - Revision history

OCA at 18:47, 1 November 2023

OCA — Wed, 01 Nov 2023 18:47:56 GMT

←Older revision		Revision as of 18:47, 1 November 2023
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	==Maltodextrin binding protein MalE1 from L. casei BL23 bound to beta-cyclodextrin==		==Maltodextrin binding protein MalE1 from L. casei BL23 bound to beta-cyclodextrin==
-	<StructureSection load='5mk9' size='340' side='right' caption='[[5mk9]], [[Resolution\|resolution]] 0.92Å' scene=''>	+	<StructureSection load='5mk9' size='340' side='right'caption='[[5mk9]], [[Resolution\|resolution]] 0.92Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mk9]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_a"_von_freudenreich_1890 "bacillus a" von freudenreich 1890~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MK9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MK9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mk9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lacticaseibacillus_casei Lacticaseibacillus casei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MK9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=BCD:BETA~~-~~CYCLODEXTRIN'>BCD</scene>~~, ~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.919Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5m28\|5m28]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900012:beta-cyclodextrin'>PRD_900012</scene></td></tr>
-	<~~tr id~~='~~gene~~'><~~td class="sblockLbl"~~><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">malE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1582 "Bacillus a" von Freudenreich 1890])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mk9 OCA], [https://pdbe.org/5mk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mk9 RCSB], [https://www.ebi.ac.uk/pdbsum/5mk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mk9 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mk9 OCA], [~~http~~://pdbe.org/5mk9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mk9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mk9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mk9 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B0L7B0_LACCA B0L7B0_LACCA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus a von freudenreich 1890~~]]	+	[[Category: Lacticaseibacillus casei]]
-	[[Category: Beck, S]]	+	[[Category: Large Structures]]
-	[[Category: Bommer, M]]	+	[[Category: Beck S]]
-	[[Category: Deutscher, J]]	+	[[Category: Bommer M]]
-	[[Category: Dobbek, H]]	+	[[Category: Deutscher J]]
-	[[Category: Hobe, C]]	+	[[Category: Dobbek H]]
-	[[Category: Homburg, C]]	+	[[Category: Hobe C]]
-	[[Category: Licht, A]]	+	[[Category: Homburg C]]
-	[[Category: Schneider, E]]	+	[[Category: Licht A]]
-	[[Category: Wuttge, S]]	+	[[Category: Schneider E]]
-	~~[[Category: Carbohydrate binding protein]]~~	+	[[Category: Wuttge S]]
-	~~[[Category: Lactobacillus casei]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 06:31, 16 November 2017

OCA — Thu, 16 Nov 2017 06:31:16 GMT

←Older revision		Revision as of 06:31, 16 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5mk9 is ~~ON HOLD until Paper~~ Publication	+	==Maltodextrin binding protein MalE1 from L. casei BL23 bound to beta-cyclodextrin==
		+	<StructureSection load='5mk9' size='340' side='right' caption='[[5mk9]], [[Resolution\|resolution]] 0.92Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mk9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_a"_von_freudenreich_1890 "bacillus a" von freudenreich 1890]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MK9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCD:BETA-CYCLODEXTRIN'>BCD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5m28\|5m28]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">malE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1582 "Bacillus a" von Freudenreich 1890])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mk9 OCA], [http://pdbe.org/5mk9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mk9 RCSB], [http://www.ebi.ac.uk/pdbsum/5mk9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mk9 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Catabolite repression is a mechanism that enables bacteria to control carbon utilization. As part of this global regulatory network, components of the phosphoenolpyruvate:carbohydrate phosphotransferase system inhibit the uptake of less favorable sugars when a preferred carbon source such as glucose is available. This process is termed inducer exclusion. In bacteria belonging to the phylum Firmicutes, HPr, phosphorylated at serine 46 (P-Ser46-HPr) is the key player but its mode of action is elusive. To address this question at the level of purified protein components, we have chosen a homolog of the Escherichia coli maltose/maltodextrin ATP-binding cassette transporter from Lactobacillus casei (MalE1-MalF1G1K12 ) as a model system. We show that the solute binding protein, MalE1, binds linear and cyclic maltodextrins but not maltose. Crystal structures of MalE1 complexed with these sugars provide a clue why maltose is not a substrate. P-Ser46-HPr inhibited MalE1/maltotetraose-stimulated ATPase activity of the transporter incorporated in proteoliposomes. Furthermore, cross-linking experiments revealed that P-Ser46-HPr contacts the nucleotide-binding subunit, MalK1, in proximity to the Walker A motif. However, P-Ser46-HPr did not block binding of ATP to MalK1. Together, our findings provide first biochemical evidence that P-Ser-HPr arrests the transport cycle by preventing ATP hydrolysis at the MalK1 subunits of the transporter.

-	~~Authors~~: ~~Homburg~~, C., Bommer, M., Wuttge, S., Hobe, C., Beck, S., Dobbek, H., Deutscher, J., Licht, A., Schneider, E.	+	Inducer exclusion in Firmicutes: insights into the regulation of a carbohydrate ATP binding cassette transporter from Lactobacillus casei BL23 by the signal transducing protein P-Ser46-HPr.,Homburg C, Bommer M, Wuttge S, Hobe C, Beck S, Dobbek H, Deutscher J, Licht A, Schneider E Mol Microbiol. 2017 Jul;105(1):25-45. doi: 10.1111/mmi.13680. Epub 2017 Apr 18. PMID:28370477<ref>PMID:28370477</ref>

-	~~Description: Maltodextrin binding protein MalE1 from L~~. ~~casei BL23 bound to beta-cyclodextrin~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Schneider, E]]~~	+	<div class="pdbe-citations 5mk9" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Dobbek, H~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacillus a von freudenreich 1890]]
	[[Category: Beck, S]]		[[Category: Beck, S]]
-	[[Category: Homburg, C]]
	[[Category: Bommer, M]]		[[Category: Bommer, M]]
-	[[Category: Hobe, C]]
	[[Category: Deutscher, J]]		[[Category: Deutscher, J]]
		+	[[Category: Dobbek, H]]
		+	[[Category: Hobe, C]]
		+	[[Category: Homburg, C]]
	[[Category: Licht, A]]		[[Category: Licht, A]]
		+	[[Category: Schneider, E]]
	[[Category: Wuttge, S]]		[[Category: Wuttge, S]]
		+	[[Category: Carbohydrate binding protein]]
		+	[[Category: Lactobacillus casei]]
		+	[[Category: Sugar binding protein]]

OCA at 11:23, 9 March 2017

OCA — Thu, 09 Mar 2017 11:23:32 GMT

←Older revision		Revision as of 11:23, 9 March 2017
Line 7:		Line 7:
	Description: Maltodextrin binding protein MalE1 from L. casei BL23 bound to beta-cyclodextrin		Description: Maltodextrin binding protein MalE1 from L. casei BL23 bound to beta-cyclodextrin
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Schneider, E]]
	[[Category: Dobbek, H]]		[[Category: Dobbek, H]]
		+	[[Category: Beck, S]]
		+	[[Category: Homburg, C]]
	[[Category: Bommer, M]]		[[Category: Bommer, M]]
		+	[[Category: Hobe, C]]
	[[Category: Deutscher, J]]		[[Category: Deutscher, J]]
	[[Category: Licht, A]]		[[Category: Licht, A]]
	[[Category: Wuttge, S]]		[[Category: Wuttge, S]]
-	[[Category: Schneider, E]]
-	[[Category: Beck, S]]
-	[[Category: Homburg, C]]
-	[[Category: Hobe, C]]

OCA at 20:13, 25 January 2017

OCA — Wed, 25 Jan 2017 20:13:33 GMT

←Older revision		Revision as of 20:13, 25 January 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5mk9 is ON HOLD	+	The entry 5mk9 is ON HOLD until Paper Publication

	Authors: Homburg, C., Bommer, M., Wuttge, S., Hobe, C., Beck, S., Dobbek, H., Deutscher, J., Licht, A., Schneider, E.		Authors: Homburg, C., Bommer, M., Wuttge, S., Hobe, C., Beck, S., Dobbek, H., Deutscher, J., Licht, A., Schneider, E.

OCA: Protected "5mk9" [edit=sysop:move=sysop]

OCA — Thu, 15 Dec 2016 16:23:49 GMT

Protected "5mk9" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:23, 15 December 2016

OCA: New page: '''Unreleased structure''' The entry 5mk9 is ON HOLD Authors: Homburg, C., Bommer, M., Wuttge, S., Hobe, C., Beck, S., Dobbek, H., Deutscher, J., Licht, A., Schneider, E. Description: ...

OCA — Thu, 15 Dec 2016 16:23:47 GMT

New page: '''Unreleased structure''' The entry 5mk9 is ON HOLD Authors: Homburg, C., Bommer, M., Wuttge, S., Hobe, C., Beck, S., Dobbek, H., Deutscher, J., Licht, A., Schneider, E. Description: ...

New page

'''Unreleased structure'''

The entry 5mk9 is ON HOLD

Authors: Homburg, C., Bommer, M., Wuttge, S., Hobe, C., Beck, S., Dobbek, H., Deutscher, J., Licht, A., Schneider, E.

Description: Maltodextrin binding protein MalE1 from L. casei BL23 bound to beta-cyclodextrin
[[Category: Unreleased Structures]]
[[Category: Dobbek, H]]
[[Category: Bommer, M]]
[[Category: Deutscher, J]]
[[Category: Licht, A]]
[[Category: Wuttge, S]]
[[Category: Schneider, E]]
[[Category: Beck, S]]
[[Category: Homburg, C]]
[[Category: Hobe, C]]