5mm8 - Revision history

OCA at 17:38, 8 November 2023

OCA — Wed, 08 Nov 2023 17:38:00 GMT

←Older revision		Revision as of 17:38, 8 November 2023
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	==Atomic resolution structure of SplE protease from Staphylococcus aureus==		==Atomic resolution structure of SplE protease from Staphylococcus aureus==
-	<StructureSection load='5mm8' size='340' side='right' caption='[[5mm8]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='5mm8' size='340' side='right'caption='[[5mm8]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mm8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MM8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MM8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mm8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MM8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=ACT:ACETATE+ION'>ACT</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>splE, SAUSA300_1754 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1280~~ "~~Micrococcus aureus~~" ~~(Rosenbach 1884) Zopf 1885])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mm8 OCA], [~~http~~://pdbe.org/5mm8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mm8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mm8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mm8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mm8 OCA], [https://pdbe.org/5mm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mm8 RCSB], [https://www.ebi.ac.uk/pdbsum/5mm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mm8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPLE_STAA8 SPLE_STAA8]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dubin, G]]~~	+	[[Category: Large Structures]]
-	~~[[Category: Stach, N]]~~	+
-	~~[[Category: Zdzalik, M]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protease~~]]	+
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: ~~Virulence~~]]	+	[[Category: Dubin G]]
		+	[[Category: Stach N]]
		+	[[Category: Zdzalik M]]

OCA at 14:05, 11 April 2018

OCA — Wed, 11 Apr 2018 14:05:49 GMT

←Older revision		Revision as of 14:05, 11 April 2018
Line 3:		Line 3:
	<StructureSection load='5mm8' size='340' side='right' caption='[[5mm8]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='5mm8' size='340' side='right' caption='[[5mm8]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mm8]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MM8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mm8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MM8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">splE, SAUSA300_1754 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mm8 OCA], [http://pdbe.org/5mm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mm8 RCSB], [http://www.ebi.ac.uk/pdbsum/5mm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mm8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mm8 OCA], [http://pdbe.org/5mm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mm8 RCSB], [http://www.ebi.ac.uk/pdbsum/5mm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mm8 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:28, 28 March 2018

OCA — Wed, 28 Mar 2018 06:28:48 GMT

←Older revision		Revision as of 06:28, 28 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mm8 is ~~ON HOLD~~	+	==Atomic resolution structure of SplE protease from Staphylococcus aureus==
		+	<StructureSection load='5mm8' size='340' side='right' caption='[[5mm8]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mm8]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MM8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MM8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mm8 OCA], [http://pdbe.org/5mm8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mm8 RCSB], [http://www.ebi.ac.uk/pdbsum/5mm8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mm8 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Staphylococcus aureus is a dangerous human pathogen characterized by alarmingly increasing antibiotic resistance. Accumulating evidence suggests the role of Spl proteases in staphylococcal virulence. Spl proteases have restricted, non-overlapping substrate specificity, suggesting that they may constitute a first example of a proteolytic system in bacteria. SplA, SplB, and SplD were previously characterized in terms of substrate specificity and structural determinants thereof. Here we analyze the substrate specificity of SplE documenting its unique P1 preference among Spl proteases and, in fact, among all chymotrypsin-like (family S1) proteases characterized to date. This is interesting since our understanding of the general aspects of proteolysis is based on seminal studies of S1 family members. To better understand the molecular determinants of the unusual specificity of SplE, the crystal structure of the protein is determined here. Conclusions from structural analysis are evaluated by successful grafting of SplE specificity on the scaffold of SplB protease.

-	~~Authors: Stach~~, N., Zdzalik, M., ~~Dubin~~, G.	+	Unique Substrate Specificity of SplE Serine Protease from Staphylococcus aureus.,Stach N, Kalinska M, Zdzalik M, Kitel R, Karim A, Serwin K, Rut W, Larsen K, Jabaiah A, Firlej M, Wladyka B, Daugherty P, Stennicke H, Drag M, Potempa J, Dubin G Structure. 2018 Mar 1. pii: S0969-2126(18)30048-0. doi:, 10.1016/j.str.2018.02.008. PMID:29526434<ref>PMID:29526434</ref>

-	~~Description: Atomic resolution structure~~ of ~~SplE protease from Staphylococcus aureus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Zdzalik, M]]~~	+	<div class="pdbe-citations 5mm8" style="background-color:#fffaf0;"></div>
-	~~[[Category: Stach, N]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Dubin, G]]		[[Category: Dubin, G]]
		+	[[Category: Stach, N]]
		+	[[Category: Zdzalik, M]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protease]]
		+	[[Category: Staphylococcus aureus]]
		+	[[Category: Virulence]]

OCA: Protected "5mm8" [edit=sysop:move=sysop]

OCA — Thu, 15 Dec 2016 16:29:39 GMT

Protected "5mm8" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:29, 15 December 2016

OCA: New page: '''Unreleased structure''' The entry 5mm8 is ON HOLD Authors: Stach, N., Zdzalik, M., Dubin, G. Description: Atomic resolution structure of SplE protease from Staphylococcus aureus [[C...

OCA — Thu, 15 Dec 2016 16:29:37 GMT

New page: '''Unreleased structure''' The entry 5mm8 is ON HOLD Authors: Stach, N., Zdzalik, M., Dubin, G. Description: Atomic resolution structure of SplE protease from Staphylococcus aureus [[C...

New page

'''Unreleased structure'''

The entry 5mm8 is ON HOLD

Authors: Stach, N., Zdzalik, M., Dubin, G.

Description: Atomic resolution structure of SplE protease from Staphylococcus aureus
[[Category: Unreleased Structures]]
[[Category: Zdzalik, M]]
[[Category: Stach, N]]
[[Category: Dubin, G]]