5mns - Revision history

OCA at 17:41, 8 November 2023

OCA — Wed, 08 Nov 2023 17:41:31 GMT

←Older revision		Revision as of 17:41, 8 November 2023
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	==Structural and functional characterization of OleP in complex with 6DEB in sodium formate==		==Structural and functional characterization of OleP in complex with 6DEB in sodium formate==
-	<StructureSection load='5mns' size='340' side='right' caption='[[5mns]], [[Resolution\|resolution]] 2.62Å' scene=''>	+	<StructureSection load='5mns' size='340' side='right'caption='[[5mns]], [[Resolution\|resolution]] 2.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mns]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"actinomyces_antibioticus"_waksman_and_woodruff_1941 "actinomyces~~ antibioticus~~" waksman and woodruff 1941~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MNS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MNS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mns]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MNS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=DEB:6~~-~~DEOXYERYTHRONOLIDE+B'>DEB</scene>~~, ~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.62Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~oleP ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=1890 "Actinomyces antibioticus" Waksman and Woodruff 1941])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DEB:6-DEOXYERYTHRONOLIDE+B'>DEB</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mns OCA], [~~http~~://pdbe.org/5mns PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mns RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mns PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mns ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mns OCA], [https://pdbe.org/5mns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mns RCSB], [https://www.ebi.ac.uk/pdbsum/5mns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mns ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q59819_STRAT Q59819_STRAT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Actinomyces~~ antibioticus ~~waksman and woodruff 1941~~]]	+	[[Category: Large Structures]]
-	[[Category: Montemiglio~~, L C~~]]	+	[[Category: Streptomyces antibioticus]]
-	[[Category: Parisi, G]]	+	[[Category: Montemiglio LC]]
-	[[Category: Savino, C]]	+	[[Category: Parisi G]]
-	[[Category: Vallone, B]]	+	[[Category: Savino C]]
-	~~[[Category: Cytochrome]]~~	+	[[Category: Vallone B]]
-	~~[[Category: Epoxidase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: P450~~]]	+

OCA at 08:49, 14 November 2018

OCA — Wed, 14 Nov 2018 08:49:46 GMT

←Older revision		Revision as of 08:49, 14 November 2018
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	<StructureSection load='5mns' size='340' side='right' caption='[[5mns]], [[Resolution\|resolution]] 2.62Å' scene=''>		<StructureSection load='5mns' size='340' side='right' caption='[[5mns]], [[Resolution\|resolution]] 2.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mns]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MNS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mns]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_antibioticus"_waksman_and_woodruff_1941 "actinomyces antibioticus" waksman and woodruff 1941]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MNS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DEB:6-DEOXYERYTHRONOLIDE+B'>DEB</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DEB:6-DEOXYERYTHRONOLIDE+B'>DEB</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">oleP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1890 "Actinomyces antibioticus" Waksman and Woodruff 1941])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mns OCA], [http://pdbe.org/5mns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mns RCSB], [http://www.ebi.ac.uk/pdbsum/5mns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mns ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mns OCA], [http://pdbe.org/5mns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mns RCSB], [http://www.ebi.ac.uk/pdbsum/5mns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mns ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The regulation of cytochrome P450 activity is often achieved by structural transitions induced by substrate binding. We describe the conformational transition experienced upon binding by the P450 OleP, an epoxygenase involved in oleandomycin biosynthesis. OleP bound to the substrate analog 6DEB crystallized in 2 forms: one with an ensemble of open and closed conformations in the asymmetric unit and another with only the closed conformation. Characterization of OleP-6DEB binding kinetics, also using the P450 inhibitor clotrimazole, unveiled a complex binding mechanism that involves slow conformational rearrangement with the accumulation of a spectroscopically detectable intermediate where 6DEB is bound to open OleP. Data reported herein provide structural snapshots of key precatalytic steps in the OleP reaction and explain how structural rearrangements induced by substrate binding regulate activity.-Parisi, G., Montemiglio, L. C., Giuffre, A., Macone, A., Scaglione, A., Cerutti, G., Exertier, C., Savino, C., Vallone, B. Substrate-induced conformational change in cytochrome P450 OleP.
		+
		+	Substrate-induced conformational change in cytochrome P450 OleP.,Parisi G, Montemiglio LC, Giuffre A, Macone A, Scaglione A, Cerutti G, Exertier C, Savino C, Vallone B FASEB J. 2018 Sep 12:fj201800450RR. doi: 10.1096/fj.201800450RR. PMID:30207799<ref>PMID:30207799</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5mns" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actinomyces antibioticus waksman and woodruff 1941]]
	[[Category: Montemiglio, L C]]		[[Category: Montemiglio, L C]]
	[[Category: Parisi, G]]		[[Category: Parisi, G]]

OCA at 06:13, 28 February 2018

OCA — Wed, 28 Feb 2018 06:13:00 GMT

←Older revision		Revision as of 06:13, 28 February 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5mns is ~~ON HOLD~~	+	==Structural and functional characterization of OleP in complex with 6DEB in sodium formate==
-		+	<StructureSection load='5mns' size='340' side='right' caption='[[5mns]], [[Resolution\|resolution]] 2.62Å' scene=''>
-	~~Authors~~: ~~Parisi~~, G., ~~Savino~~, C., ~~Montemiglio, L~~.C., ~~Vallone, B~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5mns]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MNS FirstGlance]. <br>
-	~~Description~~: ~~Structural and functional characterization of OleP in complex with 6DEB in sodium formate~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DEB:6-DEOXYERYTHRONOLIDE+B'>DEB</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mns OCA], [http://pdbe.org/5mns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mns RCSB], [http://www.ebi.ac.uk/pdbsum/5mns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mns ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Montemiglio, L C]]
		+	[[Category: Parisi, G]]
	[[Category: Savino, C]]		[[Category: Savino, C]]
	[[Category: Vallone, B]]		[[Category: Vallone, B]]
-	[[Category: ~~Parisi, G~~]]	+	[[Category: Cytochrome]]
-	[[Category: ~~Montemiglio, L.C~~]]	+	[[Category: Epoxidase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: P450]]

OCA at 05:28, 17 January 2018

OCA — Wed, 17 Jan 2018 05:28:16 GMT

←Older revision		Revision as of 05:28, 17 January 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5mns is ON HOLD ~~until Paper Publication~~	+	The entry 5mns is ON HOLD

	Authors: Parisi, G., Savino, C., Montemiglio, L.C., Vallone, B.		Authors: Parisi, G., Savino, C., Montemiglio, L.C., Vallone, B.
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	Description: Structural and functional characterization of OleP in complex with 6DEB in sodium formate		Description: Structural and functional characterization of OleP in complex with 6DEB in sodium formate
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Montemiglio, L.C]]
	[[Category: Savino, C]]		[[Category: Savino, C]]
	[[Category: Vallone, B]]		[[Category: Vallone, B]]
	[[Category: Parisi, G]]		[[Category: Parisi, G]]
		+	[[Category: Montemiglio, L.C]]

OCA: Protected "5mns" [edit=sysop:move=sysop]

OCA — Thu, 22 Dec 2016 19:13:30 GMT

Protected "5mns" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:13, 22 December 2016

OCA: New page: '''Unreleased structure''' The entry 5mns is ON HOLD until Paper Publication Authors: Parisi, G., Savino, C., Montemiglio, L.C., Vallone, B. Description: Structural and functional char...

OCA — Thu, 22 Dec 2016 19:13:29 GMT

New page: '''Unreleased structure''' The entry 5mns is ON HOLD until Paper Publication Authors: Parisi, G., Savino, C., Montemiglio, L.C., Vallone, B. Description: Structural and functional char...

New page

'''Unreleased structure'''

The entry 5mns is ON HOLD until Paper Publication

Authors: Parisi, G., Savino, C., Montemiglio, L.C., Vallone, B.

Description: Structural and functional characterization of OleP in complex with 6DEB in sodium formate
[[Category: Unreleased Structures]]
[[Category: Montemiglio, L.C]]
[[Category: Savino, C]]
[[Category: Vallone, B]]
[[Category: Parisi, G]]