5mqj - Revision history

OCA at 17:44, 8 November 2023

OCA — Wed, 08 Nov 2023 17:44:31 GMT

←Older revision		Revision as of 17:44, 8 November 2023
Line 3:		Line 3:
	<StructureSection load='5mqj' size='340' side='right'caption='[[5mqj]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='5mqj' size='340' side='right'caption='[[5mqj]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mqj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MQJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5MQJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mqj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MQJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.7Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxycytidine_kinase Deoxycytidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.74 2.7.1.74] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5mqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mqj OCA], [~~http~~://pdbe.org/5mqj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mqj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mqj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mqj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mqj OCA], [https://pdbe.org/5mqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mqj RCSB], [https://www.ebi.ac.uk/pdbsum/5mqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mqj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DCK_HUMAN DCK_HUMAN]] Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.<ref>PMID:18377927</ref> <ref>PMID:20614893</ref>	+	[https://www.uniprot.org/uniprot/DCK_HUMAN DCK_HUMAN] Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.<ref>PMID:18377927</ref> <ref>PMID:20614893</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Deoxycytidine kinase~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Audebert, S]]	+	[[Category: Audebert S]]
-	[[Category: Baudelet, E]]	+	[[Category: Baudelet E]]
-	[[Category: Betzi, S]]	+	[[Category: Betzi S]]
-	[[Category: Combes, S]]	+	[[Category: Combes S]]
-	[[Category: Dubreuil, P]]	+	[[Category: Dubreuil P]]
-	[[Category: Gros, L]]	+	[[Category: Gros L]]
-	[[Category: Hajem, B]]	+	[[Category: Hajem B]]
-	[[Category: Hammam, K]]	+	[[Category: Hammam K]]
-	[[Category: Humbert, M]]	+	[[Category: Humbert M]]
-	[[Category: Letard, S]]	+	[[Category: Letard S]]
-	[[Category: Lopez, S]]	+	[[Category: Lopez S]]
-	[[Category: Lugari, A]]	+	[[Category: Lugari A]]
-	[[Category: Mansfield, C]]	+	[[Category: Mansfield C]]
-	[[Category: Morelli, X]]	+	[[Category: Morelli X]]
-	[[Category: Moussy, A]]	+	[[Category: Moussy A]]
-	[[Category: Pez, D]]	+	[[Category: Pez D]]
-	[[Category: Rebuffet, E]]	+	[[Category: Rebuffet E]]
-	[[Category: Saez-Ayala, M]]	+	[[Category: Saez-Ayala M]]
-	[[Category: Sepulveda, P ~~de]]~~	+	[[Category: De Sepulveda P]]
-	~~[[Category: Dck]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:25, 19 August 2020

OCA — Wed, 19 Aug 2020 07:25:59 GMT

←Older revision		Revision as of 07:25, 19 August 2020
Line 1:		Line 1:

	==Crystal structure of dCK mutant C3S==		==Crystal structure of dCK mutant C3S==
-	<StructureSection load='5mqj' size='340' side='right' caption='[[5mqj]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<StructureSection load='5mqj' size='340' side='right'caption='[[5mqj]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mqj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MQJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MQJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mqj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MQJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MQJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxycytidine_kinase Deoxycytidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.74 2.7.1.74] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxycytidine_kinase Deoxycytidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.74 2.7.1.74] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mqj OCA], [http://pdbe.org/5mqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mqj RCSB], [http://www.ebi.ac.uk/pdbsum/5mqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mqj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mqj OCA], [http://pdbe.org/5mqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mqj RCSB], [http://www.ebi.ac.uk/pdbsum/5mqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mqj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5mqj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5mqj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Deoxycytidine kinase 3D structures\|Deoxycytidine kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Deoxycytidine kinase]]		[[Category: Deoxycytidine kinase]]
		+	[[Category: Large Structures]]
	[[Category: Audebert, S]]		[[Category: Audebert, S]]
	[[Category: Baudelet, E]]		[[Category: Baudelet, E]]

OCA at 07:37, 22 November 2017

OCA — Wed, 22 Nov 2017 07:37:41 GMT

←Older revision		Revision as of 07:37, 22 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mqj is ~~ON HOLD~~	+	==Crystal structure of dCK mutant C3S==
		+	<StructureSection load='5mqj' size='340' side='right' caption='[[5mqj]], [[Resolution\|resolution]] 3.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mqj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MQJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxycytidine_kinase Deoxycytidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.74 2.7.1.74] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mqj OCA], [http://pdbe.org/5mqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mqj RCSB], [http://www.ebi.ac.uk/pdbsum/5mqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mqj ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DCK_HUMAN DCK_HUMAN]] Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.<ref>PMID:18377927</ref> <ref>PMID:20614893</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Masitinib, a highly selective protein kinase inhibitor, can sensitise gemcitabine-refractory cancer cell lines when used in combination with gemcitabine. Here we report a reverse proteomic approach that identifies the target responsible for this sensitisation: the deoxycytidine kinase (dCK). Masitinib, as well as other protein kinase inhibitors, such as imatinib, interact with dCK and provoke an unforeseen conformational-dependent activation of this nucleoside kinase, modulating phosphorylation of nucleoside analogue drugs. This phenomenon leads to an increase of prodrug phosphorylation of most of the chemotherapeutic drugs activated by this nucleoside kinase. The unforeseen dual activity of protein kinase inhibition/nucleoside kinase activation could be of great therapeutic benefit, through either reducing toxicity of therapeutic agents by maintaining effectiveness at lower doses or by counteracting drug resistance initiated via down modulation of dCK target.

-	~~Authors:~~ Saez-Ayala, M., Rebuffet, E~~., Hammam, K.~~, Gros, L., Lopez, S., Hajem, B., Humbert, M., Baudelet, E., Audebert, S., Betzi, S., Lugari, A., Combes, S~~., Pez, D.~~, Letard, S., Mansfield, C., Moussy, A., de Sepulveda, P., Morelli, X., Dubreuil, P.	+	Dual protein kinase and nucleoside kinase modulators for rationally designed polypharmacology.,Hammam K, Saez-Ayala M, Rebuffet E, Gros L, Lopez S, Hajem B, Humbert M, Baudelet E, Audebert S, Betzi S, Lugari A, Combes S, Letard S, Casteran N, Mansfield C, Moussy A, De Sepulveda P, Morelli X, Dubreuil P Nat Commun. 2017 Nov 10;8(1):1420. doi: 10.1038/s41467-017-01582-5. PMID:29127277<ref>PMID:29127277</ref>

-	~~Description: Crystal structure~~ of ~~dCK mutant C3S~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Dubreuil~~, P]]	+	<div class="pdbe-citations 5mqj" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Mansfield~~, C]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Deoxycytidine kinase]]
		+	[[Category: Audebert, S]]
		+	[[Category: Baudelet, E]]
		+	[[Category: Betzi, S]]
	[[Category: Combes, S]]		[[Category: Combes, S]]
		+	[[Category: Dubreuil, P]]
	[[Category: Gros, L]]		[[Category: Gros, L]]
-	[[Category: Humbert, M]]
-	[[Category: Rebuffet, E]]
-	[[Category: Moussy, A]]
-	[[Category: Baudelet, E]]
	[[Category: Hajem, B]]		[[Category: Hajem, B]]
		+	[[Category: Hammam, K]]
		+	[[Category: Humbert, M]]
	[[Category: Letard, S]]		[[Category: Letard, S]]
		+	[[Category: Lopez, S]]
	[[Category: Lugari, A]]		[[Category: Lugari, A]]
		+	[[Category: Mansfield, C]]
	[[Category: Morelli, X]]		[[Category: Morelli, X]]
-	[[Category: ~~Betzi~~, ~~S]]~~	+	[[Category: Moussy, A]]
-	~~[[Category: De Sepulveda, P~~]]	+
	[[Category: Pez, D]]		[[Category: Pez, D]]
-	[[Category: ~~Audebert~~, ~~S]]~~	+	[[Category: Rebuffet, E]]
-	~~[[Category: Lopez, S~~]]	+
	[[Category: Saez-Ayala, M]]		[[Category: Saez-Ayala, M]]
-	[[Category: ~~Hammam~~, K]]	+	[[Category: Sepulveda, P de]]
		+	[[Category: Dck]]
		+	[[Category: Transferase]]

OCA at 11:33, 9 March 2017

OCA — Thu, 09 Mar 2017 11:33:01 GMT

←Older revision		Revision as of 11:33, 9 March 2017
Line 7:		Line 7:
	Description: Crystal structure of dCK mutant C3S		Description: Crystal structure of dCK mutant C3S
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Combes, S]]
-	[[Category: Gros, L]]
-	[[Category: Baudelet, E]]
-	[[Category: Letard, S]]
-	[[Category: Morelli, X]]
-	[[Category: Betzi, S]]
-	[[Category: Pez, D]]
	[[Category: Dubreuil, P]]		[[Category: Dubreuil, P]]
	[[Category: Mansfield, C]]		[[Category: Mansfield, C]]
		+	[[Category: Combes, S]]
		+	[[Category: Gros, L]]
	[[Category: Humbert, M]]		[[Category: Humbert, M]]
	[[Category: Rebuffet, E]]		[[Category: Rebuffet, E]]
	[[Category: Moussy, A]]		[[Category: Moussy, A]]
		+	[[Category: Baudelet, E]]
	[[Category: Hajem, B]]		[[Category: Hajem, B]]
		+	[[Category: Letard, S]]
	[[Category: Lugari, A]]		[[Category: Lugari, A]]
		+	[[Category: Morelli, X]]
		+	[[Category: Betzi, S]]
	[[Category: De Sepulveda, P]]		[[Category: De Sepulveda, P]]
		+	[[Category: Pez, D]]
	[[Category: Audebert, S]]		[[Category: Audebert, S]]
	[[Category: Lopez, S]]		[[Category: Lopez, S]]
	[[Category: Saez-Ayala, M]]		[[Category: Saez-Ayala, M]]
	[[Category: Hammam, K]]		[[Category: Hammam, K]]

OCA at 20:24, 25 January 2017

OCA — Wed, 25 Jan 2017 20:24:35 GMT

←Older revision		Revision as of 20:24, 25 January 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5mqj is ON HOLD ~~until sometime in the future~~	+	The entry 5mqj is ON HOLD

	Authors: Saez-Ayala, M., Rebuffet, E., Hammam, K., Gros, L., Lopez, S., Hajem, B., Humbert, M., Baudelet, E., Audebert, S., Betzi, S., Lugari, A., Combes, S., Pez, D., Letard, S., Mansfield, C., Moussy, A., de Sepulveda, P., Morelli, X., Dubreuil, P.		Authors: Saez-Ayala, M., Rebuffet, E., Hammam, K., Gros, L., Lopez, S., Hajem, B., Humbert, M., Baudelet, E., Audebert, S., Betzi, S., Lugari, A., Combes, S., Pez, D., Letard, S., Mansfield, C., Moussy, A., de Sepulveda, P., Morelli, X., Dubreuil, P.

OCA: Protected "5mqj" [edit=sysop:move=sysop]

OCA — Mon, 02 Jan 2017 13:52:22 GMT

Protected "5mqj" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:52, 2 January 2017

OCA: New page: '''Unreleased structure''' The entry 5mqj is ON HOLD until sometime in the future Authors: Saez-Ayala, M., Rebuffet, E., Hammam, K., Gros, L., Lopez, S., Hajem, B., Humbert, M., Baudele...

OCA — Mon, 02 Jan 2017 13:52:20 GMT

New page: '''Unreleased structure''' The entry 5mqj is ON HOLD until sometime in the future Authors: Saez-Ayala, M., Rebuffet, E., Hammam, K., Gros, L., Lopez, S., Hajem, B., Humbert, M., Baudele...

New page

'''Unreleased structure'''

The entry 5mqj is ON HOLD until sometime in the future

Authors: Saez-Ayala, M., Rebuffet, E., Hammam, K., Gros, L., Lopez, S., Hajem, B., Humbert, M., Baudelet, E., Audebert, S., Betzi, S., Lugari, A., Combes, S., Pez, D., Letard, S., Mansfield, C., Moussy, A., de Sepulveda, P., Morelli, X., Dubreuil, P.

Description: Crystal structure of dCK mutant C3S
[[Category: Unreleased Structures]]
[[Category: Combes, S]]
[[Category: Gros, L]]
[[Category: Baudelet, E]]
[[Category: Letard, S]]
[[Category: Morelli, X]]
[[Category: Betzi, S]]
[[Category: Pez, D]]
[[Category: Dubreuil, P]]
[[Category: Mansfield, C]]
[[Category: Humbert, M]]
[[Category: Rebuffet, E]]
[[Category: Moussy, A]]
[[Category: Hajem, B]]
[[Category: Lugari, A]]
[[Category: De Sepulveda, P]]
[[Category: Audebert, S]]
[[Category: Lopez, S]]
[[Category: Saez-Ayala, M]]
[[Category: Hammam, K]]