5mr6 - Revision history

OCA at 17:46, 8 November 2023

OCA — Wed, 08 Nov 2023 17:46:44 GMT

←Older revision		Revision as of 17:46, 8 November 2023
Line 1:		Line 1:

	==XiaF from Streptomyces sp. in complex with FADH2 and Glycerol==		==XiaF from Streptomyces sp. in complex with FADH2 and Glycerol==
-	<StructureSection load='5mr6' size='340' side='right' caption='[[5mr6]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5mr6' size='340' side='right'caption='[[5mr6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mr6]] is a 24 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chainia_sp~~. ~~Chainia~~ sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MR6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MR6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mr6]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MR6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[2jbr\|2jbr]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">xiaF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Chainia sp.])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mr6 OCA], [https://pdbe.org/5mr6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mr6 RCSB], [https://www.ebi.ac.uk/pdbsum/5mr6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mr6 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mr6 OCA], [~~http~~://pdbe.org/5mr6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mr6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mr6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mr6 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/I7IIA9_9ACTN I7IIA9_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chainia~~ sp]]	+	[[Category: Large Structures]]
-	[[Category: Baer, P]]	+	[[Category: Streptomyces sp]]
-	[[Category: Baunach, M]]	+	[[Category: Baer P]]
-	[[Category: Groll, M]]	+	[[Category: Baunach M]]
-	[[Category: Hertweck, C]]	+	[[Category: Groll M]]
-	[[Category: Ishida-Ito, M]]	+	[[Category: Hertweck C]]
-	[[Category: Kugel, S]]	+	[[Category: Ishida-Ito M]]
-	[[Category: Sundaram, S]]	+	[[Category: Kugel S]]
-	[[Category: Xu, Z]]	+	[[Category: Sundaram S]]
-	~~[[Category: Detoxification]]~~	+	[[Category: Xu Z]]
-	~~[[Category: Indigo]]~~	+
-	~~[[Category: Indirubin]]~~	+
-	~~[[Category: Non-canonical flavin-dependent terpenoid cyclase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Xenobiotics-degrading enzyme~~]]	+

OCA at 11:02, 6 November 2017

OCA — Mon, 06 Nov 2017 11:02:18 GMT

←Older revision		Revision as of 11:02, 6 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mr6 is ~~ON HOLD until Paper~~ Publication	+	==XiaF from Streptomyces sp. in complex with FADH2 and Glycerol==
		+	<StructureSection load='5mr6' size='340' side='right' caption='[[5mr6]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mr6]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Chainia_sp. Chainia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MR6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MR6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jbr\|2jbr]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xiaF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Chainia sp.])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mr6 OCA], [http://pdbe.org/5mr6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mr6 RCSB], [http://www.ebi.ac.uk/pdbsum/5mr6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mr6 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Terpenoid natural products comprise a wide range of molecular architectures that typically result from C-C bond formations catalysed by classical type I/II terpene cyclases. However, the molecular diversity of biologically active terpenoids is substantially increased by fully unrelated, non-canonical terpenoid cyclases. Their evolutionary origin has remained enigmatic. Here we report the in vitro reconstitution of an unusual flavin-dependent bacterial indoloterpenoid cyclase, XiaF, together with a designated flavoenzyme-reductase (XiaP) that mediates a key step in xiamycin biosynthesis. The crystal structure of XiaF with bound FADH2 (at 2.4 A resolution) and phylogenetic analyses reveal that XiaF is, surprisingly, most closely related to xenobiotic-degrading enzymes. Biotransformation assays show that XiaF is a designated indole hydroxylase that can be used for the production of indigo and indirubin. We unveil a cryptic hydroxylation step that sets the basis for terpenoid cyclization and suggest that the cyclase has evolved from xenobiotics detoxification enzymes.

-	~~Authors: Kugel~~, S., Baunach, M., Baer, P., Ishida-Ito, M., Sundaram, S., Xu, Z., Groll, M., Hertweck, C.	+	Cryptic indole hydroxylation by a non-canonical terpenoid cyclase parallels bacterial xenobiotic detoxification.,Kugel S, Baunach M, Baer P, Ishida-Ito M, Sundaram S, Xu Z, Groll M, Hertweck C Nat Commun. 2017 Jun 15;8:15804. doi: 10.1038/ncomms15804. PMID:28643772<ref>PMID:28643772</ref>

-	~~Description: XiaF from Streptomyces sp~~. ~~in complex with FADH2 and Glycerol~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Hertweck, C]]~~	+	<div class="pdbe-citations 5mr6" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Sundaram, S~~]]	+	== References ==
-	[[Category: ~~Kugel~~, S]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chainia sp]]
		+	[[Category: Baer, P]]
	[[Category: Baunach, M]]		[[Category: Baunach, M]]
		+	[[Category: Groll, M]]
		+	[[Category: Hertweck, C]]
	[[Category: Ishida-Ito, M]]		[[Category: Ishida-Ito, M]]
		+	[[Category: Kugel, S]]
		+	[[Category: Sundaram, S]]
	[[Category: Xu, Z]]		[[Category: Xu, Z]]
-	[[Category: ~~Groll, M~~]]	+	[[Category: Detoxification]]
-	[[Category: ~~Baer, P~~]]	+	[[Category: Indigo]]
		+	[[Category: Indirubin]]
		+	[[Category: Non-canonical flavin-dependent terpenoid cyclase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Xenobiotics-degrading enzyme]]

OCA at 11:34, 9 March 2017

OCA — Thu, 09 Mar 2017 11:34:25 GMT

←Older revision		Revision as of 11:34, 9 March 2017
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Hertweck, C]]		[[Category: Hertweck, C]]
		+	[[Category: Sundaram, S]]
	[[Category: Kugel, S]]		[[Category: Kugel, S]]
	[[Category: Baunach, M]]		[[Category: Baunach, M]]
	[[Category: Ishida-Ito, M]]		[[Category: Ishida-Ito, M]]
-	[[Category: Baer, P]]
-	[[Category: Sundaram, S]]
	[[Category: Xu, Z]]		[[Category: Xu, Z]]
	[[Category: Groll, M]]		[[Category: Groll, M]]
		+	[[Category: Baer, P]]

OCA at 14:17, 4 January 2017

OCA — Wed, 04 Jan 2017 14:17:38 GMT

←Older revision		Revision as of 14:17, 4 January 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5mr6 is ON HOLD	+	The entry 5mr6 is ON HOLD until Paper Publication

	Authors: Kugel, S., Baunach, M., Baer, P., Ishida-Ito, M., Sundaram, S., Xu, Z., Groll, M., Hertweck, C.		Authors: Kugel, S., Baunach, M., Baer, P., Ishida-Ito, M., Sundaram, S., Xu, Z., Groll, M., Hertweck, C.

OCA: Protected "5mr6" [edit=sysop:move=sysop]

OCA — Mon, 02 Jan 2017 13:55:27 GMT

Protected "5mr6" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:55, 2 January 2017

OCA: New page: '''Unreleased structure''' The entry 5mr6 is ON HOLD Authors: Kugel, S., Baunach, M., Baer, P., Ishida-Ito, M., Sundaram, S., Xu, Z., Groll, M., Hertweck, C. Description: XiaF from Str...

OCA — Mon, 02 Jan 2017 13:55:25 GMT

New page: '''Unreleased structure''' The entry 5mr6 is ON HOLD Authors: Kugel, S., Baunach, M., Baer, P., Ishida-Ito, M., Sundaram, S., Xu, Z., Groll, M., Hertweck, C. Description: XiaF from Str...

New page

'''Unreleased structure'''

The entry 5mr6 is ON HOLD

Authors: Kugel, S., Baunach, M., Baer, P., Ishida-Ito, M., Sundaram, S., Xu, Z., Groll, M., Hertweck, C.

Description: XiaF from Streptomyces sp. in complex with FADH2 and Glycerol
[[Category: Unreleased Structures]]
[[Category: Hertweck, C]]
[[Category: Kugel, S]]
[[Category: Baunach, M]]
[[Category: Ishida-Ito, M]]
[[Category: Baer, P]]
[[Category: Sundaram, S]]
[[Category: Xu, Z]]
[[Category: Groll, M]]