5msn - Revision history

OCA at 17:48, 8 November 2023

OCA — Wed, 08 Nov 2023 17:48:20 GMT

←Older revision		Revision as of 17:48, 8 November 2023
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	<StructureSection load='5msn' size='340' side='right'caption='[[5msn]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5msn' size='340' side='right'caption='[[5msn]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5msn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MSN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5MSN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5msn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MSN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MSN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5msn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5msn OCA], [~~http~~://pdbe.org/5msn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5msn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5msn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5msn ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.002Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5msn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5msn OCA], [https://pdbe.org/5msn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5msn RCSB], [https://www.ebi.ac.uk/pdbsum/5msn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5msn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DCC1_YEAST DCC1_YEAST]] Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA.<ref>PMID:11389843</ref> <ref>PMID:15964801</ref>	+	[https://www.uniprot.org/uniprot/DCC1_YEAST DCC1_YEAST] Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA.<ref>PMID:11389843</ref> <ref>PMID:15964801</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Singleton, M R~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Wade, B O~~]]	+	[[Category: Singleton MR]]
-	[[Category: ~~Cell cycle]]~~	+	[[Category: Wade BO]]
-	~~[[Category: Dna repair]]~~	+
-	~~[[Category: Winged-helix~~]]	+

OCA at 07:28, 19 August 2020

OCA — Wed, 19 Aug 2020 07:28:02 GMT

←Older revision		Revision as of 07:28, 19 August 2020
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	==Structure of the Dcc1 Protein==		==Structure of the Dcc1 Protein==
-	<StructureSection load='5msn' size='340' side='right' caption='[[5msn]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5msn' size='340' side='right'caption='[[5msn]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5msn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MSN OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MSN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5msn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MSN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MSN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5msn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5msn OCA], [http://pdbe.org/5msn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5msn RCSB], [http://www.ebi.ac.uk/pdbsum/5msn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5msn ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5msn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5msn OCA], [http://pdbe.org/5msn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5msn RCSB], [http://www.ebi.ac.uk/pdbsum/5msn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5msn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Singleton, M R]]		[[Category: Singleton, M R]]
	[[Category: Wade, B O]]		[[Category: Wade, B O]]

OCA at 07:28, 9 March 2017

OCA — Thu, 09 Mar 2017 07:28:09 GMT

←Older revision		Revision as of 07:28, 9 March 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5msn is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structure of the Dcc1 Protein==
		+	<StructureSection load='5msn' size='340' side='right' caption='[[5msn]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5msn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MSN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MSN FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5msn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5msn OCA], [http://pdbe.org/5msn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5msn RCSB], [http://www.ebi.ac.uk/pdbsum/5msn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5msn ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DCC1_YEAST DCC1_YEAST]] Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA.<ref>PMID:11389843</ref> <ref>PMID:15964801</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Replication factor C complexes load and unload processivity clamps from DNA and are involved in multiple DNA replication and repair pathways. The RFCCtf18 variant complex is required for activation of the intra-S-phase checkpoint at stalled replication forks and aids the establishment of sister chromatid cohesion. Unlike other RFC complexes, RFCCtf18 contains two non-Rfc subunits, Dcc1 and Ctf8. Here, we present the crystal structure of the Dcc1-Ctf8 heterodimer bound to the C-terminus of Ctf18. We find that the C-terminus of Dcc1 contains three-winged helix domains, which bind to both ssDNA and dsDNA We further show that these domains are required for full recruitment of the complex to chromatin, and correct activation of the replication checkpoint. These findings provide the first structural data on a eukaryotic seven-subunit clamp loader and define a new biochemical activity for Dcc1.

-	~~Authors~~:	+	Structural studies of RFCCtf18 reveal a novel chromatin recruitment role for Dcc1.,Wade BO, Liu HW, Samora CP, Uhlmann F, Singleton MR EMBO Rep. 2017 Feb 10. pii: e201642825. doi: 10.15252/embr.201642825. PMID:28188145<ref>PMID:28188145</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5msn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Singleton, M R]]
		+	[[Category: Wade, B O]]
		+	[[Category: Cell cycle]]
		+	[[Category: Dna repair]]
		+	[[Category: Winged-helix]]

OCA: Protected "5msn" [edit=sysop:move=sysop]

OCA — Wed, 18 Jan 2017 13:09:49 GMT

Protected "5msn" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:09, 18 January 2017

OCA: New page: '''Unreleased structure''' The entry 5msn is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 18 Jan 2017 13:09:48 GMT

New page: '''Unreleased structure''' The entry 5msn is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5msn is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]