5mu4 - Revision history

OCA at 17:49, 8 November 2023

OCA — Wed, 08 Nov 2023 17:49:52 GMT

←Older revision		Revision as of 17:49, 8 November 2023
Line 3:		Line 3:
	<StructureSection load='5mu4' size='340' side='right'caption='[[5mu4]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5mu4' size='340' side='right'caption='[[5mu4]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mu4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MU4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5MU4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mu4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_phage_KP32 Klebsiella phage KP32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MU4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5mu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mu4 OCA], [~~http~~://pdbe.org/5mu4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mu4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mu4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mu4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mu4 OCA], [https://pdbe.org/5mu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mu4 RCSB], [https://www.ebi.ac.uk/pdbsum/5mu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mu4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D1L2W4_9CAUD D1L2W4_9CAUD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Klebsiella phage KP32]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brzozowska, E]]	+	[[Category: Brzozowska E]]
-	[[Category: Dauter, M]]	+	[[Category: Dauter M]]
-	[[Category: Dauter, Z]]	+	[[Category: Dauter Z]]
-	[[Category: Gamian, A]]	+	[[Category: Gamian A]]
-	[[Category: Pawlik, K]]	+	[[Category: Pawlik K]]
-	[[Category: Pyra, A]]	+	[[Category: Pyra A]]
-	~~[[Category: Antibacterial activity]]~~	+
-	~~[[Category: Bacteriophage]]~~	+
-	~~[[Category: Exopolysaccharide depolymerase]]~~	+
-	~~[[Category: Tail tubular protein]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 07:29, 19 August 2020

OCA — Wed, 19 Aug 2020 07:29:07 GMT

←Older revision		Revision as of 07:29, 19 August 2020
Line 1:		Line 1:

	==Tail Tubular Protein A of Klebsiella pneumoniae bacteriophage KP32==		==Tail Tubular Protein A of Klebsiella pneumoniae bacteriophage KP32==
-	<StructureSection load='5mu4' size='340' side='right' caption='[[5mu4]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5mu4' size='340' side='right'caption='[[5mu4]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mu4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MU4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MU4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mu4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MU4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MU4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mu4 OCA], [http://pdbe.org/5mu4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mu4 RCSB], [http://www.ebi.ac.uk/pdbsum/5mu4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mu4 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mu4 OCA], [http://pdbe.org/5mu4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mu4 RCSB], [http://www.ebi.ac.uk/pdbsum/5mu4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mu4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Brzozowska, E]]		[[Category: Brzozowska, E]]
	[[Category: Dauter, M]]		[[Category: Dauter, M]]

OCA at 09:44, 3 August 2017

OCA — Thu, 03 Aug 2017 09:44:24 GMT

←Older revision		Revision as of 09:44, 3 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mu4 is ~~ON HOLD~~	+	==Tail Tubular Protein A of Klebsiella pneumoniae bacteriophage KP32==
		+	<StructureSection load='5mu4' size='340' side='right' caption='[[5mu4]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mu4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MU4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MU4 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mu4 OCA], [http://pdbe.org/5mu4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mu4 RCSB], [http://www.ebi.ac.uk/pdbsum/5mu4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mu4 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tail tubular protein A (TTPA) is a structural tail protein of Klebsiella pneumoniae bacteriophage KP32, and is responsible for adhering the bacteriophage to host cells. For the first time, we found that TTPA also exhibits lytic activity towards capsular exopolysaccharide (EPS) of the multiresistant clinical strain of Klebsiella pneumoniae, PCM2713, and thus should be regarded as a dual-function macromolecule that exhibits both structural and enzymatic actions. Here, we present our crystallographic and enzymatic studies of TTPA. TTPA was crystallized and X-ray diffraction data were collected to a resolution of 1.9 A. In the crystal, TTPA molecules were found to adopt a tetrameric structure with alpha-helical domains on one side and beta-strands and loops on the other. The novel crystal structure of TTPA resembles those of the bacteriophage T7 tail protein gp11 and gp4 of bacteriophage P22, but TTPA contains an additional antiparallel beta-sheet carrying a lectin-like domain that could be responsible for EPS binding. The enzymatic activity of TTPA may reflect the presence of a peptidoglycan hydrolase domain in the alpha-helical region (amino acid residues 126 to 173). These novel results provide new insights into the enzymatic mechanism through which TTPA acts on polysaccharides.

-	~~Authors~~: Pyra, A., Brzozowska, E., Pawlik, K., Dauter, M., Dauter, Z.~~, Gamian, A~~.	+	Tail tubular protein A: a dual-function tail protein of Klebsiella pneumoniae bacteriophage KP32.,Pyra A, Brzozowska E, Pawlik K, Gamian A, Dauter M, Dauter Z Sci Rep. 2017 May 22;7(1):2223. doi: 10.1038/s41598-017-02451-3. PMID:28533535<ref>PMID:28533535</ref>

-	~~Description: Tail Tubular Protein A~~ of ~~Klebsiella pneumoniae bacteriophage KP32~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Pawlik~~, K]]	+	<div class="pdbe-citations 5mu4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Brzozowska, E]]
		+	[[Category: Dauter, M]]
	[[Category: Dauter, Z]]		[[Category: Dauter, Z]]
	[[Category: Gamian, A]]		[[Category: Gamian, A]]
-	[[Category: ~~Brzozowska~~, E]]	+	[[Category: Pawlik, K]]
	[[Category: Pyra, A]]		[[Category: Pyra, A]]
-	[[Category: ~~Dauter, M~~]]	+	[[Category: Antibacterial activity]]
		+	[[Category: Bacteriophage]]
		+	[[Category: Exopolysaccharide depolymerase]]
		+	[[Category: Tail tubular protein]]
		+	[[Category: Viral protein]]

OCA at 11:39, 9 March 2017

OCA — Thu, 09 Mar 2017 11:39:21 GMT

←Older revision		Revision as of 11:39, 9 March 2017
Line 9:		Line 9:
	[[Category: Pawlik, K]]		[[Category: Pawlik, K]]
	[[Category: Dauter, Z]]		[[Category: Dauter, Z]]
-	[[Category: Brzozowska, E]]
-	[[Category: Dauter, M]]
	[[Category: Gamian, A]]		[[Category: Gamian, A]]
		+	[[Category: Brzozowska, E]]
	[[Category: Pyra, A]]		[[Category: Pyra, A]]
		+	[[Category: Dauter, M]]

OCA: Protected "5mu4" [edit=sysop:move=sysop]

OCA — Wed, 18 Jan 2017 13:16:48 GMT

Protected "5mu4" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:16, 18 January 2017

OCA: New page: '''Unreleased structure''' The entry 5mu4 is ON HOLD Authors: Pyra, A., Brzozowska, E., Pawlik, K., Dauter, M., Dauter, Z., Gamian, A. Description: Tail Tubular Protein A of Klebsiella...

OCA — Wed, 18 Jan 2017 13:16:46 GMT

New page: '''Unreleased structure''' The entry 5mu4 is ON HOLD Authors: Pyra, A., Brzozowska, E., Pawlik, K., Dauter, M., Dauter, Z., Gamian, A. Description: Tail Tubular Protein A of Klebsiella...

New page

'''Unreleased structure'''

The entry 5mu4 is ON HOLD

Authors: Pyra, A., Brzozowska, E., Pawlik, K., Dauter, M., Dauter, Z., Gamian, A.

Description: Tail Tubular Protein A of Klebsiella pneumoniae bacteriophage KP32
[[Category: Unreleased Structures]]
[[Category: Pawlik, K]]
[[Category: Dauter, Z]]
[[Category: Brzozowska, E]]
[[Category: Dauter, M]]
[[Category: Gamian, A]]
[[Category: Pyra, A]]