5mua - Revision history

OCA at 17:50, 8 November 2023

OCA — Wed, 08 Nov 2023 17:50:09 GMT

←Older revision		Revision as of 17:50, 8 November 2023
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	<StructureSection load='5mua' size='340' side='right'caption='[[5mua]], [[Resolution\|resolution]] 1.49Å' scene=''>		<StructureSection load='5mua' size='340' side='right'caption='[[5mua]], [[Resolution\|resolution]] 1.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mua]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Polyporus_squamosus Polyporus~~ squamosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5MUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mua]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cerioporus_squamosus Cerioporus squamosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MUA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.49Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3phz\|3phz]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">psl1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5640 Polyporus squamosus])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mua OCA], [https://pdbe.org/5mua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mua RCSB], [https://www.ebi.ac.uk/pdbsum/5mua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mua ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5mua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mua OCA], [~~http~~://pdbe.org/5mua PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mua RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mua PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mua ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q75WT9_9APHY Q75WT9_9APHY]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cerioporus squamosus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Polyporus squamosus]]~~	+	[[Category: Cordara G]]
-	[[Category: Cordara, G]]	+	[[Category: Krengel U]]
-	[[Category: Krengel, U]]	+	[[Category: Manna D]]
-	[[Category: Manna, D]]	+
-	~~[[Category: Calcium-binding]]~~	+
-	~~[[Category: E-64 inhibitor complex]]~~	+
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: Papain-like protease]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 07:29, 19 August 2020

OCA — Wed, 19 Aug 2020 07:29:15 GMT

←Older revision		Revision as of 07:29, 19 August 2020
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	==PSL1a-E64 complex==		==PSL1a-E64 complex==
-	<StructureSection load='5mua' size='340' side='right' caption='[[5mua]], [[Resolution\|resolution]] 1.49Å' scene=''>	+	<StructureSection load='5mua' size='340' side='right'caption='[[5mua]], [[Resolution\|resolution]] 1.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mua]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mua]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Polyporus_squamosus Polyporus squamosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MUA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3phz\|3phz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3phz\|3phz]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mua OCA], [http://pdbe.org/5mua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mua RCSB], [http://www.ebi.ac.uk/pdbsum/5mua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mua ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">psl1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5640 Polyporus squamosus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mua OCA], [http://pdbe.org/5mua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mua RCSB], [http://www.ebi.ac.uk/pdbsum/5mua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mua ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Polyporus squamosus]]
	[[Category: Cordara, G]]		[[Category: Cordara, G]]
	[[Category: Krengel, U]]		[[Category: Krengel, U]]

OCA at 03:56, 30 August 2017

OCA — Wed, 30 Aug 2017 03:56:51 GMT

←Older revision		Revision as of 03:56, 30 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mua is ~~ON HOLD~~	+	==PSL1a-E64 complex==
		+	<StructureSection load='5mua' size='340' side='right' caption='[[5mua]], [[Resolution\|resolution]] 1.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mua]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MUA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=E64:N-[N-[1-HYDROXYCARBOXYETHYL-CARBONYL]LEUCYLAMINO-BUTYL]-GUANIDINE'>E64</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3phz\|3phz]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mua OCA], [http://pdbe.org/5mua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mua RCSB], [http://www.ebi.ac.uk/pdbsum/5mua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mua ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	An important function of fungal lectins is to protect their host. Marasmius oreades agglutinin (MOA) is toxic to nematodes and exerts its protective effect through protease activity. Its proteolytic function is associated with a papain-like dimerization domain. The closest homologue of MOA is Polyporus squamosus lectin 1a (PSL1a). Here, we probed PSL1a for catalytic activity and confirmed that it is a calcium-dependent cysteine protease, like MOA. The X-ray crystal structures of PSL1a (1.5 A) and MOA (1.3 A) in complex with calcium and the irreversible cysteine protease inhibitor E-64 elucidated the structural basis for their mechanism of action. The comparison with other calcium-dependent proteases (calpains, LapG) reveals a unique metal-dependent activation mechanism relying on a calcium-induced backbone shift and intradimer cooperation. Intriguingly, the enzymes appear to use a tyrosine-gating mechanism instead of pro-peptide processing. A search for potential MOA orthologues suggests the existence of a whole new family of fungal chimerolectins with these unique features.

-	~~Authors: Cordara~~, G., Manna, D., Krengel, U.	+	Family of Papain-Like Fungal Chimerolectins with Distinct Ca2+-Dependent Activation Mechanism.,Cordara G, Manna D, Krengel U Biochemistry. 2017 Aug 22. doi: 10.1021/acs.biochem.7b00317. PMID:28665586<ref>PMID:28665586</ref>

-	~~Description: PSL1a~~-~~E64 complex~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5mua" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cordara, G]]
	[[Category: Krengel, U]]		[[Category: Krengel, U]]
	[[Category: Manna, D]]		[[Category: Manna, D]]
-	[[Category: ~~Cordara, G~~]]	+	[[Category: Calcium-binding]]
		+	[[Category: E-64 inhibitor complex]]
		+	[[Category: Lectin]]
		+	[[Category: Papain-like protease]]
		+	[[Category: Sugar binding protein]]

OCA: Protected "5mua" [edit=sysop:move=sysop]

OCA — Wed, 18 Jan 2017 13:17:32 GMT

Protected "5mua" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:17, 18 January 2017

OCA: New page: '''Unreleased structure''' The entry 5mua is ON HOLD Authors: Cordara, G., Manna, D., Krengel, U. Description: PSL1a-E64 complex Category: Unreleased Structures [[Category: Krengel...

OCA — Wed, 18 Jan 2017 13:17:31 GMT

New page: '''Unreleased structure''' The entry 5mua is ON HOLD Authors: Cordara, G., Manna, D., Krengel, U. Description: PSL1a-E64 complex Category: Unreleased Structures [[Category: Krengel...

New page

'''Unreleased structure'''

The entry 5mua is ON HOLD

Authors: Cordara, G., Manna, D., Krengel, U.

Description: PSL1a-E64 complex
[[Category: Unreleased Structures]]
[[Category: Krengel, U]]
[[Category: Manna, D]]
[[Category: Cordara, G]]