5mus - Revision history

OCA at 17:50, 8 November 2023

OCA — Wed, 08 Nov 2023 17:50:43 GMT

←Older revision		Revision as of 17:50, 8 November 2023
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	<StructureSection load='5mus' size='340' side='right'caption='[[5mus]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='5mus' size='340' side='right'caption='[[5mus]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mus]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cas_virus Cas~~ virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5MUS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mus]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/CAS_virus CAS virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MUS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.009Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5mus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mus OCA], [~~http~~://pdbe.org/5mus PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mus RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mus PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mus ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mus OCA], [https://pdbe.org/5mus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mus RCSB], [https://www.ebi.ac.uk/pdbsum/5mus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mus ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/J7HBG8_9VIRU J7HBG8_9VIRU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cas~~ virus]]	+	[[Category: CAS virus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cusack, S]]	+	[[Category: Cusack S]]
-	[[Category: Gogrefe, N]]	+	[[Category: Gogrefe N]]
-	[[Category: Gunther, S]]	+	[[Category: Gunther S]]
-	[[Category: Rauschenberger, B]]	+	[[Category: Rauschenberger B]]
-	[[Category: Reguera, J]]	+	[[Category: Reguera J]]
-	[[Category: Reindl, S]]	+	[[Category: Reindl S]]
-	[[Category: Rosenthal, M]]	+	[[Category: Rosenthal M]]
-	[[Category: Vogel, D]]	+	[[Category: Vogel D]]
-	~~[[Category: Arenavirus]]~~	+
-	~~[[Category: Polymerase]]~~	+
-	~~[[Category: Putative cap-binding]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 07:29, 19 August 2020

OCA — Wed, 19 Aug 2020 07:29:34 GMT

←Older revision		Revision as of 07:29, 19 August 2020
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	==Structure of the C-terminal domain of a reptarenavirus L protein==		==Structure of the C-terminal domain of a reptarenavirus L protein==
-	<StructureSection load='5mus' size='340' side='right' caption='[[5mus]], [[Resolution\|resolution]] 2.01Å' scene=''>	+	<StructureSection load='5mus' size='340' side='right'caption='[[5mus]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mus]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MUS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mus]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cas_virus Cas virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MUS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mus OCA], [http://pdbe.org/5mus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mus RCSB], [http://www.ebi.ac.uk/pdbsum/5mus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mus ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mus OCA], [http://pdbe.org/5mus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mus RCSB], [http://www.ebi.ac.uk/pdbsum/5mus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mus ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cas virus]]
		+	[[Category: Large Structures]]
	[[Category: Cusack, S]]		[[Category: Cusack, S]]
	[[Category: Gogrefe, N]]		[[Category: Gogrefe, N]]

OCA at 07:22, 24 May 2017

OCA — Wed, 24 May 2017 07:22:44 GMT

←Older revision		Revision as of 07:22, 24 May 2017
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mus OCA], [http://pdbe.org/5mus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mus RCSB], [http://www.ebi.ac.uk/pdbsum/5mus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mus ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mus OCA], [http://pdbe.org/5mus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mus RCSB], [http://www.ebi.ac.uk/pdbsum/5mus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mus ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cap-snatching was first discovered in influenza virus. Structures of the involved domains of the influenza virus polymerase, namely the endonuclease in the PA subunit and the cap-binding domain in the PB2 subunit, have been solved. Cap-snatching endonucleases have also been demonstrated at the very N-terminus of the L proteins of mammarena-, orthobunya-, and hantaviruses. However, a cap-binding domain has not been identified in an arena- or bunyavirus L protein so far. We solved the structure of the 326 C-terminal residues of the L protein of California Academy of Sciences virus (CASV), a reptarenavirus, by X-ray crystallography. The individual domains of this 37-kDa fragment (L-Cterm) as well as the domain arrangement are structurally similar to the cap-binding and adjacent domains of influenza virus polymerase PB2 subunit, despite the absence of sequence homology, suggesting a common evolutionary origin. This enabled identification of a region in CASV L-Cterm with similarity to a cap-binding site; however, the typical sandwich of two aromatic residues was missing. Consistent with this, cap-binding to CASV L-Cterm could not be detected biochemically. In addition, we solved the crystal structure of the corresponding endonuclease in the N-terminus of CASV L protein. It shows a typical endonuclease fold with an active site configuration that is essentially identical to that of known mammarenavirus endonuclease structures. In conclusion, we provide evidence for a presumably functional cap-snatching endonuclease in the N-terminus and a degenerate cap-binding domain in the C-terminus of a reptarenavirus L protein. Implications of these findings for the cap-snatching mechanism in arenaviruses are discussed.
		+
		+	Structural insights into reptarenavirus cap-snatching machinery.,Rosenthal M, Gogrefe N, Vogel D, Reguera J, Rauschenberger B, Cusack S, Gunther S, Reindl S PLoS Pathog. 2017 May 15;13(5):e1006400. doi: 10.1371/journal.ppat.1006400. PMID:28505175<ref>PMID:28505175</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5mus" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:48, 17 May 2017

OCA — Wed, 17 May 2017 15:48:22 GMT

←Older revision		Revision as of 15:48, 17 May 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mus is ~~ON HOLD until Paper Publication~~	+	==Structure of the C-terminal domain of a reptarenavirus L protein==
-		+	<StructureSection load='5mus' size='340' side='right' caption='[[5mus]], [[Resolution\|resolution]] 2.01Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5mus]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MUS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mus OCA], [http://pdbe.org/5mus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mus RCSB], [http://www.ebi.ac.uk/pdbsum/5mus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mus ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cusack, S]]
		+	[[Category: Gogrefe, N]]
		+	[[Category: Gunther, S]]
		+	[[Category: Rauschenberger, B]]
		+	[[Category: Reguera, J]]
		+	[[Category: Reindl, S]]
		+	[[Category: Rosenthal, M]]
		+	[[Category: Vogel, D]]
		+	[[Category: Arenavirus]]
		+	[[Category: Polymerase]]
		+	[[Category: Putative cap-binding]]
		+	[[Category: Viral protein]]

OCA: Protected "5mus" [edit=sysop:move=sysop]

OCA — Wed, 25 Jan 2017 20:35:50 GMT

Protected "5mus" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:35, 25 January 2017

OCA: New page: '''Unreleased structure''' The entry 5mus is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 Jan 2017 20:35:49 GMT

New page: '''Unreleased structure''' The entry 5mus is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5mus is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]