5mwo - Revision history

OCA at 17:52, 8 November 2023

OCA — Wed, 08 Nov 2023 17:52:23 GMT

←Older revision		Revision as of 17:52, 8 November 2023
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	<StructureSection load='5mwo' size='340' side='right'caption='[[5mwo]], [[Resolution\|resolution]] 1.96Å' scene=''>		<StructureSection load='5mwo' size='340' side='right'caption='[[5mwo]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mwo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MWO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5MWO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mwo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MWO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=J6W:(5-methyl-1-benzothiophen-2-yl)methanol'>J6W</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.962Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mwo OCA], [~~http~~://pdbe.org/5mwo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mwo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mwo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mwo ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=J6W:(5-methyl-1-benzothiophen-2-yl)methanol'>J6W</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mwo OCA], [https://pdbe.org/5mwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mwo RCSB], [https://www.ebi.ac.uk/pdbsum/5mwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mwo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ETHR_MYCTU ETHR_MYCTU]] Involved in the repression of the monooxygenase EthA which is responsible of the formation of the active metabolite of ethionamide (ETH).<ref>PMID:10869356</ref> <ref>PMID:10944230</ref>	+	[https://www.uniprot.org/uniprot/ETHR_MYCTU ETHR_MYCTU] Involved in the repression of the monooxygenase EthA which is responsible of the formation of the active metabolite of ethionamide (ETH).<ref>PMID:10869356</ref> <ref>PMID:10944230</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5mwo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5mwo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abell, C]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Blundell~~, T L~~]]	+	[[Category: Abell C]]
-	[[Category: Chan~~, D S.~~H]]	+	[[Category: Blundell TL]]
-	[[Category: Coyne~~, A G~~]]	+	[[Category: Chan DS-H]]
-	[[Category: Matak-Vinkovic, D]]	+	[[Category: Coyne AG]]
-	[[Category: McConnell, B]]	+	[[Category: Matak-Vinkovic D]]
-	[[Category: Mendes, V]]	+	[[Category: McConnell B]]
-	[[Category: Thomas~~, S E]]~~	+	[[Category: Mendes V]]
-	~~[[Category: Ethr]]~~	+	[[Category: Thomas SE]]
-	~~[[Category: Mycobacterium tuberculosis]]~~	+
-	~~[[Category: Repressor]]~~	+
-	~~[[Category: Tetr]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 07:31, 19 August 2020

OCA — Wed, 19 Aug 2020 07:31:00 GMT

←Older revision		Revision as of 07:31, 19 August 2020
Line 1:		Line 1:

	==Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7E8.==		==Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7E8.==
-	<StructureSection load='5mwo' size='340' side='right' caption='[[5mwo]], [[Resolution\|resolution]] 1.96Å' scene=''>	+	<StructureSection load='5mwo' size='340' side='right'caption='[[5mwo]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mwo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MWO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MWO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mwo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MWO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MWO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=J6W:(5-methyl-1-benzothiophen-2-yl)methanol'>J6W</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=J6W:(5-methyl-1-benzothiophen-2-yl)methanol'>J6W</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mwo OCA], [http://pdbe.org/5mwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mwo RCSB], [http://www.ebi.ac.uk/pdbsum/5mwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mwo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mwo OCA], [http://pdbe.org/5mwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mwo RCSB], [http://www.ebi.ac.uk/pdbsum/5mwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mwo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Abell, C]]		[[Category: Abell, C]]
	[[Category: Blundell, T L]]		[[Category: Blundell, T L]]

OCA at 08:54, 3 August 2017

OCA — Thu, 03 Aug 2017 08:54:21 GMT

←Older revision		Revision as of 08:54, 3 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mwo is ~~ON HOLD~~ ~~until Jan 18 2019~~	+	==Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7E8.==
		+	<StructureSection load='5mwo' size='340' side='right' caption='[[5mwo]], [[Resolution\|resolution]] 1.96Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mwo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MWO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=J6W:(5-methyl-1-benzothiophen-2-yl)methanol'>J6W</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mwo OCA], [http://pdbe.org/5mwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mwo RCSB], [http://www.ebi.ac.uk/pdbsum/5mwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mwo ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ETHR_MYCTU ETHR_MYCTU]] Involved in the repression of the monooxygenase EthA which is responsible of the formation of the active metabolite of ethionamide (ETH).<ref>PMID:10869356</ref> <ref>PMID:10944230</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Native nanoelectrospray ionization mass spectrometry is an underutilized technique for fragment screening. In this study, the first demonstration is provided of the use of native mass spectrometry for screening fragments against a protein-DNA interaction. EthR is a transcriptional repressor of EthA expression in Mycobacterium tuberculosis (Mtb) that reduces the efficacy of ethionamide, a second-line antitubercular drug used to combat multidrug-resistant Mtb strains. A small-scale fragment screening campaign was conducted against the EthR-DNA interaction using native mass spectrometry, and the results were compared with those from differential scanning fluorimetry, a commonly used primary screening technique. Hits were validated by surface plasmon resonance and X-ray crystallography. The screening campaign identified two new fragments that disrupt the EthR-DNA interaction in vitro (IC50 =460-610 mum) and bind to the hydrophobic channel of the EthR dimer.

-	~~Authors: Mendes, V~~., Chan, ~~D.S.-H.~~, Thomas~~, S.E.~~, McConnell~~, B.~~, Matak-Vinkovic, D., Coyne, ~~A.G.~~, Abell, C., ~~Blundell, T~~.L.	+	Fragment Screening against the EthR-DNA Interaction by Native Mass Spectrometry.,Chan DS, Mendes V, Thomas SE, McConnell BN, Matak-Vinkovic D, Coyne AG, Blundell TL, Abell C Angew Chem Int Ed Engl. 2017 Jun 19;56(26):7488-7491. doi:, 10.1002/anie.201702888. Epub 2017 May 31. PMID:28513917<ref>PMID:28513917</ref>

-	~~Description: Structure~~ of ~~Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7E8~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Blundell, T.L]]~~	+	<div class="pdbe-citations 5mwo" style="background-color:#fffaf0;"></div>
-	~~[[Category: Mendes, V]]~~	+	== References ==
-	~~[[Category: Thomas, S.E]]~~	+	<references/>
-	~~[[Category: Matak-Vinkovic, D]]~~	+	__TOC__
		+	</StructureSection>
	[[Category: Abell, C]]		[[Category: Abell, C]]
-	[[Category: ~~Mcconnell~~, B]]	+	[[Category: Blundell, T L]]
-	[[Category: Coyne, A.G]]	+	[[Category: Chan, D S.H]]
-	[[Category: ~~Chan~~, D.S~~.-H~~]]	+	[[Category: Coyne, A G]]
		+	[[Category: Matak-Vinkovic, D]]
		+	[[Category: McConnell, B]]
		+	[[Category: Mendes, V]]
		+	[[Category: Thomas, S E]]
		+	[[Category: Ethr]]
		+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Repressor]]
		+	[[Category: Tetr]]
		+	[[Category: Transcription]]

OCA at 11:43, 9 March 2017

OCA — Thu, 09 Mar 2017 11:43:53 GMT

←Older revision		Revision as of 11:43, 9 March 2017
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	Description: Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7E8.		Description: Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7E8.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Blundell, T.L]]
		+	[[Category: Mendes, V]]
	[[Category: Thomas, S.E]]		[[Category: Thomas, S.E]]
	[[Category: Matak-Vinkovic, D]]		[[Category: Matak-Vinkovic, D]]
	[[Category: Abell, C]]		[[Category: Abell, C]]
-	[[Category: Coyne, A.G]]
-	[[Category: Blundell, T.L]]
-	[[Category: Mendes, V]]
	[[Category: Mcconnell, B]]		[[Category: Mcconnell, B]]
		+	[[Category: Coyne, A.G]]
	[[Category: Chan, D.S.-H]]		[[Category: Chan, D.S.-H]]

OCA at 15:18, 1 February 2017

OCA — Wed, 01 Feb 2017 15:18:22 GMT

←Older revision		Revision as of 15:18, 1 February 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5mwo is ON HOLD	+	The entry 5mwo is ON HOLD until Jan 18 2019

	Authors: Mendes, V., Chan, D.S.-H., Thomas, S.E., McConnell, B., Matak-Vinkovic, D., Coyne, A.G., Abell, C., Blundell, T.L.		Authors: Mendes, V., Chan, D.S.-H., Thomas, S.E., McConnell, B., Matak-Vinkovic, D., Coyne, A.G., Abell, C., Blundell, T.L.

OCA: Protected "5mwo" [edit=sysop:move=sysop]

OCA — Wed, 25 Jan 2017 20:41:12 GMT

Protected "5mwo" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:41, 25 January 2017

OCA: New page: '''Unreleased structure''' The entry 5mwo is ON HOLD Authors: Mendes, V., Chan, D.S.-H., Thomas, S.E., McConnell, B., Matak-Vinkovic, D., Coyne, A.G., Abell, C., Blundell, T.L. Descrip...

OCA — Wed, 25 Jan 2017 20:41:10 GMT

New page: '''Unreleased structure''' The entry 5mwo is ON HOLD Authors: Mendes, V., Chan, D.S.-H., Thomas, S.E., McConnell, B., Matak-Vinkovic, D., Coyne, A.G., Abell, C., Blundell, T.L. Descrip...

New page

'''Unreleased structure'''

The entry 5mwo is ON HOLD

Authors: Mendes, V., Chan, D.S.-H., Thomas, S.E., McConnell, B., Matak-Vinkovic, D., Coyne, A.G., Abell, C., Blundell, T.L.

Description: Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7E8.
[[Category: Unreleased Structures]]
[[Category: Thomas, S.E]]
[[Category: Matak-Vinkovic, D]]
[[Category: Abell, C]]
[[Category: Coyne, A.G]]
[[Category: Blundell, T.L]]
[[Category: Mendes, V]]
[[Category: Mcconnell, B]]
[[Category: Chan, D.S.-H]]