5mxk - Revision history

OCA at 17:53, 8 November 2023

OCA — Wed, 08 Nov 2023 17:53:06 GMT

←Older revision		Revision as of 17:53, 8 November 2023
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	<StructureSection load='5mxk' size='340' side='right'caption='[[5mxk]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='5mxk' size='340' side='right'caption='[[5mxk]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mxk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MXK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5MXK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mxk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MXK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MXK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZHA:~{N}-(5-oxidanylidene-7,8-dihydro-6~{H}-naphthalen-2-yl)ethanamide'>ZHA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.932Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5mxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mxk OCA], [~~http~~://pdbe.org/5mxk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mxk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mxk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mxk ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZHA:~{N}-(5-oxidanylidene-7,8-dihydro-6~{H}-naphthalen-2-yl)ethanamide'>ZHA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mxk OCA], [https://pdbe.org/5mxk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mxk RCSB], [https://www.ebi.ac.uk/pdbsum/5mxk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mxk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ETHR_MYCTU ETHR_MYCTU]] Involved in the repression of the monooxygenase EthA which is responsible of the formation of the active metabolite of ethionamide (ETH).<ref>PMID:10869356</ref> <ref>PMID:10944230</ref>	+	[https://www.uniprot.org/uniprot/ETHR_MYCTU ETHR_MYCTU] Involved in the repression of the monooxygenase EthA which is responsible of the formation of the active metabolite of ethionamide (ETH).<ref>PMID:10869356</ref> <ref>PMID:10944230</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5mxk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5mxk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abell, C]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Blundell~~, T L~~]]	+	[[Category: Abell C]]
-	[[Category: Chan~~, D S.~~H]]	+	[[Category: Blundell TL]]
-	[[Category: Coyne~~, A G~~]]	+	[[Category: Chan DS-H]]
-	[[Category: Matak-Vinkovic, D]]	+	[[Category: Coyne AG]]
-	[[Category: McConnell, B]]	+	[[Category: Matak-Vinkovic D]]
-	[[Category: Mendes, V]]	+	[[Category: McConnell B]]
-	[[Category: Thomas~~, S E]]~~	+	[[Category: Mendes V]]
-	~~[[Category: Ethr]]~~	+	[[Category: Thomas SE]]
-	~~[[Category: Mycobacterium tuberculosis]]~~	+
-	~~[[Category: Repressor]]~~	+
-	~~[[Category: Tetr]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 07:31, 19 August 2020

OCA — Wed, 19 Aug 2020 07:31:33 GMT

←Older revision		Revision as of 07:31, 19 August 2020
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	==Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7G9.==		==Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7G9.==
-	<StructureSection load='5mxk' size='340' side='right' caption='[[5mxk]], [[Resolution\|resolution]] 1.93Å' scene=''>	+	<StructureSection load='5mxk' size='340' side='right'caption='[[5mxk]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mxk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MXK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MXK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mxk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MXK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MXK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZHA:~{N}-(5-oxidanylidene-7,8-dihydro-6~{H}-naphthalen-2-yl)ethanamide'>ZHA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZHA:~{N}-(5-oxidanylidene-7,8-dihydro-6~{H}-naphthalen-2-yl)ethanamide'>ZHA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mxk OCA], [http://pdbe.org/5mxk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mxk RCSB], [http://www.ebi.ac.uk/pdbsum/5mxk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mxk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mxk OCA], [http://pdbe.org/5mxk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mxk RCSB], [http://www.ebi.ac.uk/pdbsum/5mxk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mxk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Abell, C]]		[[Category: Abell, C]]
	[[Category: Blundell, T L]]		[[Category: Blundell, T L]]

OCA at 08:18, 3 August 2017

OCA — Thu, 03 Aug 2017 08:18:21 GMT

←Older revision		Revision as of 08:18, 3 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mxk is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7G9.==
		+	<StructureSection load='5mxk' size='340' side='right' caption='[[5mxk]], [[Resolution\|resolution]] 1.93Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mxk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MXK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MXK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZHA:~{N}-(5-oxidanylidene-7,8-dihydro-6~{H}-naphthalen-2-yl)ethanamide'>ZHA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mxk OCA], [http://pdbe.org/5mxk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mxk RCSB], [http://www.ebi.ac.uk/pdbsum/5mxk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mxk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ETHR_MYCTU ETHR_MYCTU]] Involved in the repression of the monooxygenase EthA which is responsible of the formation of the active metabolite of ethionamide (ETH).<ref>PMID:10869356</ref> <ref>PMID:10944230</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Native nanoelectrospray ionization mass spectrometry is an underutilized technique for fragment screening. In this study, the first demonstration is provided of the use of native mass spectrometry for screening fragments against a protein-DNA interaction. EthR is a transcriptional repressor of EthA expression in Mycobacterium tuberculosis (Mtb) that reduces the efficacy of ethionamide, a second-line antitubercular drug used to combat multidrug-resistant Mtb strains. A small-scale fragment screening campaign was conducted against the EthR-DNA interaction using native mass spectrometry, and the results were compared with those from differential scanning fluorimetry, a commonly used primary screening technique. Hits were validated by surface plasmon resonance and X-ray crystallography. The screening campaign identified two new fragments that disrupt the EthR-DNA interaction in vitro (IC50 =460-610 mum) and bind to the hydrophobic channel of the EthR dimer.

-	~~Authors: Mendes, V~~., Chan, ~~D.S.-H.~~, Thomas~~, S.E.~~, McConnell~~, B.~~, Matak-Vinkovic, D., Coyne, ~~A.G.~~, Abell, C., ~~Blundell, T~~.L.	+	Fragment Screening against the EthR-DNA Interaction by Native Mass Spectrometry.,Chan DS, Mendes V, Thomas SE, McConnell BN, Matak-Vinkovic D, Coyne AG, Blundell TL, Abell C Angew Chem Int Ed Engl. 2017 Jun 19;56(26):7488-7491. doi:, 10.1002/anie.201702888. Epub 2017 May 31. PMID:28513917<ref>PMID:28513917</ref>

-	~~Description: Structure~~ of ~~Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7G9~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Blundell, T.L]]~~	+	<div class="pdbe-citations 5mxk" style="background-color:#fffaf0;"></div>
-	~~[[Category: Mendes, V]]~~	+	== References ==
-	~~[[Category: Thomas, S.E]]~~	+	<references/>
-	~~[[Category: Matak-Vinkovic, D]]~~	+	__TOC__
		+	</StructureSection>
	[[Category: Abell, C]]		[[Category: Abell, C]]
-	[[Category: ~~Mcconnell~~, B]]	+	[[Category: Blundell, T L]]
-	[[Category: Coyne, A.G]]	+	[[Category: Chan, D S.H]]
-	[[Category: ~~Chan~~, D.S~~.-H~~]]	+	[[Category: Coyne, A G]]
		+	[[Category: Matak-Vinkovic, D]]
		+	[[Category: McConnell, B]]
		+	[[Category: Mendes, V]]
		+	[[Category: Thomas, S E]]
		+	[[Category: Ethr]]
		+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Repressor]]
		+	[[Category: Tetr]]
		+	[[Category: Transcription]]

OCA at 11:45, 9 March 2017

OCA — Thu, 09 Mar 2017 11:45:33 GMT

←Older revision		Revision as of 11:45, 9 March 2017
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	Description: Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7G9.		Description: Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7G9.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Blundell, T.L]]
		+	[[Category: Mendes, V]]
	[[Category: Thomas, S.E]]		[[Category: Thomas, S.E]]
	[[Category: Matak-Vinkovic, D]]		[[Category: Matak-Vinkovic, D]]
	[[Category: Abell, C]]		[[Category: Abell, C]]
-	[[Category: Coyne, A.G]]
-	[[Category: Blundell, T.L]]
-	[[Category: Mendes, V]]
	[[Category: Mcconnell, B]]		[[Category: Mcconnell, B]]
		+	[[Category: Coyne, A.G]]
	[[Category: Chan, D.S.-H]]		[[Category: Chan, D.S.-H]]

OCA: Protected "5mxk" [edit=sysop:move=sysop]

OCA — Wed, 01 Feb 2017 15:21:35 GMT

Protected "5mxk" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:21, 1 February 2017

OCA: New page: '''Unreleased structure''' The entry 5mxk is ON HOLD until Paper Publication Authors: Mendes, V., Chan, D.S.-H., Thomas, S.E., McConnell, B., Matak-Vinkovic, D., Coyne, A.G., Abell, C.,...

OCA — Wed, 01 Feb 2017 15:21:33 GMT

New page: '''Unreleased structure''' The entry 5mxk is ON HOLD until Paper Publication Authors: Mendes, V., Chan, D.S.-H., Thomas, S.E., McConnell, B., Matak-Vinkovic, D., Coyne, A.G., Abell, C.,...

New page

'''Unreleased structure'''

The entry 5mxk is ON HOLD until Paper Publication

Authors: Mendes, V., Chan, D.S.-H., Thomas, S.E., McConnell, B., Matak-Vinkovic, D., Coyne, A.G., Abell, C., Blundell, T.L.

Description: Structure of Mycobacterium Tuberculosis Transcriptional Regulatory Repressor Protein (EthR) in complex with fragment 7G9.
[[Category: Unreleased Structures]]
[[Category: Thomas, S.E]]
[[Category: Matak-Vinkovic, D]]
[[Category: Abell, C]]
[[Category: Coyne, A.G]]
[[Category: Blundell, T.L]]
[[Category: Mendes, V]]
[[Category: Mcconnell, B]]
[[Category: Chan, D.S.-H]]