5mye - Revision history

OCA at 17:53, 8 November 2023

OCA — Wed, 08 Nov 2023 17:53:59 GMT

←Older revision		Revision as of 17:53, 8 November 2023
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	==Solution structure of C20S variant of Dehydroascorbate reductase 3A from Populus trichocarpa in complex with dehydroascorbic acid.==		==Solution structure of C20S variant of Dehydroascorbate reductase 3A from Populus trichocarpa in complex with dehydroascorbic acid.==
-	<StructureSection load='5mye' size='340' side='right'caption='[[5mye~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='5mye' size='340' side='right'caption='[[5mye]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mye]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Poptr Poptr~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MYE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MYE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mye]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_trichocarpa Populus trichocarpa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MYE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MYE FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=UU3:(5R)-5-[(1S)-1,2-BIS(OXIDANYL)ETHYL]OXOLANE-2,3,4-TRIONE'>UU3</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~POPTR_0008s04920g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=3694 POPTR]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UU3:(5R)-5-[(1S)-1,2-BIS(OXIDANYL)ETHYL]OXOLANE-2,3,4-TRIONE'>UU3</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mye OCA], [~~http~~://pdbe.org/5mye PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mye RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mye PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mye ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mye OCA], [https://pdbe.org/5mye PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mye RCSB], [https://www.ebi.ac.uk/pdbsum/5mye PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mye ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B9HM36_POPTR B9HM36_POPTR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Poptr~~]]	+	[[Category: Populus trichocarpa]]
-	[[Category: Roret, T]]	+	[[Category: Roret T]]
-	[[Category: Tsan, P]]	+	[[Category: Tsan P]]
-	~~[[Category: Glutathione transferase dehydroascorbate reductase plant]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 13:12, 10 May 2019

OCA — Fri, 10 May 2019 13:12:11 GMT

←Older revision		Revision as of 13:12, 10 May 2019
Line 1:		Line 1:

	==Solution structure of C20S variant of Dehydroascorbate reductase 3A from Populus trichocarpa in complex with dehydroascorbic acid.==		==Solution structure of C20S variant of Dehydroascorbate reductase 3A from Populus trichocarpa in complex with dehydroascorbic acid.==
-	<StructureSection load='5mye' size='340' side='right' caption='[[5mye]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='5mye' size='340' side='right'caption='[[5mye]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mye]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MYE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MYE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mye]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Poptr Poptr]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MYE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MYE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UU3:(5R)-5-[(1S)-1,2-BIS(OXIDANYL)ETHYL]OXOLANE-2,3,4-TRIONE'>UU3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UU3:(5R)-5-[(1S)-1,2-BIS(OXIDANYL)ETHYL]OXOLANE-2,3,4-TRIONE'>UU3</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POPTR_0008s04920g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3694 POPTR])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mye OCA], [http://pdbe.org/5mye PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mye RCSB], [http://www.ebi.ac.uk/pdbsum/5mye PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mye ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mye OCA], [http://pdbe.org/5mye PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mye RCSB], [http://www.ebi.ac.uk/pdbsum/5mye PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mye ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Poptr]]
	[[Category: Roret, T]]		[[Category: Roret, T]]
	[[Category: Tsan, P]]		[[Category: Tsan, P]]
	[[Category: Glutathione transferase dehydroascorbate reductase plant]]		[[Category: Glutathione transferase dehydroascorbate reductase plant]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 16:17, 22 March 2017

OCA — Wed, 22 Mar 2017 16:17:32 GMT

←Older revision		Revision as of 16:17, 22 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mye is ~~ON HOLD~~	+	==Solution structure of C20S variant of Dehydroascorbate reductase 3A from Populus trichocarpa in complex with dehydroascorbic acid.==
		+	<StructureSection load='5mye' size='340' side='right' caption='[[5mye]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mye]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MYE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MYE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UU3:(5R)-5-[(1S)-1,2-BIS(OXIDANYL)ETHYL]OXOLANE-2,3,4-TRIONE'>UU3</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mye OCA], [http://pdbe.org/5mye PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mye RCSB], [http://www.ebi.ac.uk/pdbsum/5mye PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mye ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dehydroascorbate reductases (DHARs), enzymes belonging to the GST superfamily, catalyse the GSH-dependent reduction of dehydroascorbate into ascorbate in plants. By maintaining a reduced ascorbate pool, they notably participate to H2O2 detoxification catalysed by ascorbate peroxidases (APXs). Despite this central role, the catalytic mechanism used by DHARs is still not well understood and there is no supportive 3D structure. In this context, we have performed a thorough biochemical and structural analysis of the three poplar DHARs and coupled this to the analysis of their transcript expression patterns and subcellular localizations. The transcripts for these genes are mainly detected in reproductive and green organs and the corresponding proteins are expressed in plastids, in the cytosol and in the nucleus, but not in mitochondria and peroxisomes where ascorbate regeneration is obviously necessary. Comparing the kinetic properties and the sensitivity to GSSG-mediated oxidation of DHAR2 and DHAR3A, exhibiting 1 or 3 cysteinyl residues respectively, we observed that the presence of additional cysteines in DHAR3A modifies the regeneration mechanism of the catalytic cysteine by forming different redox states. Finally, from the 3D structure of DHAR3A solved by NMR, we were able to map the residues important for the binding of both substrates (GSH and DHA), showing that DHAR active site is very selective for DHA recognition and providing further insights into the catalytic mechanism and the roles of the additional cysteines found in some DHARs.

-	~~Authors~~: ~~Roret~~, T., Tsan, P.	+	Insights into ascorbate regeneration in plants: investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpa.,Lallement PA, Roret T, Tsan P, Gualberto JM, Girardet JM, Didierjean C, Rouhier N, Hecker A Biochem J. 2016 Mar 15;473(6):717-31. doi: 10.1042/BJ20151147. Epub 2015 Dec 23. PMID:26699905<ref>PMID:26699905</ref>

-	~~Description: Solution structure~~ of ~~C20S variant~~ of ~~Dehydroascorbate reductase 3A from Populus trichocarpa in complex with dehydroascorbic acid~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5mye" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Roret, T]]		[[Category: Roret, T]]
	[[Category: Tsan, P]]		[[Category: Tsan, P]]
		+	[[Category: Glutathione transferase dehydroascorbate reductase plant]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "5mye" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 11:47:31 GMT

Protected "5mye" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:47, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5mye is ON HOLD Authors: Roret, T., Tsan, P. Description: Solution structure of C20S variant of Dehydroascorbate reductase 3A from Populus trichoca...

OCA — Thu, 09 Mar 2017 11:47:29 GMT

New page: '''Unreleased structure''' The entry 5mye is ON HOLD Authors: Roret, T., Tsan, P. Description: Solution structure of C20S variant of Dehydroascorbate reductase 3A from Populus trichoca...

New page

'''Unreleased structure'''

The entry 5mye is ON HOLD

Authors: Roret, T., Tsan, P.

Description: Solution structure of C20S variant of Dehydroascorbate reductase 3A from Populus trichocarpa in complex with dehydroascorbic acid.
[[Category: Unreleased Structures]]
[[Category: Roret, T]]
[[Category: Tsan, P]]