5n0a - Revision history

OCA at 17:55, 8 November 2023

OCA — Wed, 08 Nov 2023 17:55:36 GMT

←Older revision		Revision as of 17:55, 8 November 2023
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	<StructureSection load='5n0a' size='340' side='right'caption='[[5n0a]], [[Resolution\|resolution]] 3.90Å' scene=''>		<StructureSection load='5n0a' size='340' side='right'caption='[[5n0a]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n0a]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5N0A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n0a]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N0A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.9Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5n0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0a OCA], [~~http~~://pdbe.org/5n0a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5n0a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5n0a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0a ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0a OCA], [https://pdbe.org/5n0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n0a RCSB], [https://www.ebi.ac.uk/pdbsum/5n0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0a ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0B4SHY9_9FLAV A0A0B4SHY9_9FLAV]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Dengue virus 2]]		[[Category: Dengue virus 2]]
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Guardado-Calvo, P]]	+	[[Category: Guardado-Calvo P]]
-	[[Category: Rey~~, F A~~]]	+	[[Category: Rey FA]]
-	[[Category: Rouvinski, A]]	+	[[Category: Rouvinski A]]
-	[[Category: Sharma, A]]	+	[[Category: Sharma A]]
-	[[Category: Vaney~~, M C]]~~	+	[[Category: Vaney MC]]
-	~~[[Category: Broadly neutralizing antibody]]~~	+
-	~~[[Category: Dengue virus]]~~	+
-	~~[[Category: Envelope fusion protein]]~~	+
-	~~[[Category: Immune system-viral protein complex~~]]	+

OCA at 07:34, 19 August 2020

OCA — Wed, 19 Aug 2020 07:34:11 GMT

←Older revision		Revision as of 07:34, 19 August 2020
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	==Crystal structure of A259C covalently linked dengue 2 virus envelope glycoprotein dimer in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 A11==		==Crystal structure of A259C covalently linked dengue 2 virus envelope glycoprotein dimer in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 A11==
-	<StructureSection load='5n0a' size='340' side='right' caption='[[5n0a]], [[Resolution\|resolution]] 3.90Å' scene=''>	+	<StructureSection load='5n0a' size='340' side='right'caption='[[5n0a]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n0a]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0A OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5N0A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n0a]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N0A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5n0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0a OCA], [http://pdbe.org/5n0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n0a RCSB], [http://www.ebi.ac.uk/pdbsum/5n0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0a OCA], [http://pdbe.org/5n0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n0a RCSB], [http://www.ebi.ac.uk/pdbsum/5n0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0a ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	[[Category: Dengue virus 2]]		[[Category: Dengue virus 2]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Guardado-Calvo, P]]		[[Category: Guardado-Calvo, P]]
	[[Category: Rey, F A]]		[[Category: Rey, F A]]

OCA at 10:49, 16 November 2017

OCA — Thu, 16 Nov 2017 10:49:21 GMT

←Older revision		Revision as of 10:49, 16 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5n0a is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of A259C covalently linked dengue 2 virus envelope glycoprotein dimer in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 A11==
		+	<StructureSection load='5n0a' size='340' side='right' caption='[[5n0a]], [[Resolution\|resolution]] 3.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5n0a]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N0A FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0a OCA], [http://pdbe.org/5n0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n0a RCSB], [http://www.ebi.ac.uk/pdbsum/5n0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0a ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A problem in the search for an efficient vaccine against dengue virus is the immunodominance of the fusion loop epitope (FLE), a segment of the envelope protein E that is buried at the interface of the E dimers coating mature viral particles. Anti-FLE antibodies are broadly cross-reactive but poorly neutralizing, displaying a strong infection enhancing potential. FLE exposure takes place via dynamic 'breathing' of E dimers at the virion surface. In contrast, antibodies targeting the E dimer epitope (EDE), readily exposed at the E dimer interface over the region of the conserved fusion loop, are very potent and broadly neutralizing. We here engineer E dimers locked by inter-subunit disulfide bonds, and show by X-ray crystallography and by binding to a panel of human antibodies that these engineered dimers do not expose the FLE, while retaining the EDE exposure. These locked dimers are strong immunogen candidates for a next-generation vaccine.

-	~~Authors~~:	+	Covalently linked dengue virus envelope glycoprotein dimers reduce exposure of the immunodominant fusion loop epitope.,Rouvinski A, Dejnirattisai W, Guardado-Calvo P, Vaney MC, Sharma A, Duquerroy S, Supasa P, Wongwiwat W, Haouz A, Barba-Spaeth G, Mongkolsapaya J, Rey FA, Screaton GR Nat Commun. 2017 May 23;8:15411. doi: 10.1038/ncomms15411. PMID:28534525<ref>PMID:28534525</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5n0a" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Dengue virus 2]]
		+	[[Category: Human]]
		+	[[Category: Guardado-Calvo, P]]
		+	[[Category: Rey, F A]]
		+	[[Category: Rouvinski, A]]
		+	[[Category: Sharma, A]]
		+	[[Category: Vaney, M C]]
		+	[[Category: Broadly neutralizing antibody]]
		+	[[Category: Dengue virus]]
		+	[[Category: Envelope fusion protein]]
		+	[[Category: Immune system-viral protein complex]]

OCA: Protected "5n0a" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 11:51:51 GMT

Protected "5n0a" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:51, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5n0a is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Thu, 09 Mar 2017 11:51:49 GMT

New page: '''Unreleased structure''' The entry 5n0a is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5n0a is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]