5n0w - Revision history

OCA at 17:56, 8 November 2023

OCA — Wed, 08 Nov 2023 17:56:09 GMT

←Older revision		Revision as of 17:56, 8 November 2023
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	<StructureSection load='5n0w' size='340' side='right'caption='[[5n0w]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='5n0w' size='340' side='right'caption='[[5n0w]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n0w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Jack_o'lantern Jack o'lantern~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5N0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n0w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Omphalotus_olearius Omphalotus olearius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N0W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5n0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0w OCA], [~~http~~://pdbe.org/5n0w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5n0w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5n0w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0w ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0w OCA], [https://pdbe.org/5n0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n0w RCSB], [https://www.ebi.ac.uk/pdbsum/5n0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2R2JFI2_OMPOL A0A2R2JFI2_OMPOL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Jack o'lantern]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Naismith, J H~~]]	+	[[Category: Omphalotus olearius]]
-	[[Category: ~~Song, H~~]]	+	[[Category: Naismith JH]]
-	[[Category: ~~Methyltransferase]]~~	+	[[Category: Song H]]
-	~~[[Category: Transferase~~]]	+

OCA at 11:55, 26 August 2020

OCA — Wed, 26 Aug 2020 11:55:06 GMT

←Older revision		Revision as of 11:55, 26 August 2020
Line 1:		Line 1:

	==Crystal structure of OphA-DeltaC6 mutant R72A in complex with SAM==		==Crystal structure of OphA-DeltaC6 mutant R72A in complex with SAM==
-	<StructureSection load='5n0w' size='340' side='right' caption='[[5n0w]], [[Resolution\|resolution]] 1.93Å' scene=''>	+	<StructureSection load='5n0w' size='340' side='right'caption='[[5n0w]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n0w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Jack_o'lantern Jack o'lantern]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0W OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5N0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n0w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Jack_o'lantern Jack o'lantern]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N0W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5n0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0w OCA], [http://pdbe.org/5n0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n0w RCSB], [http://www.ebi.ac.uk/pdbsum/5n0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0w OCA], [http://pdbe.org/5n0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n0w RCSB], [http://www.ebi.ac.uk/pdbsum/5n0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0w ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Jack o'lantern]]		[[Category: Jack o'lantern]]
		+	[[Category: Large Structures]]
	[[Category: Naismith, J H]]		[[Category: Naismith, J H]]
	[[Category: Song, H]]		[[Category: Song, H]]
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 20:06, 19 September 2018

OCA — Wed, 19 Sep 2018 20:06:09 GMT

←Older revision		Revision as of 20:06, 19 September 2018
Line 3:		Line 3:
	<StructureSection load='5n0w' size='340' side='right' caption='[[5n0w]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='5n0w' size='340' side='right' caption='[[5n0w]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n0w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n0w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Jack_o'lantern Jack o'lantern]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N0W FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0w OCA], [http://pdbe.org/5n0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n0w RCSB], [http://www.ebi.ac.uk/pdbsum/5n0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0w OCA], [http://pdbe.org/5n0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n0w RCSB], [http://www.ebi.ac.uk/pdbsum/5n0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0w ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The peptide bond, the defining feature of proteins, governs peptide chemistry by abolishing nucleophilicity of the nitrogen. This and the planarity of the peptide bond arise from the delocalization of the lone pair of electrons on the nitrogen atom into the adjacent carbonyl. While chemical methylation of an amide bond uses a strong base to generate the imidate, OphA, the precursor protein of the fungal peptide macrocycle omphalotin A, self-hypermethylates amides at pH 7 using S-adenosyl methionine (SAM) as cofactor. The structure of OphA reveals a complex catenane-like arrangement in which the peptide substrate is clamped with its amide nitrogen aligned for nucleophilic attack on the methyl group of SAM. Biochemical data and computational modeling suggest a base-catalyzed reaction with the protein stabilizing the reaction intermediate. Backbone N-methylation of peptides enhances their protease resistance and membrane permeability, a property that holds promise for applications to medicinal chemistry.
		+
		+	A molecular mechanism for the enzymatic methylation of nitrogen atoms within peptide bonds.,Song H, van der Velden NS, Shiran SL, Bleiziffer P, Zach C, Sieber R, Imani AS, Krausbeck F, Aebi M, Freeman MF, Riniker S, Kunzler M, Naismith JH Sci Adv. 2018 Aug 24;4(8):eaat2720. doi: 10.1126/sciadv.aat2720. eCollection 2018, Aug. PMID:30151425<ref>PMID:30151425</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5n0w" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Jack o'lantern]]
	[[Category: Naismith, J H]]		[[Category: Naismith, J H]]
	[[Category: Song, H]]		[[Category: Song, H]]
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 06:23, 15 February 2018

OCA — Thu, 15 Feb 2018 06:23:40 GMT

←Older revision		Revision as of 06:23, 15 February 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5n0w is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of OphA-DeltaC6 mutant R72A in complex with SAM==
-		+	<StructureSection load='5n0w' size='340' side='right' caption='[[5n0w]], [[Resolution\|resolution]] 1.93Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5n0w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N0W FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n0w OCA], [http://pdbe.org/5n0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n0w RCSB], [http://www.ebi.ac.uk/pdbsum/5n0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n0w ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Naismith, J H]]
		+	[[Category: Song, H]]
		+	[[Category: Methyltransferase]]
		+	[[Category: Transferase]]

OCA at 07:31, 12 April 2017

OCA — Wed, 12 Apr 2017 07:31:14 GMT

←Older revision		Revision as of 07:31, 12 April 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5n0w is ON HOLD	+	The entry 5n0w is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 17:00, 15 March 2017

OCA — Wed, 15 Mar 2017 17:00:22 GMT

←Older revision		Revision as of 17:00, 15 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5n0w is ON HOLD ~~until Paper Publication~~	+	The entry 5n0w is ON HOLD

	Authors:		Authors:

OCA: Protected "5n0w" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 11:53:19 GMT

Protected "5n0w" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:53, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5n0w is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Thu, 09 Mar 2017 11:53:18 GMT

New page: '''Unreleased structure''' The entry 5n0w is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5n0w is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]