5n2b - Revision history

OCA at 17:58, 8 November 2023

OCA — Wed, 08 Nov 2023 17:58:29 GMT

←Older revision		Revision as of 17:58, 8 November 2023
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	<StructureSection load='5n2b' size='340' side='right'caption='[[5n2b]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5n2b' size='340' side='right'caption='[[5n2b]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n2b]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Burps Burps~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5N2B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n2b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N2B FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=EDO:~~1~~,2-ETHANEDIOL'>EDO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~BPSL1626 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=272560 BURPS])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5n2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2b OCA], [~~http~~://pdbe.org/5n2b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5n2b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5n2b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2b ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2b OCA], [https://pdbe.org/5n2b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n2b RCSB], [https://www.ebi.ac.uk/pdbsum/5n2b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2b ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q63UH6_BURPS Q63UH6_BURPS]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Burps~~]]	+	[[Category: Burkholderia pseudomallei K96243]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bolognesi, M]]	+	[[Category: Bolognesi M]]
-	[[Category: Gourlay~~, L J]]~~	+	[[Category: Gourlay LJ]]
-	~~[[Category: Antigen]]~~	+
-	~~[[Category: Fimbrial subunit]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Incomplete immunoglobulin-like fold]]~~	+
-	~~[[Category: Melioidosis~~]]	+

OCA at 10:55, 2 October 2019

OCA — Wed, 02 Oct 2019 10:55:17 GMT

←Older revision		Revision as of 10:55, 2 October 2019
Line 1:		Line 1:

	==The crystal structure of Burkholderia pseudomallei antigen and type I fimbria protein BPSL1626.==		==The crystal structure of Burkholderia pseudomallei antigen and type I fimbria protein BPSL1626.==
-	<StructureSection load='5n2b' size='340' side='right' caption='[[5n2b]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5n2b' size='340' side='right'caption='[[5n2b]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5n2b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N2B FirstGlance]. <br>		<table><tr><td colspan='2'>[[5n2b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N2B FirstGlance]. <br>
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2b OCA], [http://pdbe.org/5n2b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n2b RCSB], [http://www.ebi.ac.uk/pdbsum/5n2b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2b OCA], [http://pdbe.org/5n2b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n2b RCSB], [http://www.ebi.ac.uk/pdbsum/5n2b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2b ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Due to significant advances in computational biology, protein prediction, together with antigen and epitope design, have rapidly moved from conventional methods, based on experimental approaches, to in silico-based bioinformatics methods. In this context, we report a reverse vaccinology study that identified a panel of 104 candidate antigens from the Gram-negative bacterial pathogen Burkholderia pseudomallei, which is responsible for the disease melioidosis. B. pseudomallei can cause fatal sepsis in endemic populations in the tropical regions of the world and treatment with antibiotics is mostly ineffective. With the aim of identifying potential vaccine candidates, we report the experimental validation of predicted antigen and type I fimbrial subunit, BPSL1626, which we show is able to recognize and bind human antibodies from the sera of Burkholderia infected patients and to stimulate T-lymphocytes in vitro. The prerequisite for a melioidosis vaccine, in fact, is that both antibody- and cell-mediated immune responses must be triggered. In order to reveal potential antigenic regions of the protein that may aid immunogen re-design, we also report the crystal structure of BPSL1626 at 1.9 A resolution on which structure-based epitope predictions were based. Overall, our data suggest that BPSL1626 and three epitope regions here-identified can represent viable candidates as potential antigenic molecules.
		+
		+	BPSL1626: Reverse and Structural Vaccinology Reveal a Novel Candidate for Vaccine Design against Burkholderia pseudomallei.,Capelli R, Peri C, Villa R, Nithichanon A, Conchillo-Sole O, Yero D, Gagni P, Chiari M, Lertmemongkolchai G, Cretich M, Daura X, Bolognesi M, Colombo G, Gourlay LJ Antibodies (Basel). 2018 Jul 19;7(3). pii: antib7030026. doi:, 10.3390/antib7030026. PMID:31544878<ref>PMID:31544878</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5n2b" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Burps]]		[[Category: Burps]]
		+	[[Category: Large Structures]]
	[[Category: Bolognesi, M]]		[[Category: Bolognesi, M]]
	[[Category: Gourlay, L J]]		[[Category: Gourlay, L J]]

OCA at 07:04, 12 December 2018

OCA — Wed, 12 Dec 2018 07:04:22 GMT

←Older revision		Revision as of 07:04, 12 December 2018
Line 3:		Line 3:
	<StructureSection load='5n2b' size='340' side='right' caption='[[5n2b]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5n2b' size='340' side='right' caption='[[5n2b]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n2b]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N2B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n2b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N2B FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BPSL1626 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272560 BURPS])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2b OCA], [http://pdbe.org/5n2b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n2b RCSB], [http://www.ebi.ac.uk/pdbsum/5n2b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2b OCA], [http://pdbe.org/5n2b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n2b RCSB], [http://www.ebi.ac.uk/pdbsum/5n2b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2b ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Burps]]
	[[Category: Bolognesi, M]]		[[Category: Bolognesi, M]]
	[[Category: Gourlay, L J]]		[[Category: Gourlay, L J]]

OCA at 06:49, 17 January 2018

OCA — Wed, 17 Jan 2018 06:49:09 GMT

←Older revision		Revision as of 06:49, 17 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5n2b is ON HOLD~~	+	==The crystal structure of Burkholderia pseudomallei antigen and type I fimbria protein BPSL1626.==
-		+	<StructureSection load='5n2b' size='340' side='right' caption='[[5n2b]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~Authors: Gourlay, L.J., Bolognesi, M.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5n2b]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N2B FirstGlance]. <br>
-	~~Description:~~ The crystal structure of Burkholderia pseudomallei antigen and type I fimbria protein BPSL1626.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2b OCA], [http://pdbe.org/5n2b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n2b RCSB], [http://www.ebi.ac.uk/pdbsum/5n2b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2b ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bolognesi, M]]		[[Category: Bolognesi, M]]
-	[[Category: Gourlay, L.J]]	+	[[Category: Gourlay, L J]]
		+	[[Category: Antigen]]
		+	[[Category: Fimbrial subunit]]
		+	[[Category: Immune system]]
		+	[[Category: Incomplete immunoglobulin-like fold]]
		+	[[Category: Melioidosis]]

OCA: Protected "5n2b" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 11:57:01 GMT

Protected "5n2b" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:57, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5n2b is ON HOLD Authors: Gourlay, L.J., Bolognesi, M. Description: The crystal structure of Burkholderia pseudomallei antigen and type I fimbria pr...

OCA — Thu, 09 Mar 2017 11:56:59 GMT

New page: '''Unreleased structure''' The entry 5n2b is ON HOLD Authors: Gourlay, L.J., Bolognesi, M. Description: The crystal structure of Burkholderia pseudomallei antigen and type I fimbria pr...

New page

'''Unreleased structure'''

The entry 5n2b is ON HOLD

Authors: Gourlay, L.J., Bolognesi, M.

Description: The crystal structure of Burkholderia pseudomallei antigen and type I fimbria protein BPSL1626.
[[Category: Unreleased Structures]]
[[Category: Bolognesi, M]]
[[Category: Gourlay, L.J]]