5n2q - Revision history

OCA at 17:59, 8 November 2023

OCA — Wed, 08 Nov 2023 17:59:04 GMT

←Older revision		Revision as of 17:59, 8 November 2023
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	<StructureSection load='5n2q' size='340' side='right'caption='[[5n2q]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5n2q' size='340' side='right'caption='[[5n2q]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n2q]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/ ] and [~~http~~://en.wikipedia.org/wiki/~~"streptoccocus_de_la_mammite"_nocard_and_mollereau_1887 "streptoccocus de la mammite" nocard and mollereau 1887~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5N2Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n2q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmid_pMV158 Plasmid pMV158] and [https://en.wikipedia.org/wiki/Streptococcus_agalactiae Streptococcus agalactiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N2Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:~~SODIUM+ION~~'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pre, mob ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1311 "Streptoccocus de la mammite" Nocard and Mollereau 1887])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2q OCA], [https://pdbe.org/5n2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n2q RCSB], [https://www.ebi.ac.uk/pdbsum/5n2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2q ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5n2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2q OCA], [~~http~~://pdbe.org/5n2q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5n2q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5n2q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2q ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PRE_STRAG PRE_STRAG]] The interaction of the RSA site and the PRE protein may not only serves a function in plasmid maintenance, but may also contributes to the distribution of small antibiotic resistance plasmids among Gram-positive bacteria.	+	[https://www.uniprot.org/uniprot/PRE_STRAG PRE_STRAG] The interaction of the RSA site and the PRE protein may not only serves a function in plasmid maintenance, but may also contributes to the distribution of small antibiotic resistance plasmids among Gram-positive bacteria.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptoccocus de la mammite nocard and mollereau 1887]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Boer, D R~~]]	+	[[Category: Plasmid pMV158]]
-	[[Category: ~~Coll, M~~]]	+	[[Category: Streptococcus agalactiae]]
-	[[Category: ~~Russi, S~~]]	+	[[Category: Boer DR]]
-	[[Category: ~~Conjugation~~]]	+	[[Category: Coll M]]
-	[[Category: ~~Dna binding protein]]~~	+	[[Category: Russi S]]
-	~~[[Category: Nuclease]]~~	+
-	~~[[Category: Relaxase~~]]	+

OCA at 06:41, 16 October 2019

OCA — Wed, 16 Oct 2019 06:41:51 GMT

←Older revision		Revision as of 06:41, 16 October 2019
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	==MobM Relaxase Domain (MOBV; Mob_Pre) bound to 26nt pMV158 oriT DNA==		==MobM Relaxase Domain (MOBV; Mob_Pre) bound to 26nt pMV158 oriT DNA==
-	<StructureSection load='5n2q' size='340' side='right' caption='[[5n2q]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5n2q' size='340' side='right'caption='[[5n2q]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n2q]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N2Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n2q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/"streptoccocus_de_la_mammite"_nocard_and_mollereau_1887 "streptoccocus de la mammite" nocard and mollereau 1887]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N2Q FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pre, mob ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1311 "Streptoccocus de la mammite" Nocard and Mollereau 1887])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2q OCA], [http://pdbe.org/5n2q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n2q RCSB], [http://www.ebi.ac.uk/pdbsum/5n2q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2q OCA], [http://pdbe.org/5n2q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n2q RCSB], [http://www.ebi.ac.uk/pdbsum/5n2q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2q ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Streptoccocus de la mammite nocard and mollereau 1887]]
		+	[[Category: Large Structures]]
	[[Category: Boer, D R]]		[[Category: Boer, D R]]
	[[Category: Coll, M]]		[[Category: Coll, M]]

OCA at 09:21, 27 September 2017

OCA — Wed, 27 Sep 2017 09:21:32 GMT

←Older revision		Revision as of 09:21, 27 September 2017
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PRE_STRAG PRE_STRAG]] The interaction of the RSA site and the PRE protein may not only serves a function in plasmid maintenance, but may also contributes to the distribution of small antibiotic resistance plasmids among Gram-positive bacteria.		[[http://www.uniprot.org/uniprot/PRE_STRAG PRE_STRAG]] The interaction of the RSA site and the PRE protein may not only serves a function in plasmid maintenance, but may also contributes to the distribution of small antibiotic resistance plasmids among Gram-positive bacteria.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Relaxases are metal-dependent nucleases that break and join DNA for the initiation and completion of conjugative bacterial gene transfer. Conjugation is the main process through which antibiotic resistance spreads among bacteria, with multidrug-resistant staphylococci and streptococci infections posing major threats to human health. The MOBV family of relaxases accounts for approximately 85% of all relaxases found in Staphylococcus aureus isolates. Here, we present six structures of the MOBV relaxase MobM from the promiscuous plasmid pMV158 in complex with several origin of transfer DNA fragments. A combined structural, biochemical, and computational approach reveals that MobM follows a previously uncharacterized histidine/metal-dependent DNA processing mechanism, which involves the formation of a covalent phosphoramidate histidine-DNA adduct for cell-to-cell transfer. We discuss how the chemical features of the high-energy phosphorus-nitrogen bond shape the dominant position of MOBV histidine relaxases among small promiscuous plasmids and their preference toward Gram-positive bacteria.
		+
		+	Structural basis of a histidine-DNA nicking/joining mechanism for gene transfer and promiscuous spread of antibiotic resistance.,Pluta R, Boer DR, Lorenzo-Diaz F, Russi S, Gomez H, Fernandez-Lopez C, Perez-Luque R, Orozco M, Espinosa M, Coll M Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):E6526-E6535. doi:, 10.1073/pnas.1702971114. Epub 2017 Jul 24. PMID:28739894<ref>PMID:28739894</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5n2q" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:46, 12 April 2017

OCA — Wed, 12 Apr 2017 11:46:23 GMT

←Older revision		Revision as of 11:46, 12 April 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5n2q is ~~ON HOLD until Paper Publication~~	+	==MobM Relaxase Domain (MOBV; Mob_Pre) bound to 26nt pMV158 oriT DNA==
-		+	<StructureSection load='5n2q' size='340' side='right' caption='[[5n2q]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~: ~~Russi~~, S., ~~Boer~~, D.R., ~~Coll~~, M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5n2q]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N2Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N2Q FirstGlance]. <br>
-	~~Description~~: ~~MobM Relaxase Domain (MOBV; Mob_Pre) bound~~ to ~~26nt pMV158 oriT DNA~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n2q OCA], [http://pdbe.org/5n2q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n2q RCSB], [http://www.ebi.ac.uk/pdbsum/5n2q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n2q ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRE_STRAG PRE_STRAG]] The interaction of the RSA site and the PRE protein may not only serves a function in plasmid maintenance, but may also contributes to the distribution of small antibiotic resistance plasmids among Gram-positive bacteria.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Boer, D R]]
		+	[[Category: Coll, M]]
	[[Category: Russi, S]]		[[Category: Russi, S]]
-	[[Category: ~~Boer, D.R~~]]	+	[[Category: Conjugation]]
-	[[Category: ~~Coll, M~~]]	+	[[Category: Dna binding protein]]
		+	[[Category: Nuclease]]
		+	[[Category: Relaxase]]

OCA at 17:03, 15 March 2017

OCA — Wed, 15 Mar 2017 17:03:51 GMT

←Older revision		Revision as of 17:03, 15 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5n2q is ON HOLD	+	The entry 5n2q is ON HOLD until Paper Publication

	Authors: Russi, S., Boer, D.R., Coll, M.		Authors: Russi, S., Boer, D.R., Coll, M.

OCA: Protected "5n2q" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 11:58:30 GMT

Protected "5n2q" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:58, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5n2q is ON HOLD Authors: Russi, S., Boer, D.R., Coll, M. Description: MobM Relaxase Domain (MOBV; Mob_Pre) bound to 26nt pMV158 oriT DNA [[Category...

OCA — Thu, 09 Mar 2017 11:58:29 GMT

New page: '''Unreleased structure''' The entry 5n2q is ON HOLD Authors: Russi, S., Boer, D.R., Coll, M. Description: MobM Relaxase Domain (MOBV; Mob_Pre) bound to 26nt pMV158 oriT DNA [[Category...

New page

'''Unreleased structure'''

The entry 5n2q is ON HOLD

Authors: Russi, S., Boer, D.R., Coll, M.

Description: MobM Relaxase Domain (MOBV; Mob_Pre) bound to 26nt pMV158 oriT DNA
[[Category: Unreleased Structures]]
[[Category: Russi, S]]
[[Category: Boer, D.R]]
[[Category: Coll, M]]