5n7x - Revision history

OCA at 12:32, 15 November 2023

OCA — Wed, 15 Nov 2023 12:32:53 GMT

←Older revision		Revision as of 12:32, 15 November 2023
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	<StructureSection load='5n7x' size='340' side='right'caption='[[5n7x]], [[Resolution\|resolution]] 1.12Å' scene=''>		<StructureSection load='5n7x' size='340' side='right'caption='[[5n7x]], [[Resolution\|resolution]] 1.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n7x]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N7X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5N7X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n7x]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N7X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5n7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n7x OCA], [~~http~~://pdbe.org/5n7x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5n7x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5n7x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5n7x ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.12Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n7x OCA], [https://pdbe.org/5n7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n7x RCSB], [https://www.ebi.ac.uk/pdbsum/5n7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n7x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Albert, T]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: Bannen, R]]	+	[[Category: Albert T]]
-	[[Category: Benz, J]]	+	[[Category: Bannen R]]
-	[[Category: Goodrich, L]]	+	[[Category: Benz J]]
-	[[Category: Lyamichev, V]]	+	[[Category: Goodrich L]]
-	[[Category: Patel, J]]	+	[[Category: Lyamichev V]]
-	[[Category: Sullivan, E]]	+	[[Category: Patel J]]
-	~~[[Category: Biotin binding protein]]~~	+	[[Category: Sullivan E]]
-	~~[[Category: Hpq motif]]~~	+
-	~~[[Category: Streptavidin]]~~	+
-	~~[[Category: Streptavidin peptide 11101 complex~~]]	+

OCA at 11:58, 26 August 2020

OCA — Wed, 26 Aug 2020 11:58:36 GMT

←Older revision		Revision as of 11:58, 26 August 2020
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	==CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE EWVHPQFEQKAK==		==CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE EWVHPQFEQKAK==
-	<StructureSection load='5n7x' size='340' side='right' caption='[[5n7x]], [[Resolution\|resolution]] 1.12Å' scene=''>	+	<StructureSection load='5n7x' size='340' side='right'caption='[[5n7x]], [[Resolution\|resolution]] 1.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n7x]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N7X OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5N7X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n7x]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N7X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N7X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5n7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n7x OCA], [http://pdbe.org/5n7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n7x RCSB], [http://www.ebi.ac.uk/pdbsum/5n7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n7x ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n7x OCA], [http://pdbe.org/5n7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n7x RCSB], [http://www.ebi.ac.uk/pdbsum/5n7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n7x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5n7x" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5n7x" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Albert, T]]		[[Category: Albert, T]]
	[[Category: Bannen, R]]		[[Category: Bannen, R]]

OCA at 09:31, 4 October 2017

OCA — Wed, 04 Oct 2017 09:31:34 GMT

←Older revision		Revision as of 09:31, 4 October 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5n7x is ~~ON HOLD~~	+	==CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE EWVHPQFEQKAK==
		+	<StructureSection load='5n7x' size='340' side='right' caption='[[5n7x]], [[Resolution\|resolution]] 1.12Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5n7x]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N7X FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n7x OCA], [http://pdbe.org/5n7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n7x RCSB], [http://www.ebi.ac.uk/pdbsum/5n7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n7x ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Considerable efforts have been made to develop technologies for selection of peptidic molecules that act as substrates or binders to a protein of interest. Here we demonstrate the combination of rational peptide array library design, parallel screening and stepwise evolution, to discover novel peptide hotspots. These hotspots can be systematically evolved to create high-affinity, high-specificity binding peptides to a protein target in a reproducible and digitally controlled process. The method can be applied to synthesize both linear and cyclic peptides, as well as peptides composed of natural and non-natural amino acid analogs, thereby enabling screens in a much diverse chemical space. We apply this method to stepwise evolve peptide binders to streptavidin, a protein studied for over two decades and report novel peptides that mimic key interactions of biotin to streptavidin.

-	~~Authors:~~ Lyamichev~~, V.~~, Goodrich~~, L.~~, Sullivan~~, E.~~, Bannen~~, R.~~, Benz, J., Albert~~, T.~~, Patel~~, J~~.	+	Stepwise Evolution Improves Identification of Diverse Peptides Binding to a Protein Target.,Lyamichev VI, Goodrich LE, Sullivan EH, Bannen RM, Benz J, Albert TJ, Patel JJ Sci Rep. 2017 Sep 21;7(1):12116. doi: 10.1038/s41598-017-12440-1. PMID:28935886<ref>PMID:28935886</ref>

-	~~Description: CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE EWVHPQFEQKAK~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Lyamichev, V]]~~	+	<div class="pdbe-citations 5n7x" style="background-color:#fffaf0;"></div>
-	~~[[Category: Goodrich, L]]~~	+	== References ==
-	~~[[Category: Sullivan, E]]~~	+	<references/>
-	[[Category: ~~Benz~~, J]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Albert, T]]
	[[Category: Bannen, R]]		[[Category: Bannen, R]]
		+	[[Category: Benz, J]]
		+	[[Category: Goodrich, L]]
		+	[[Category: Lyamichev, V]]
	[[Category: Patel, J]]		[[Category: Patel, J]]
-	[[Category: ~~Albert~~, T]]	+	[[Category: Sullivan, E]]
		+	[[Category: Biotin binding protein]]
		+	[[Category: Hpq motif]]
		+	[[Category: Streptavidin]]
		+	[[Category: Streptavidin peptide 11101 complex]]

OCA: Protected "5n7x" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 12:14:03 GMT

Protected "5n7x" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:14, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5n7x is ON HOLD Authors: Lyamichev, V., Goodrich, L., Sullivan, E., Bannen, R., Benz, J., Albert, T., Patel, J. Description: CRYSTAL STRUCTURE OF S...

OCA — Thu, 09 Mar 2017 12:14:01 GMT

New page: '''Unreleased structure''' The entry 5n7x is ON HOLD Authors: Lyamichev, V., Goodrich, L., Sullivan, E., Bannen, R., Benz, J., Albert, T., Patel, J. Description: CRYSTAL STRUCTURE OF S...

New page

'''Unreleased structure'''

The entry 5n7x is ON HOLD

Authors: Lyamichev, V., Goodrich, L., Sullivan, E., Bannen, R., Benz, J., Albert, T., Patel, J.

Description: CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE EWVHPQFEQKAK
[[Category: Unreleased Structures]]
[[Category: Lyamichev, V]]
[[Category: Goodrich, L]]
[[Category: Sullivan, E]]
[[Category: Benz, J]]
[[Category: Bannen, R]]
[[Category: Patel, J]]
[[Category: Albert, T]]