5n99 - Revision history

OCA at 12:38, 15 November 2023

OCA — Wed, 15 Nov 2023 12:38:06 GMT

←Older revision		Revision as of 12:38, 15 November 2023
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	<StructureSection load='5n99' size='340' side='right'caption='[[5n99]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='5n99' size='340' side='right'caption='[[5n99]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n99]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N99 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5N99 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n99]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N99 FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5n99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n99 OCA], [~~http~~://pdbe.org/5n99 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5n99 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5n99 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5n99 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n99 OCA], [https://pdbe.org/5n99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n99 RCSB], [https://www.ebi.ac.uk/pdbsum/5n99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n99 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Albert, T~~]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: ~~Bannen, R~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Benz, J~~]]	+	[[Category: Albert T]]
-	[[Category: ~~Goodrich, L~~]]	+	[[Category: Bannen R]]
-	[[Category: ~~Lyamichev, V]]~~	+	[[Category: Benz J]]
-	~~[[Category: Patel,~~ J]]	+	[[Category: Goodrich L]]
-	[[Category: ~~Sullivan, E~~]]	+	[[Category: Lyamichev V]]
-	[[Category: ~~Biotin binding~~]]	+	[[Category: Patel J]]
-	[[Category: ~~Biotin binding protein~~]]	+	[[Category: Sullivan E]]
-	[[Category: ~~D-amino acid]]~~	+
-	~~[[Category: Streptavidin]]~~	+
-	~~[[Category: Streptavidin cyclic peptide complex~~]]	+

OCA at 11:59, 26 August 2020

OCA — Wed, 26 Aug 2020 11:59:43 GMT

←Older revision		Revision as of 11:59, 26 August 2020
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	==CRYSTAL STRUCTURE OF STREPTAVIDIN with cyclic peptide NQpWQ==		==CRYSTAL STRUCTURE OF STREPTAVIDIN with cyclic peptide NQpWQ==
-	<StructureSection load='5n99' size='340' side='right' caption='[[5n99]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='5n99' size='340' side='right'caption='[[5n99]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n99]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N99 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5N99 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n99]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N99 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N99 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5n99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n99 OCA], [http://pdbe.org/5n99 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n99 RCSB], [http://www.ebi.ac.uk/pdbsum/5n99 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n99 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n99 OCA], [http://pdbe.org/5n99 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n99 RCSB], [http://www.ebi.ac.uk/pdbsum/5n99 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n99 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5n99" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5n99" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Albert, T]]		[[Category: Albert, T]]
	[[Category: Bannen, R]]		[[Category: Bannen, R]]

OCA at 09:23, 4 October 2017

OCA — Wed, 04 Oct 2017 09:23:02 GMT

←Older revision		Revision as of 09:23, 4 October 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5n99 is ~~ON HOLD~~	+	==CRYSTAL STRUCTURE OF STREPTAVIDIN with cyclic peptide NQpWQ==
		+	<StructureSection load='5n99' size='340' side='right' caption='[[5n99]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5n99]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N99 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N99 FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n99 OCA], [http://pdbe.org/5n99 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n99 RCSB], [http://www.ebi.ac.uk/pdbsum/5n99 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n99 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Considerable efforts have been made to develop technologies for selection of peptidic molecules that act as substrates or binders to a protein of interest. Here we demonstrate the combination of rational peptide array library design, parallel screening and stepwise evolution, to discover novel peptide hotspots. These hotspots can be systematically evolved to create high-affinity, high-specificity binding peptides to a protein target in a reproducible and digitally controlled process. The method can be applied to synthesize both linear and cyclic peptides, as well as peptides composed of natural and non-natural amino acid analogs, thereby enabling screens in a much diverse chemical space. We apply this method to stepwise evolve peptide binders to streptavidin, a protein studied for over two decades and report novel peptides that mimic key interactions of biotin to streptavidin.

-	~~Authors:~~ Lyamichev~~, V.~~, Goodrich~~, L.~~, Sullivan~~, E.~~, Bannen~~, R.~~, Benz, J., Albert~~, T.~~, Patel~~, J~~.	+	Stepwise Evolution Improves Identification of Diverse Peptides Binding to a Protein Target.,Lyamichev VI, Goodrich LE, Sullivan EH, Bannen RM, Benz J, Albert TJ, Patel JJ Sci Rep. 2017 Sep 21;7(1):12116. doi: 10.1038/s41598-017-12440-1. PMID:28935886<ref>PMID:28935886</ref>

-	~~Description: CRYSTAL STRUCTURE OF STREPTAVIDIN with cyclic peptide NQpWQ~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Lyamichev, V]]~~	+	<div class="pdbe-citations 5n99" style="background-color:#fffaf0;"></div>
-	~~[[Category: Goodrich, L]]~~	+	== References ==
-	~~[[Category: Sullivan, E]]~~	+	<references/>
-	[[Category: ~~Benz~~, J]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Albert, T]]
	[[Category: Bannen, R]]		[[Category: Bannen, R]]
		+	[[Category: Benz, J]]
		+	[[Category: Goodrich, L]]
		+	[[Category: Lyamichev, V]]
	[[Category: Patel, J]]		[[Category: Patel, J]]
-	[[Category: ~~Albert~~, T]]	+	[[Category: Sullivan, E]]
		+	[[Category: Biotin binding]]
		+	[[Category: Biotin binding protein]]
		+	[[Category: D-amino acid]]
		+	[[Category: Streptavidin]]
		+	[[Category: Streptavidin cyclic peptide complex]]

OCA: Protected "5n99" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 12:18:29 GMT

Protected "5n99" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:18, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5n99 is ON HOLD Authors: Lyamichev, V., Goodrich, L., Sullivan, E., Bannen, R., Benz, J., Albert, T., Patel, J. Description: CRYSTAL STRUCTURE OF S...

OCA — Thu, 09 Mar 2017 12:18:28 GMT

New page: '''Unreleased structure''' The entry 5n99 is ON HOLD Authors: Lyamichev, V., Goodrich, L., Sullivan, E., Bannen, R., Benz, J., Albert, T., Patel, J. Description: CRYSTAL STRUCTURE OF S...

New page

'''Unreleased structure'''

The entry 5n99 is ON HOLD

Authors: Lyamichev, V., Goodrich, L., Sullivan, E., Bannen, R., Benz, J., Albert, T., Patel, J.

Description: CRYSTAL STRUCTURE OF STREPTAVIDIN with cyclic peptide NQpWQ
[[Category: Unreleased Structures]]
[[Category: Lyamichev, V]]
[[Category: Goodrich, L]]
[[Category: Sullivan, E]]
[[Category: Benz, J]]
[[Category: Bannen, R]]
[[Category: Patel, J]]
[[Category: Albert, T]]