5n9u - Revision history

OCA at 12:40, 15 November 2023

OCA — Wed, 15 Nov 2023 12:40:00 GMT

←Older revision		Revision as of 12:40, 15 November 2023
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	==Dehydroascorbate reductase 3A from Populus trichocarpa complexed with GSH.==		==Dehydroascorbate reductase 3A from Populus trichocarpa complexed with GSH.==
-	<StructureSection load='5n9u' size='340' side='right'caption='[[5n9u~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='5n9u' size='340' side='right'caption='[[5n9u]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n9u]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Poptr Poptr~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5N9U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n9u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_trichocarpa Populus trichocarpa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N9U FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=GSH:GLUTATHIONE'>GSH</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~POPTR_0008s04920g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=3694 POPTR])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5n9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9u OCA], [~~http~~://pdbe.org/5n9u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5n9u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5n9u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9u OCA], [https://pdbe.org/5n9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n9u RCSB], [https://www.ebi.ac.uk/pdbsum/5n9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B9HM36_POPTR B9HM36_POPTR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Poptr~~]]	+	[[Category: Populus trichocarpa]]
-	[[Category: Roret, T]]	+	[[Category: Roret T]]
-	[[Category: Tsan, P]]	+	[[Category: Tsan P]]
-	~~[[Category: Dehydroascorbate reductase]]~~	+
-	~~[[Category: Glutathione transferase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Plant~~]]	+

OCA at 13:12, 10 May 2019

OCA — Fri, 10 May 2019 13:12:54 GMT

←Older revision		Revision as of 13:12, 10 May 2019
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	==Dehydroascorbate reductase 3A from Populus trichocarpa complexed with GSH.==		==Dehydroascorbate reductase 3A from Populus trichocarpa complexed with GSH.==
-	<StructureSection load='5n9u' size='340' side='right' caption='[[5n9u]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='5n9u' size='340' side='right'caption='[[5n9u]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n9u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N9U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n9u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Poptr Poptr]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N9U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POPTR_0008s04920g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3694 POPTR])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9u OCA], [http://pdbe.org/5n9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n9u RCSB], [http://www.ebi.ac.uk/pdbsum/5n9u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9u OCA], [http://pdbe.org/5n9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n9u RCSB], [http://www.ebi.ac.uk/pdbsum/5n9u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9u ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Poptr]]
	[[Category: Roret, T]]		[[Category: Roret, T]]
	[[Category: Tsan, P]]		[[Category: Tsan, P]]

OCA: Protected "5n9u" [edit=sysop:move=sysop]

OCA — Thu, 09 Mar 2017 16:51:01 GMT

Protected "5n9u" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:51, 9 March 2017

OCA: New page: ==Dehydroascorbate reductase 3A from Populus trichocarpa complexed with GSH.== == Structural highlights ==
<table><tr><td colspan='2'>[[5n9u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N9U FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9u OCA], [http://pdbe.org/5n9u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n9u RCSB], [http://www.ebi.ac.uk/pdbsum/5n9u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9u ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Dehydroascorbate reductases (DHARs), enzymes belonging to the GST superfamily, catalyse the GSH-dependent reduction of dehydroascorbate into ascorbate in plants. By maintaining a reduced ascorbate pool, they notably participate to H2O2 detoxification catalysed by ascorbate peroxidases (APXs). Despite this central role, the catalytic mechanism used by DHARs is still not well understood and there is no supportive 3D structure. In this context, we have performed a thorough biochemical and structural analysis of the three poplar DHARs and coupled this to the analysis of their transcript expression patterns and subcellular localizations. The transcripts for these genes are mainly detected in reproductive and green organs and the corresponding proteins are expressed in plastids, in the cytosol and in the nucleus, but not in mitochondria and peroxisomes where ascorbate regeneration is obviously necessary. Comparing the kinetic properties and the sensitivity to GSSG-mediated oxidation of DHAR2 and DHAR3A, exhibiting 1 or 3 cysteinyl residues respectively, we observed that the presence of additional cysteines in DHAR3A modifies the regeneration mechanism of the catalytic cysteine by forming different redox states. Finally, from the 3D structure of DHAR3A solved by NMR, we were able to map the residues important for the binding of both substrates (GSH and DHA), showing that DHAR active site is very selective for DHA recognition and providing further insights into the catalytic mechanism and the roles of the additional cysteines found in some DHARs.

Insights into ascorbate regeneration in plants: investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpa.,Lallement PA, Roret T, Tsan P, Gualberto JM, Girardet JM, Didierjean C, Rouhier N, Hecker A Biochem J. 2016 Mar 15;473(6):717-31. doi: 10.1042/BJ20151147. Epub 2015 Dec 23. PMID:26699905<ref>PMID:26699905</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5n9u" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Roret, T]]
[[Category: Tsan, P]]
[[Category: Dehydroascorbate reductase]]
[[Category: Glutathione transferase]]
[[Category: Oxidoreductase]]
[[Category: Plant]]