5nhw - Revision history

OCA at 12:58, 15 November 2023

OCA — Wed, 15 Nov 2023 12:58:44 GMT

←Older revision		Revision as of 12:58, 15 November 2023
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	<StructureSection load='5nhw' size='340' side='right'caption='[[5nhw]], [[Resolution\|resolution]] 1.78Å' scene=''>		<StructureSection load='5nhw' size='340' side='right'caption='[[5nhw]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nhw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NHW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NHW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nhw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NHW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=GOL:GLYCEROL'>GOL</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.78Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nhw OCA], [~~http~~://pdbe.org/5nhw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nhw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nhw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nhw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nhw OCA], [https://pdbe.org/5nhw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nhw RCSB], [https://www.ebi.ac.uk/pdbsum/5nhw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nhw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Rondeau, J M]]	+	[[Category: Rondeau J-M]]
-	~~[[Category: Anti-activin receptor type-2 antibody]]~~	+
-	~~[[Category: Antibody fab fragment]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin~~]]	+

OCA at 07:47, 11 March 2020

OCA — Wed, 11 Mar 2020 07:47:52 GMT

←Older revision		Revision as of 07:47, 11 March 2020
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	==CRYSTAL STRUCTURE OF THE BIMAGRUMAB Fab==		==CRYSTAL STRUCTURE OF THE BIMAGRUMAB Fab==
-	<StructureSection load='5nhw' size='340' side='right' caption='[[5nhw]], [[Resolution\|resolution]] 1.78Å' scene=''>	+	<StructureSection load='5nhw' size='340' side='right'caption='[[5nhw]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5nhw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NHW FirstGlance]. <br>		<table><tr><td colspan='2'>[[5nhw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NHW FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Rondeau, J M]]		[[Category: Rondeau, J M]]
	[[Category: Anti-activin receptor type-2 antibody]]		[[Category: Anti-activin receptor type-2 antibody]]

OCA at 07:28, 29 November 2017

OCA — Wed, 29 Nov 2017 07:28:01 GMT

←Older revision		Revision as of 07:28, 29 November 2017
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	<StructureSection load='5nhw' size='340' side='right' caption='[[5nhw]], [[Resolution\|resolution]] 1.78Å' scene=''>		<StructureSection load='5nhw' size='340' side='right' caption='[[5nhw]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nhw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NHW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nhw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NHW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Rondeau, J M]]		[[Category: Rondeau, J M]]
	[[Category: Anti-activin receptor type-2 antibody]]		[[Category: Anti-activin receptor type-2 antibody]]

OCA at 07:27, 15 November 2017

OCA — Wed, 15 Nov 2017 07:27:11 GMT

←Older revision		Revision as of 07:27, 15 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5nhw is ~~ON HOLD until Paper~~ Publication	+	==CRYSTAL STRUCTURE OF THE BIMAGRUMAB Fab==
		+	<StructureSection load='5nhw' size='340' side='right' caption='[[5nhw]], [[Resolution\|resolution]] 1.78Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5nhw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NHW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nhw OCA], [http://pdbe.org/5nhw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nhw RCSB], [http://www.ebi.ac.uk/pdbsum/5nhw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nhw ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The TGF-beta family ligands myostatin, GDF11, and activins are negative regulators of skeletal muscle mass, which have been reported to primarily signal via the ActRIIB receptor on skeletal muscle and thereby induce muscle wasting described as cachexia. Use of a soluble ActRIIB-Fc "trap," to block myostatin pathway signaling in normal or cachectic mice leads to hypertrophy or prevention of muscle loss, perhaps suggesting that the ActRIIB receptor is primarily responsible for muscle growth regulation. Genetic evidence demonstrates however that both ActRIIB- and ActRIIA-deficient mice display a hypertrophic phenotype. Here, we describe the mode of action of bimagrumab (BYM338), as a human dual-specific anti-ActRIIA/ActRIIB antibody, at the molecular and cellular levels. As shown by X-ray analysis, bimagrumab binds to both ActRIIA and ActRIIB ligand binding domains in a competitive manner at the critical myostatin/activin binding site, hence preventing signal transduction through either ActRII. Myostatin and the activins are capable of binding to both ActRIIA and ActRIIB, with different affinities. However, blockade of either single receptor through the use of specific anti-ActRIIA or anti-ActRIIB antibodies achieves only a partial signaling blockade upon myostatin or activin A stimulation, and this leads to only a small increase in muscle mass. Complete neutralization and maximal anabolic response are achieved only by simultaneous blockade of both receptors. These findings demonstrate the importance of ActRIIA in addition to ActRIIB in mediating myostatin and activin signaling and highlight the need for blocking both receptors to achieve a strong functional benefit.

-	~~Authors:~~ Rondeau, ~~J.-~~M.	+	Blockade of activin type II receptors with a dual anti-ActRIIA/IIB antibody is critical to promote maximal skeletal muscle hypertrophy.,Morvan F, Rondeau JM, Zou C, Minetti G, Scheufler C, Scharenberg M, Jacobi C, Brebbia P, Ritter V, Toussaint G, Koelbing C, Leber X, Schilb A, Witte F, Lehmann S, Koch E, Geisse S, Glass DJ, Lach-Trifilieff E Proc Natl Acad Sci U S A. 2017 Nov 6. pii: 201707925. doi:, 10.1073/pnas.1707925114. PMID:29109273<ref>PMID:29109273</ref>

-	~~Description~~: ~~CRYSTAL STRUCTURE OF THE BIMAGRUMAB Fab~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Rondeau, J.~~-M]]	+	<div class="pdbe-citations 5nhw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Rondeau, J M]]
		+	[[Category: Anti-activin receptor type-2 antibody]]
		+	[[Category: Antibody fab fragment]]
		+	[[Category: Immune system]]
		+	[[Category: Immunoglobulin]]

OCA: Protected "5nhw" [edit=sysop:move=sysop]

OCA — Wed, 05 Apr 2017 10:09:30 GMT

Protected "5nhw" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:09, 5 April 2017

OCA: New page: '''Unreleased structure''' The entry 5nhw is ON HOLD until Paper Publication Authors: Rondeau, J.-M. Description: CRYSTAL STRUCTURE OF THE BIMAGRUMAB Fab [[Category: Unreleased Structu...

OCA — Wed, 05 Apr 2017 10:09:29 GMT

New page: '''Unreleased structure''' The entry 5nhw is ON HOLD until Paper Publication Authors: Rondeau, J.-M. Description: CRYSTAL STRUCTURE OF THE BIMAGRUMAB Fab [[Category: Unreleased Structu...

New page

'''Unreleased structure'''

The entry 5nhw is ON HOLD until Paper Publication

Authors: Rondeau, J.-M.

Description: CRYSTAL STRUCTURE OF THE BIMAGRUMAB Fab
[[Category: Unreleased Structures]]
[[Category: Rondeau, J.-M]]