5nj4 - Revision history

OCA at 13:01, 15 November 2023

OCA — Wed, 15 Nov 2023 13:01:16 GMT

←Older revision		Revision as of 13:01, 15 November 2023
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	<StructureSection load='5nj4' size='340' side='right'caption='[[5nj4]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5nj4' size='340' side='right'caption='[[5nj4]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nj4]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5NJ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nj4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NJ4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4~~</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME~~</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5nj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj4 OCA], [~~http~~://pdbe.org/5nj4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nj4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nj4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj4 OCA], [https://pdbe.org/5nj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nj4 RCSB], [https://www.ebi.ac.uk/pdbsum/5nj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/RCEM_BLAVI RCEM_BLAVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/CYCR_BLAVI CYCR_BLAVI]] The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.<ref>PMID:10736158</ref> [[http://www.uniprot.org/uniprot/RCEL_BLAVI RCEL_BLAVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/RCEH_BLAVI RCEH_BLAVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.	+	[https://www.uniprot.org/uniprot/RCEL_BLAVI RCEL_BLAVI] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	[[Category: Blastochloris viridis]]		[[Category: Blastochloris viridis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baath, P]]	+	[[Category: Baath P]]
-	[[Category: Branden, G]]	+	[[Category: Branden G]]
-	[[Category: Dods, R]]	+	[[Category: Dods R]]
-	[[Category: Neutze, R]]	+	[[Category: Neutze R]]
-	~~[[Category: Membrane photosynthetic wild-type xfel]]~~	+
-	~~[[Category: Membrane protein~~]]	+

OCA at 12:05, 26 August 2020

OCA — Wed, 26 Aug 2020 12:05:55 GMT

←Older revision		Revision as of 12:05, 26 August 2020
Line 1:		Line 1:

	==From macrocrystals to microcrystals: a strategy for membrane protein serial crystallography==		==From macrocrystals to microcrystals: a strategy for membrane protein serial crystallography==
-	<StructureSection load='5nj4' size='340' side='right' caption='[[5nj4]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5nj4' size='340' side='right'caption='[[5nj4]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nj4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NJ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nj4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NJ4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj4 OCA], [http://pdbe.org/5nj4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nj4 RCSB], [http://www.ebi.ac.uk/pdbsum/5nj4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5nj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj4 OCA], [http://pdbe.org/5nj4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nj4 RCSB], [http://www.ebi.ac.uk/pdbsum/5nj4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Blastochloris viridis]]		[[Category: Blastochloris viridis]]
		+	[[Category: Large Structures]]
	[[Category: Baath, P]]		[[Category: Baath, P]]
	[[Category: Branden, G]]		[[Category: Branden, G]]

OCA at 06:02, 17 August 2017

OCA — Thu, 17 Aug 2017 06:02:16 GMT

←Older revision		Revision as of 06:02, 17 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5nj4 is ~~ON HOLD~~	+	==From macrocrystals to microcrystals: a strategy for membrane protein serial crystallography==
		+	<StructureSection load='5nj4' size='340' side='right' caption='[[5nj4]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5nj4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NJ4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj4 OCA], [http://pdbe.org/5nj4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nj4 RCSB], [http://www.ebi.ac.uk/pdbsum/5nj4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj4 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RCEM_BLAVI RCEM_BLAVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/CYCR_BLAVI CYCR_BLAVI]] The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.<ref>PMID:10736158</ref> [[http://www.uniprot.org/uniprot/RCEL_BLAVI RCEL_BLAVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/RCEH_BLAVI RCEH_BLAVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serial protein crystallography was developed at X-ray free-electron lasers (XFELs) and is now also being applied at storage ring facilities. Robust strategies for the growth and optimization of microcrystals are needed to advance the field. Here we illustrate a generic strategy for recovering high-density homogeneous samples of microcrystals starting from conditions known to yield large (macro) crystals of the photosynthetic reaction center of Blastochloris viridis (RCvir). We first crushed these crystals prior to multiple rounds of microseeding. Each cycle of microseeding facilitated improvements in the RCvir serial femtosecond crystallography (SFX) structure from 3.3-A to 2.4-A resolution. This approach may allow known crystallization conditions for other proteins to be adapted to exploit novel scientific opportunities created by serial crystallography.

-	~~Authors~~:	+	From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography.,Dods R, Bath P, Arnlund D, Beyerlein KR, Nelson G, Liang M, Harimoorthy R, Berntsen P, Malmerberg E, Johansson L, Andersson R, Bosman R, Carbajo S, Claesson E, Conrad CE, Dahl P, Hammarin G, Hunter MS, Li C, Lisova S, Milathianaki D, Robinson J, Safari C, Sharma A, Williams G, Wickstrand C, Yefanov O, Davidsson J, DePonte DP, Barty A, Branden G, Neutze R Structure. 2017 Jul 27. pii: S0969-2126(17)30217-4. doi:, 10.1016/j.str.2017.07.002. PMID:28781082<ref>PMID:28781082</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5nj4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Blastochloris viridis]]
		+	[[Category: Baath, P]]
		+	[[Category: Branden, G]]
		+	[[Category: Dods, R]]
		+	[[Category: Neutze, R]]
		+	[[Category: Membrane photosynthetic wild-type xfel]]
		+	[[Category: Membrane protein]]

OCA: Protected "5nj4" [edit=sysop:move=sysop]

OCA — Wed, 05 Apr 2017 10:12:28 GMT

Protected "5nj4" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:12, 5 April 2017

OCA: New page: '''Unreleased structure''' The entry 5nj4 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 05 Apr 2017 10:12:26 GMT

New page: '''Unreleased structure''' The entry 5nj4 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5nj4 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]