5nj8 - Revision history

OCA at 13:01, 15 November 2023

OCA — Wed, 15 Nov 2023 13:01:22 GMT

←Older revision		Revision as of 13:01, 15 November 2023
Line 3:		Line 3:
	<StructureSection load='5nj8' size='340' side='right'caption='[[5nj8]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='5nj8' size='340' side='right'caption='[[5nj8]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nj8]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NJ8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nj8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NJ8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=ACT:ACETATE+ION'>ACT</scene~~>, ~~<scene name='pdbligand=ER3:ERBIUM+(III)+ION'>ER3</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~AHR, BHLHE76 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN]), Arnt ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ER3:ERBIUM+(III)+ION'>ER3</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj8 OCA], [~~http~~://pdbe.org/5nj8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nj8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nj8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj8 OCA], [https://pdbe.org/5nj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nj8 RCSB], [https://www.ebi.ac.uk/pdbsum/5nj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/AHR_HUMAN AHR_HUMAN]] Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.<ref>PMID:10395741</ref> <ref>PMID:7961644</ref> [[http://www.uniprot.org/uniprot/ARNT_MOUSE ARNT_MOUSE]] Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).	+	[https://www.uniprot.org/uniprot/AHR_HUMAN AHR_HUMAN] Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.<ref>PMID:10395741</ref> <ref>PMID:7961644</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5nj8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5nj8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[3D structures of hypoxia-inducible factor\|3D structures of hypoxia-inducible factor]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Daumke, O~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Schulte, K W~~]]	+	[[Category: Daumke O]]
-	[[Category: ~~Basic helix loop helix pas domain transcription factor]]~~	+	[[Category: Schulte KW]]
-	~~[[Category: Transcription~~]]	+

OCA at 08:57, 5 February 2020

OCA — Wed, 05 Feb 2020 08:57:03 GMT

←Older revision		Revision as of 08:57, 5 February 2020
Line 1:		Line 1:

	==Structural basis for aryl hydrocarbon receptor mediated gene activation==		==Structural basis for aryl hydrocarbon receptor mediated gene activation==
-	<StructureSection load='5nj8' size='340' side='right' caption='[[5nj8]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<StructureSection load='5nj8' size='340' side='right'caption='[[5nj8]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nj8]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NJ8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nj8]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NJ8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ER3:ERBIUM+(III)+ION'>ER3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ER3:ERBIUM+(III)+ION'>ER3</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AHR, BHLHE76 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), Arnt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj8 OCA], [http://pdbe.org/5nj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nj8 RCSB], [http://www.ebi.ac.uk/pdbsum/5nj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj8 OCA], [http://pdbe.org/5nj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nj8 RCSB], [http://www.ebi.ac.uk/pdbsum/5nj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/AHR_HUMAN AHR_HUMAN]] Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.<ref>PMID:10395741</ref> <ref>PMID:7961644</ref> [[http://www.uniprot.org/uniprot/~~ARNT_HUMAN ARNT_HUMAN~~]] Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.	+	[[http://www.uniprot.org/uniprot/AHR_HUMAN AHR_HUMAN]] Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.<ref>PMID:10395741</ref> <ref>PMID:7961644</ref> [[http://www.uniprot.org/uniprot/ARNT_MOUSE ARNT_MOUSE]] Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Daumke, O]]		[[Category: Daumke, O]]
	[[Category: Schulte, K W]]		[[Category: Schulte, K W]]
	[[Category: Basic helix loop helix pas domain transcription factor]]		[[Category: Basic helix loop helix pas domain transcription factor]]
	[[Category: Transcription]]		[[Category: Transcription]]

OCA at 16:45, 20 October 2017

OCA — Fri, 20 Oct 2017 16:45:44 GMT

←Older revision		Revision as of 16:45, 20 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5nj8 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structural basis for aryl hydrocarbon receptor mediated gene activation==
		+	<StructureSection load='5nj8' size='340' side='right' caption='[[5nj8]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5nj8]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NJ8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ER3:ERBIUM+(III)+ION'>ER3</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj8 OCA], [http://pdbe.org/5nj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nj8 RCSB], [http://www.ebi.ac.uk/pdbsum/5nj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AHR_HUMAN AHR_HUMAN]] Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.<ref>PMID:10395741</ref> <ref>PMID:7961644</ref> [[http://www.uniprot.org/uniprot/ARNT_HUMAN ARNT_HUMAN]] Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The aryl hydrocarbon receptor (AHR) and the AHR nuclear translocator (ARNT) constitute a heterodimeric basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS) domain containing transcription factor with central functions in development and cellular homeostasis. AHR is activated by xenobiotics, notably dioxin, as well as by exogenous and endogenous metabolites. Modulation of AHR activity holds promise for the treatment of diseases featuring altered cellular homeostasis, such as cancer or autoimmune disorders. Here, we present the crystal structure of a heterodimeric AHR:ARNT complex containing the PAS A and bHLH domain bound to its target DNA. The structure provides insights into the DNA binding mode of AHR and elucidates how stable dimerization of AHR:ARNT is achieved through sophisticated domain interplay via three specific interfaces. Using mutational analyses, we prove the relevance of the observed interfaces for AHR-mediated gene activation. Thus, our work establishes the structural basis of AHR assembly and DNA interaction and provides a template for targeted drug design.

-	~~Authors: Daumke, O~~., Schulte, K.W.	+	Structural Basis for Aryl Hydrocarbon Receptor-Mediated Gene Activation.,Schulte KW, Green E, Wilz A, Platten M, Daumke O Structure. 2017 Jul 5;25(7):1025-1033.e3. doi: 10.1016/j.str.2017.05.008. Epub, 2017 Jun 9. PMID:28602820<ref>PMID:28602820</ref>

-	~~Description: Structural basis for aryl hydrocarbon receptor mediated gene activation~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Schulte, K.W]]~~	+	<div class="pdbe-citations 5nj8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Daumke, O]]		[[Category: Daumke, O]]
		+	[[Category: Schulte, K W]]
		+	[[Category: Basic helix loop helix pas domain transcription factor]]
		+	[[Category: Transcription]]

OCA: Protected "5nj8" [edit=sysop:move=sysop]

OCA — Wed, 05 Apr 2017 10:12:38 GMT

Protected "5nj8" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:12, 5 April 2017

OCA: New page: '''Unreleased structure''' The entry 5nj8 is ON HOLD until Paper Publication Authors: Daumke, O., Schulte, K.W. Description: Structural basis for aryl hydrocarbon receptor mediated gen...

OCA — Wed, 05 Apr 2017 10:12:37 GMT

New page: '''Unreleased structure''' The entry 5nj8 is ON HOLD until Paper Publication Authors: Daumke, O., Schulte, K.W. Description: Structural basis for aryl hydrocarbon receptor mediated gen...

New page

'''Unreleased structure'''

The entry 5nj8 is ON HOLD until Paper Publication

Authors: Daumke, O., Schulte, K.W.

Description: Structural basis for aryl hydrocarbon receptor mediated gene activation
[[Category: Unreleased Structures]]
[[Category: Schulte, K.W]]
[[Category: Daumke, O]]