5nj9 - Revision history

OCA at 13:01, 15 November 2023

OCA — Wed, 15 Nov 2023 13:01:26 GMT

←Older revision		Revision as of 13:01, 15 November 2023
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	<StructureSection load='5nj9' size='340' side='right'caption='[[5nj9]], [[Resolution\|resolution]] 1.25Å' scene=''>		<StructureSection load='5nj9' size='340' side='right'caption='[[5nj9]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nj9]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5NJ9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nj9]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NJ9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.25Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5nj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj9 OCA], [~~http~~://pdbe.org/5nj9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nj9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nj9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj9 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj9 OCA], [https://pdbe.org/5nj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nj9 RCSB], [https://www.ebi.ac.uk/pdbsum/5nj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TLDD_ECOLI TLDD_ECOLI]] Metalloprotease involved in CcdA degradation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA).<ref>PMID:12029038</ref> [[http://www.uniprot.org/uniprot/PMBA_ECOLI PMBA_ECOLI]] Metalloprotease involved in CcdA degradation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA).<ref>PMID:12029038</ref>	+	[https://www.uniprot.org/uniprot/TLDD_ECOLI TLDD_ECOLI] Metalloprotease involved in CcdA degradation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA).<ref>PMID:12029038</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ghilarov, D]]	+	[[Category: Ghilarov D]]
-	[[Category: Hearnshaw~~, S J~~]]	+	[[Category: Hearnshaw SJ]]
-	[[Category: Lawson~~, D M~~]]	+	[[Category: Lawson DM]]
-	[[Category: Maxwell, A]]	+	[[Category: Maxwell A]]
-	[[Category: Serebryakova, M]]	+	[[Category: Serebryakova M]]
-	[[Category: Severinov, K]]	+	[[Category: Severinov K]]
-	[[Category: Stevenson~~, C E.M~~]]	+	[[Category: Stevenson CEM]]
-	[[Category: Volkov, D]]	+	[[Category: Volkov D]]
-	~~[[Category: Ccda]]~~	+
-	~~[[Category: Dna gyrase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metalloprotease]]~~	+
-	~~[[Category: Microcin~~]]	+

OCA at 12:06, 26 August 2020

OCA — Wed, 26 Aug 2020 12:06:02 GMT

←Older revision		Revision as of 12:06, 26 August 2020
Line 1:		Line 1:

	==E. coli Microcin-processing metalloprotease TldD/E with DRVY angiotensin fragment bound==		==E. coli Microcin-processing metalloprotease TldD/E with DRVY angiotensin fragment bound==
-	<StructureSection load='5nj9' size='340' side='right' caption='[[5nj9]], [[Resolution\|resolution]] 1.25Å' scene=''>	+	<StructureSection load='5nj9' size='340' side='right'caption='[[5nj9]], [[Resolution\|resolution]] 1.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nj9]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NJ9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nj9]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NJ9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj9 OCA], [http://pdbe.org/5nj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5nj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5nj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj9 OCA], [http://pdbe.org/5nj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5nj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ghilarov, D]]		[[Category: Ghilarov, D]]
	[[Category: Hearnshaw, S J]]		[[Category: Hearnshaw, S J]]

OCA at 09:25, 4 October 2017

OCA — Wed, 04 Oct 2017 09:25:47 GMT

←Older revision		Revision as of 09:25, 4 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5nj9 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==E. coli Microcin-processing metalloprotease TldD/E with DRVY angiotensin fragment bound==
		+	<StructureSection load='5nj9' size='340' side='right' caption='[[5nj9]], [[Resolution\|resolution]] 1.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5nj9]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NJ9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nj9 OCA], [http://pdbe.org/5nj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nj9 RCSB], [http://www.ebi.ac.uk/pdbsum/5nj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nj9 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TLDD_ECOLI TLDD_ECOLI]] Metalloprotease involved in CcdA degradation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA).<ref>PMID:12029038</ref> [[http://www.uniprot.org/uniprot/PMBA_ECOLI PMBA_ECOLI]] Metalloprotease involved in CcdA degradation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA).<ref>PMID:12029038</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	TldD and TldE proteins are involved in the biosynthesis of microcin B17 (MccB17), an Escherichia coli thiazole/oxazole-modified peptide toxin targeting DNA gyrase. Using a combination of biochemical and crystallographic methods we show that E. coli TldD and TldE interact to form a heterodimeric metalloprotease. TldD/E cleaves the N-terminal leader sequence from the modified MccB17 precursor peptide, to yield mature antibiotic, while it has no effect on the unmodified peptide. Both proteins are essential for the activity; however, only the TldD subunit forms a novel metal-containing active site within the hollow core of the heterodimer. Peptide substrates are bound in a sequence-independent manner through beta sheet interactions with TldD and are likely cleaved via a thermolysin-type mechanism. We suggest that TldD/E acts as a "molecular pencil sharpener": unfolded polypeptides are fed through a narrow channel into the active site and processively truncated through the cleavage of short peptides from the N-terminal end.

-	~~Authors~~:	+	The Origins of Specificity in the Microcin-Processing Protease TldD/E.,Ghilarov D, Serebryakova M, Stevenson CEM, Hearnshaw SJ, Volkov D, Maxwell A, Lawson DM, Severinov K Structure. 2017 Sep 11. pii: S0969-2126(17)30259-9. doi:, 10.1016/j.str.2017.08.006. PMID:28943336<ref>PMID:28943336</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5nj9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ghilarov, D]]
		+	[[Category: Hearnshaw, S J]]
		+	[[Category: Lawson, D M]]
		+	[[Category: Maxwell, A]]
		+	[[Category: Serebryakova, M]]
		+	[[Category: Severinov, K]]
		+	[[Category: Stevenson, C E.M]]
		+	[[Category: Volkov, D]]
		+	[[Category: Ccda]]
		+	[[Category: Dna gyrase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Metalloprotease]]
		+	[[Category: Microcin]]

OCA: Protected "5nj9" [edit=sysop:move=sysop]

OCA — Wed, 05 Apr 2017 10:12:44 GMT

Protected "5nj9" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:12, 5 April 2017

OCA: New page: '''Unreleased structure''' The entry 5nj9 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 05 Apr 2017 10:12:43 GMT

New page: '''Unreleased structure''' The entry 5nj9 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5nj9 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]