5njl - Revision history

OCA at 13:01, 15 November 2023

OCA — Wed, 15 Nov 2023 13:01:44 GMT

←Older revision		Revision as of 13:01, 15 November 2023
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	==Cwp2 from Clostridium difficile==		==Cwp2 from Clostridium difficile==
-	<StructureSection load='5njl' size='340' side='right' caption='[[5njl]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5njl' size='340' side='right'caption='[[5njl]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5njl]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Clostridioides_difficile_r20291~~ Clostridioides difficile ~~r20291~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NJL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5njl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile_R20291 Clostridioides difficile R20291]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NJL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CDR20291_2680 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=645463 Clostridioides difficile R20291])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5njl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5njl OCA], [~~http~~://pdbe.org/5njl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5njl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5njl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5njl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5njl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5njl OCA], [https://pdbe.org/5njl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5njl RCSB], [https://www.ebi.ac.uk/pdbsum/5njl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5njl ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Clostridioides difficile ~~r20291~~]]	+	[[Category: Clostridioides difficile R20291]]
-	[[Category: ~~Acharya, K R~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Bradshaw, W J~~]]	+	[[Category: Acharya KR]]
-	[[Category: ~~Kirby, J M~~]]	+	[[Category: Bradshaw WJ]]
-	[[Category: ~~Roberts, A K~~]]	+	[[Category: Kirby JM]]
-	[[Category: ~~Shone, C C~~]]	+	[[Category: Roberts AK]]
-	[[Category: ~~Adhesin]]~~	+	[[Category: Shone CC]]
-	~~[[Category: Antibiotic associated diarrhoea]]~~	+
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Cell wall]]~~	+
-	~~[[Category: S-layer~~]]	+

OCA at 06:59, 31 January 2018

OCA — Wed, 31 Jan 2018 06:59:19 GMT

←Older revision		Revision as of 06:59, 31 January 2018
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	<StructureSection load='5njl' size='340' side='right' caption='[[5njl]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5njl' size='340' side='right' caption='[[5njl]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5njl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NJL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5njl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridioides_difficile_r20291 Clostridioides difficile r20291]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NJL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDR20291_2680 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=645463 Clostridioides difficile R20291])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5njl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5njl OCA], [http://pdbe.org/5njl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5njl RCSB], [http://www.ebi.ac.uk/pdbsum/5njl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5njl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5njl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5njl OCA], [http://pdbe.org/5njl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5njl RCSB], [http://www.ebi.ac.uk/pdbsum/5njl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5njl ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Clostridioides difficile r20291]]
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]
-	[[Category: ~~Brashaw~~, W J]]	+	[[Category: Bradshaw, W J]]
	[[Category: Kirby, J M]]		[[Category: Kirby, J M]]
	[[Category: Roberts, A K]]		[[Category: Roberts, A K]]

OCA at 10:31, 13 September 2017

OCA — Wed, 13 Sep 2017 10:31:25 GMT

←Older revision		Revision as of 10:31, 13 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5njl is ~~ON HOLD until Paper~~ Publication	+	==Cwp2 from Clostridium difficile==
		+	<StructureSection load='5njl' size='340' side='right' caption='[[5njl]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5njl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NJL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NJL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5njl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5njl OCA], [http://pdbe.org/5njl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5njl RCSB], [http://www.ebi.ac.uk/pdbsum/5njl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5njl ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Colonization of the gut by Clostridium difficile requires the adhesion of the bacterium to host cells. A range of cell surface located factors have been linked to adhesion including the S-layer protein LMW SLP and the related protein Cwp66. As well as these proteins, the S-layer of C. difficile may contain many others. One such protein is Cwp2. Here, we demonstrate the production of a C. difficile strain 630 cwp2 knockout mutant and assess the effect on the bacterium. The mutant results in increased TcdA (toxin A) release and impaired cellular adherence in vitro. We also present the extended three domain structure of the 'functional' region of Cwp2, consisting of residues 29-318 at 1.9 A, which is compared to that of LMW SLP and Cwp8. The adhesive properties of Cwp2 and LMW SLP, which are likely to be shared by Cwp8, are predicted to be mediated by the variable loop regions in domain 2. DATABASES: Structural data are available in the PDB under the accession number 5NJL.

-	~~Authors: Brashaw~~, ~~W.J.~~, Kirby~~, J.M.~~, Roberts~~, A.K.~~, Shone, C.C.~~, Acharya, K~~.R.	+	Cwp2 from Clostridium difficile exhibits an extended three domain fold and cell adhesion in vitro.,Bradshaw WJ, Kirby JM, Roberts AK, Shone CC, Acharya KR FEBS J. 2017 Sep;284(17):2886-2898. doi: 10.1111/febs.14157. Epub 2017 Jul 23. PMID:28677344<ref>PMID:28677344</ref>

-	~~Description: Cwp2 from Clostridium difficile~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Shone, C.C]]~~	+	<div class="pdbe-citations 5njl" style="background-color:#fffaf0;"></div>
-	[[Category: Acharya, K.R]]	+	== References ==
-	[[Category: Brashaw, W.J]]	+	<references/>
-	[[Category: Kirby, J.M]]	+	__TOC__
-	[[Category: Roberts, A.K]]	+	</StructureSection>
		+	[[Category: Acharya, K R]]
		+	[[Category: Brashaw, W J]]
		+	[[Category: Kirby, J M]]
		+	[[Category: Roberts, A K]]
		+	[[Category: Shone, C C]]
		+	[[Category: Adhesin]]
		+	[[Category: Antibiotic associated diarrhoea]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Cell wall]]
		+	[[Category: S-layer]]

OCA: Protected "5njl" [edit=sysop:move=sysop]

OCA — Wed, 05 Apr 2017 10:13:22 GMT

Protected "5njl" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:13, 5 April 2017

OCA: New page: '''Unreleased structure''' The entry 5njl is ON HOLD until Paper Publication Authors: Brashaw, W.J., Kirby, J.M., Roberts, A.K., Shone, C.C., Acharya, K.R. Description: Cwp2 from Clost...

OCA — Wed, 05 Apr 2017 10:13:21 GMT

New page: '''Unreleased structure''' The entry 5njl is ON HOLD until Paper Publication Authors: Brashaw, W.J., Kirby, J.M., Roberts, A.K., Shone, C.C., Acharya, K.R. Description: Cwp2 from Clost...

New page

'''Unreleased structure'''

The entry 5njl is ON HOLD until Paper Publication

Authors: Brashaw, W.J., Kirby, J.M., Roberts, A.K., Shone, C.C., Acharya, K.R.

Description: Cwp2 from Clostridium difficile
[[Category: Unreleased Structures]]
[[Category: Shone, C.C]]
[[Category: Acharya, K.R]]
[[Category: Brashaw, W.J]]
[[Category: Kirby, J.M]]
[[Category: Roberts, A.K]]