5nko - Revision history

OCA at 10:21, 14 June 2023

OCA — Wed, 14 Jun 2023 10:21:33 GMT

←Older revision		Revision as of 10:21, 14 June 2023
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	==Solution structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)==		==Solution structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)==
-	<StructureSection load='5nko' size='340' side='right'caption='[[5nko~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='5nko' size='340' side='right'caption='[[5nko]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nko]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Staa8 Staa8~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NKO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nko]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NKO FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hpf, SAOUHSC_00767 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nko OCA], [https://pdbe.org/5nko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nko RCSB], [https://www.ebi.ac.uk/pdbsum/5nko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nko ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nko OCA], [~~http~~://pdbe.org/5nko PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nko RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nko PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nko ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HPF_STAA8 HPF_STAA8]] Required for dimerization of active 70S ribosomes into 100S ribosomes; when added to monomeric 70S ribosomes stimulates formation of 100S dimeric ribosomes. Unlike E.coli, 100S ribosomes are present during exponential growth, peak during early stationary phase and then decrease (shown for strain NBRC 3060).[HAMAP-Rule:MF_00839]<ref>PMID:20015224</ref>	+	[https://www.uniprot.org/uniprot/HPF_STAA8 HPF_STAA8] Required for dimerization of active 70S ribosomes into 100S ribosomes; when added to monomeric 70S ribosomes stimulates formation of 100S dimeric ribosomes. Unlike E.coli, 100S ribosomes are present during exponential growth, peak during early stationary phase and then decrease (shown for strain NBRC 3060).[HAMAP-Rule:MF_00839]<ref>PMID:20015224</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staa8~~]]	+	[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
-	[[Category: Ayupov~~, R K~~]]	+	[[Category: Ayupov RK]]
-	[[Category: Khusainov~~, I S~~]]	+	[[Category: Khusainov IS]]
-	[[Category: Kieffer, B]]	+	[[Category: Kieffer B]]
-	[[Category: Usachev~~, K S~~]]	+	[[Category: Usachev KS]]
-	[[Category: Validov~~, S Z~~]]	+	[[Category: Validov SZ]]
-	[[Category: Yusupov~~, M M]]~~	+	[[Category: Yusupov MM]]
-	~~[[Category: Hibernating promoting factor]]~~	+
-	~~[[Category: Hibernation]]~~	+
-	~~[[Category: Hpf]]~~	+
-	~~[[Category: Pathogen]]~~	+
-	~~[[Category: Ribosomal protein]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Staphylococcus aureus~~]]	+

OCA at 13:13, 10 May 2019

OCA — Fri, 10 May 2019 13:13:55 GMT

←Older revision		Revision as of 13:13, 10 May 2019
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	==Solution structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)==		==Solution structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)==
-	<StructureSection load='5nko' size='340' side='right' caption='[[5nko]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='5nko' size='340' side='right'caption='[[5nko]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5nko]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staa8 Staa8]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NKO FirstGlance]. <br>		<table><tr><td colspan='2'>[[5nko]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staa8 Staa8]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NKO FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Staa8]]		[[Category: Staa8]]
	[[Category: Ayupov, R K]]		[[Category: Ayupov, R K]]

OCA at 06:04, 16 November 2017

OCA — Thu, 16 Nov 2017 06:04:50 GMT

←Older revision		Revision as of 06:04, 16 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5nko is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Solution structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)==
		+	<StructureSection load='5nko' size='340' side='right' caption='[[5nko]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5nko]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staa8 Staa8]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NKO FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hpf, SAOUHSC_00767 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nko OCA], [http://pdbe.org/5nko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nko RCSB], [http://www.ebi.ac.uk/pdbsum/5nko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nko ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HPF_STAA8 HPF_STAA8]] Required for dimerization of active 70S ribosomes into 100S ribosomes; when added to monomeric 70S ribosomes stimulates formation of 100S dimeric ribosomes. Unlike E.coli, 100S ribosomes are present during exponential growth, peak during early stationary phase and then decrease (shown for strain NBRC 3060).[HAMAP-Rule:MF_00839]<ref>PMID:20015224</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In bacteria, ribosomal hibernation shuts down translation as a response to stress, through reversible binding of stress-induced proteins to ribosomes. This process typically involves the formation of 100S ribosome dimers. Here, we present the structures of hibernating ribosomes from human pathogen Staphylococcus aureus containing a long variant of the hibernation-promoting factor (SaHPF) that we solved using cryo-electron microscopy. Our reconstructions reveal that the N-terminal domain (NTD) of SaHPF binds to the 30S subunit as observed for shorter variants of HPF in other species. The C-terminal domain (CTD) of SaHPF protrudes out of each ribosome in order to mediate dimerization. Using NMR, we characterized the interactions at the CTD-dimer interface. Secondary interactions are provided by helix 26 of the 16S ribosomal RNA We also show that ribosomes in the 100S particle adopt both rotated and unrotated conformations. Overall, our work illustrates a specific mode of ribosome dimerization by long HPF, a finding that may help improve the selectivity of antimicrobials.

-	~~Authors: Usachev, K.S~~., Khusainov, I~~.S.~~, Ayupov, R.K., Validov, S~~.Z.~~, Kieffer, B., Yusupov, M.M.	+	Structures and dynamics of hibernating ribosomes from Staphylococcus aureus mediated by intermolecular interactions of HPF.,Khusainov I, Vicens Q, Ayupov R, Usachev K, Myasnikov A, Simonetti A, Validov S, Kieffer B, Yusupova G, Yusupov M, Hashem Y EMBO J. 2017 Jul 14;36(14):2073-2087. doi: 10.15252/embj.201696105. Epub 2017 Jun, 23. PMID:28645916<ref>PMID:28645916</ref>

-	~~Description: Solution structure~~ of the ~~C-terminal domain of~~ S. ~~aureus Hibernating Promoting Factor (CTD~~-~~SaHPF)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5nko" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Staa8]]
		+	[[Category: Ayupov, R K]]
		+	[[Category: Khusainov, I S]]
	[[Category: Kieffer, B]]		[[Category: Kieffer, B]]
-	[[Category: ~~Yusupov~~, ~~M.M~~]]	+	[[Category: Usachev, K S]]
-	[[Category: Validov, S.Z]]	+	[[Category: Validov, S Z]]
-	[[Category: ~~Khusainov~~, ~~I.S~~]]	+	[[Category: Yusupov, M M]]
-	[[Category: ~~Ayupov, R.K~~]]	+	[[Category: Hibernating promoting factor]]
-	[[Category: ~~Usachev, K.S~~]]	+	[[Category: Hibernation]]
		+	[[Category: Hpf]]
		+	[[Category: Pathogen]]
		+	[[Category: Ribosomal protein]]
		+	[[Category: Ribosome]]
		+	[[Category: Staphylococcus aureus]]

OCA: Protected "5nko" [edit=sysop:move=sysop]

OCA — Wed, 12 Apr 2017 08:06:04 GMT

Protected "5nko" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:06, 12 April 2017

OCA: New page: '''Unreleased structure''' The entry 5nko is ON HOLD until Paper Publication Authors: Usachev, K.S., Khusainov, I.S., Ayupov, R.K., Validov, S.Z., Kieffer, B., Yusupov, M.M. Descriptio...

OCA — Wed, 12 Apr 2017 08:06:02 GMT

New page: '''Unreleased structure''' The entry 5nko is ON HOLD until Paper Publication Authors: Usachev, K.S., Khusainov, I.S., Ayupov, R.K., Validov, S.Z., Kieffer, B., Yusupov, M.M. Descriptio...

New page

'''Unreleased structure'''

The entry 5nko is ON HOLD until Paper Publication

Authors: Usachev, K.S., Khusainov, I.S., Ayupov, R.K., Validov, S.Z., Kieffer, B., Yusupov, M.M.

Description: Solution structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)
[[Category: Unreleased Structures]]
[[Category: Kieffer, B]]
[[Category: Yusupov, M.M]]
[[Category: Validov, S.Z]]
[[Category: Khusainov, I.S]]
[[Category: Ayupov, R.K]]
[[Category: Usachev, K.S]]